FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8071457
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG105513713.07244776252912No Hit
CAACGGAATCCCAAAAGCAGCTG4726175.855411234923261No Hit
CCCCCACTGCTAAATTTGACTG3942054.883938550375725No Hit
TAGCAGCACGTAAATATTGGCG3010753.7301196054194428No Hit
AACATTCAACGCTGTCGGTGAGT2894533.586130732035121No Hit
TTCAAGTAATCCAGGATAGGCT2595123.21518159608606No Hit
AAGCTGCCAGTTGAAGAACTGT2214572.7437053805775093No Hit
AACATTCAACGCTGTCGGTGAG1763512.184871950627997No Hit
TAGCTTATCAGACTGATGTTGA979801.213907228893123No Hit
TAGCTTATCAGACTGATGTTGAC928291.1500897545511302No Hit
TATTGCACTTGTCCCGGCCTGTAA919171.138790679303625No Hit
TGAGGTAGTAGGTTGTATAGTT912411.13041548756315No Hit
TAGCTTATCAGACTGATGTTGACT862931.0691130486106784No Hit
TATTGCACTTGTCCCGGCCTGTAT861581.0674404881299622No Hit
TATTGCACTTGTCCCGGCCTGT843491.0450281776883654No Hit
TTCAAGTAATTCAGGATAGGTT691310.8564872488325218No Hit
CATAAAGTAGAAAGCACTACT690420.8553845978489386No Hit
TGTAAACATCCTTGACTGGAAGCT611770.7579424631761031No Hit
TTCTCACTACTGCACTTGACTAG606310.7511778852318732No Hit
TGAGGTAGTAGATTGTATAGTT586490.7266222195075808No Hit
TATTGCACTTGTCCCGGCCTGTA512670.6351641345546412No Hit
AGCAGCATTGTACAGGGCTATGA455690.5645696929315239No Hit
TGTAAACATCCCCGACTGGAAGCT398130.49325666976854365No Hit
CTTCTCACTACTGCACTTGACTAG339810.4210020570016046No Hit
CCCCCCACTGCTAAATTTGACTGG311770.3862623563502847No Hit
CATTGCACTTGTCTCGGTCTGA305690.3787296395186148No Hit
ACTGGACTTGGAGTCAGAAGGC273620.33899703609893483No Hit
CGCCTGTCTGAGCGTCGCT272710.33786960644156316No Hit
CCCATAAAGTAGAAAGCACT269190.3335085598548069No Hit
TTAATGCTAATCGTGATAGGGGTT262840.32564133092699377No Hit
TGAGGTAGTAGTTTGTACAGTT258620.3204130307576439No Hit
CAACGGAATCCCAAAAGCAGCTGT243480.30165557469983423No Hit
TGATGTAGTAGGTTGTATAGTT237020.2936520630661849No Hit
TGAGAACTGAATTCCATGGGTT209560.25963094395472835No Hit
GACCCGCGGGCGCTCTCCAGTCCT204980.2539566276571875No Hit
CCCATAAAGTAGAAAGCACTAC202780.2512309735404649No Hit
CAACACCAGTCGATGGGCTGT196470.24341330196022848No Hit
CTCCCACTGCTTCACTTGACTAG183430.2272576066501996No Hit
AACATTCAACGCTGTCGGTGAGTT176370.21851073480289868No Hit
TAGCAGCACGTAAATATTGGC167330.2073107742505473No Hit
TGTAAACATCCTACACTCTCAGCT164070.2032718504230401No Hit
TGTCAGTTTGTCAAATACCCCAA162900.2018222980064194No Hit
TGAGGTAGTAGTTTGTGCTGTT159730.1978948782109599No Hit
TGTCAGTTTGTCAAATACCCCA156070.19336038090768495No Hit
TGATGTAGTAGATTGTATAGTT149320.18499757850410403No Hit
ACCGGGTGCTGTAGGCTT145780.18061175324355938No Hit
TGTAAACATCCCCGACTGGAAGC141720.17558168246451664No Hit
TATTGCACTTGTCCCGGCCTGTT140110.1735869992245514No Hit
TTCAAGTAATCCAGGATAGGCTT138210.17123302521465456No Hit
CAAAGAATTCTCCTTTTGGGCT135670.16808613364352928No Hit
CAACGGAATCCCAAAAGCAGCT131400.1627958867897085No Hit
ATCCCGGACGAGCCCCCA128770.15953749118653546No Hit
CCCCCACTGCTAAATTTGACTGG128540.159252536437969No Hit
ACCCCCCACTGCTAAATTTGACTG128180.15880652030977802No Hit
AGCTCGGTCTGAGGCCCCTCAGT121480.15050566459066808No Hit
CAAAGAATTCTCCTTTTGGGCTT120130.1488331041099519No Hit
TTATAAAGCAATGAGACTGATT115880.14356763592991947No Hit
TTCACAGTGGCTAAGTTCCG115760.14341896388718914No Hit
TAGCTTATCAGACTGATGTTGACA113510.1406313630859955No Hit
ACCGGGTGCTGTAGGCTTT112320.13915703199558643No Hit
AACATTCAACGCTGTCGGTGA112230.13904552796353867No Hit
TGAGAACTGAATTCCATAGGCTGT111740.13843845045572317No Hit
TTCAAGTAATCCAGGATAGGC111690.1383765037712522No Hit
CACTAGATTGTGAGCTCCTGGA110430.13681544732258377No Hit
TATTGCACTTGTCCCGGCCTGTAG109110.13518005485255016No Hit
AACCGGGCGGAAACACCA107890.1336685557514585No Hit
ACCGGGTGCTGTAGGCTTA105930.1312402457201965No Hit
AACGGAATCCCAAAAGCAGCTG105800.13107918434057197No Hit
CCCCCACTGCTAAATTTGACTGGCTT104180.12907211176371253No Hit
CTACGCCTGTCTGAGCGTCGCT102580.12708981786064152No Hit
GGAATACCGGGTGCTGTAGGCTTT102480.12696592449169958No Hit
CCCCCCACTGCTAAATTTGACTGT101090.12524380666340662No Hit
GGAATACCGGGTGCTGTAGGCTT96410.11944559699692385No Hit
CGCGACCTCAGATCAGACGTGGCG96110.11907391689009804No Hit
AGCAGCATTGTACAGGGCTATG95090.11781020452689024No Hit
TTCACAGTGGCTAAGTTCTG93730.11612525470927988No Hit
CAAAGTGCTGTTCGTGCAGGT93490.11582791062381922No Hit
TGAGGTAGTAGGTTGTGTGGTT92510.1146137556081882No Hit
GCCTGTCTGAGCGTCGCT90160.11170226143805263No Hit
AATGACACGATCACTCCCGTTG88670.10985625024081773No Hit
TTCACAGTGGCTAAGTTCTGC87170.10799784970668865No Hit
TGAGGTAGTAGGTTGTATAGT87080.1078863456746409No Hit
AGCAGCATTGTACAGGGCTATGT87030.10782439899016992No Hit
CACCCGGCTGTGTGCACATGTGC84790.10504918752587049No Hit
CACTAGATTGTGAGCTCCTGGAA83510.10346335240341367No Hit
CCCCCCACTGCTAAATTTGACTGGCT83480.10342618439273107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)