FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6786642
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG139679920.58159248712397No Hit
CCCCCACTGCTAAATTTGACTG5946678.762315737296884No Hit
CAACGGAATCCCAAAAGCAGCTG5012537.385876549846006No Hit
AACATTCAACGCTGTCGGTGAGT1903562.8048628467510146No Hit
GGAATACCGGGTGCTGTAGGCTTT1826542.691375204408896No Hit
AACATTCAACGCTGTCGGTGAG1556792.293903229314291No Hit
TTCAAGTAATCCAGGATAGGCT1307231.9261808711878423No Hit
GGAATACCGGGTGCTGTAGGCTT1226021.8065193360722431No Hit
TAGCAGCACGTAAATATTGGCG1128801.663267341934347No Hit
AAGCTGCCAGTTGAAGAACTGT1126401.6597309833051455No Hit
TATTGCACTTGTCCCGGCCTGTAA1095881.6147602894038025No Hit
TAGCTTATCAGACTGATGTTGACT992651.4626526638652813No Hit
TAGCTTATCAGACTGATGTTGAC940861.3863409916126415No Hit
TATTGCACTTGTCCCGGCCTGTAT925911.3643124243182416No Hit
TGAGGTAGTAGGTTGTATAGTT726941.0711335591298319No Hit
TAGCTTATCAGACTGATGTTGA721351.062896790489317No Hit
TATTGCACTTGTCCCGGCCTGT476430.702011392379324No Hit
TATTGCACTTGTCCCGGCCTGTA470310.6929936778748607No Hit
TGAGGTAGTAGATTGTATAGTT432690.6375612563621301No Hit
AGCAGCATTGTACAGGGCTATGA378490.5576984906526674No Hit
TGTAAACATCCTTGACTGGAAGCT338720.49909808120127747No Hit
TTCAAGTAATTCAGGATAGGTT283810.418189142730676No Hit
GGAATACCGGGTGCTGTAGGCTTA281270.4144464965147712No Hit
TGTAAACATCCCCGACTGGAAGCT277430.4087883227080491No Hit
CATAAAGTAGAAAGCACTACT253080.37290901744927757No Hit
ACTGGACTTGGAGTCAGAAGGC248460.36610152708806504No Hit
TTAATGCTAATCGTGATAGGGGTT235570.3471083342837297No Hit
CATTGCACTTGTCTCGGTCTGA232930.3432183397916083No Hit
CAACGGAATCCCAAAAGCAGCTGT218660.3221917407754822No Hit
CAACGGAATCCCAAAAGCAGCT209050.3080315714310553No Hit
GACCCGCGGGCGCTCTCCAGTCCT200640.29563958140122903No Hit
TGATGTAGTAGGTTGTATAGTT191900.2827613420598877No Hit
GGAATACCGGGTGCTGTAGGCT191820.2826434634389143No Hit
TTCTCACTACTGCACTTGACTAG181510.26745185616097034No Hit
CCCATAAAGTAGAAAGCACTAC146010.21514321810403436No Hit
ACCCCCCACTGCTAAATTTGACTG145020.2136844701694888No Hit
TAGCTTATCAGACTGATGTTGACA142160.20947030946969059No Hit
CCCCCACTGCTAAATTTGACTGGCTT141100.2079084177417934No Hit
CCCCCCACTGCTAAATTTGACTGG136170.200644147724309No Hit
CCCATAAAGTAGAAAGCACT133240.1963268432311591No Hit
CACTAGATTGTGAGCTCCTGGA132790.19566377598818385No Hit
TGAGGTAGTAGTTTGTACAGTT131070.19312938563725623No Hit
CCCGCCACTGCTAAATTTGACTG130830.19277574977433612No Hit
CTTCTCACTACTGCACTTGACTAG128650.1895635573528116No Hit
AACATTCAACGCTGTCGGTGAGTT127370.18767749941723755No Hit
CACTAGATTGTGAGCTCCTGGAA121810.17948493525958786No Hit
TATTGCACTTGTCCCGGCCTGTAG118730.17494660835211287No Hit
CAACACCAGTCGATGGGCTGT116530.17170494627534502No Hit
TGATGTAGTAGATTGTATAGTT112670.16601730281337956No Hit
CAAAGAATTCTCCTTTTGGGCT110370.162628292460395No Hit
CTCCCCACTGCTAAATTTGACTG108040.1591950776245454No Hit
CACCCGGCTGTGTGCACATGTGC103800.15294751071295642No Hit
TGAGAACTGAATTCCATGGGTT102860.15156243691651924No Hit
TATTGCACTTGTCCCGGCCTGTT101730.1498974013952703No Hit
TGAATACCGGGTGCTGTAGGCTTT101410.1494258869113768No Hit
TGAGAACTGAATTCCATAGGCTGT101130.14901331173796997No Hit
TAGCAGCACGTAAATATTGGC99590.14674414828423246No Hit
TATTGCACTTGTCCCGGCCTGTAAT98210.14471074207244172No Hit
TGAGGTAGTAGTTTGTGCTGTT96610.14235316965297418No Hit
GTAATACCGGGTGCTGTAGGCTTT94090.1386399930923128No Hit
AACGGAATCCCAAAAGCAGCTG93620.13794745619409424No Hit
TTATAAAGCAATGAGACTGATT93000.13703389688155054No Hit
CGCCCCACTGCTAAATTTGACTG92310.1360171937756552No Hit
TGTAAACATCCTACACTCTCAGCT91500.13482367273829973No Hit
CCCCCCACTGCTAAATTTGACTGT87430.1288265978962792No Hit
CTCCCACTGCTTCACTTGACTAG83530.12308001512382707No Hit
TATTGCACTTGTCCCGGCCTGTAAA82920.12218119063890508No Hit
CAAAGAATTCTCCTTTTGGGCTT81550.120162519254736No Hit
TTCAAGTAATCCAGGATAGGCTT80570.11871850614781213No Hit
AGCAGCATTGTACAGGGCTATGT80190.11815858269818859No Hit
ACTGGACTTGGAGTCAGAAGGCA77140.1136644602735786No Hit
TCGTACGACTCTTAGCGG74640.10998075336816059No Hit
CCCCCCACTGCTAAATTTGACTGTA73430.10819783922593824No Hit
ACTGGACTTGGAGTCAGAAGGCT70590.10401314818138338No Hit
TCAGTGCACTACAGAACTTTGT70180.10340902024889483No Hit
ACTTGACTTGGAGTCAGAAGGC69170.10192080265910594No Hit
TGAATACCGGGTGCTGTAGGCTT68470.10088936472558889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)