FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11043573
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG164425714.888813611319451No Hit
CCCCCACTGCTAAATTTGACTG5744225.201414433535233No Hit
CAACGGAATCCCAAAAGCAGCTG5186154.696079792291861No Hit
AACATTCAACGCTGTCGGTGAGT3698153.3486897763975483No Hit
TTCAAGTAATCCAGGATAGGCT3484973.1556544245236573No Hit
TAGCAGCACGTAAATATTGGCG3375683.0566918876707745No Hit
AAGCTGCCAGTTGAAGAACTGT3122582.82750881440273No Hit
TAGCTTATCAGACTGATGTTGAC1876891.6995314831531425No Hit
AACATTCAACGCTGTCGGTGAG1865631.6893355076296412No Hit
TAGCTTATCAGACTGATGTTGACT1508681.366115839502306No Hit
TAGCTTATCAGACTGATGTTGA1336621.2103148138740967No Hit
CATAAAGTAGAAAGCACTACT963850.8727700717874549No Hit
TATTGCACTTGTCCCGGCCTGTAA951010.8611433998761089No Hit
TATTGCACTTGTCCCGGCCTGTAT869990.7877794623171324No Hit
TGTAAACATCCTTGACTGGAAGCT795770.7205729522501458No Hit
TATTGCACTTGTCCCGGCCTGT792460.7175757338680154No Hit
TGAGGTAGTAGGTTGTATAGTT691140.6258300642373623No Hit
TATTGCACTTGTCCCGGCCTGTA622670.5638302024172792No Hit
AGCAGCATTGTACAGGGCTATGA511580.4632377582871051No Hit
TTCAAGTAATTCAGGATAGGTT494110.4474186026569481No Hit
TTCTCACTACTGCACTTGACTAG490480.44413162298107683No Hit
CCCATAAAGTAGAAAGCACTAC460630.417102327299326No Hit
TGTAAACATCCCCGACTGGAAGCT443610.40169064848849195No Hit
CGCCCCACTGCTAAATTTGACTG410960.37212594148650985No Hit
TTAATGCTAATCGTGATAGGGGTT388090.3514170640244783No Hit
ACTGGACTTGGAGTCAGAAGGC366000.3314144797159398No Hit
AACATTCAACGCTGTCGGTGAGTT361920.32772002322074567No Hit
TGAGGTAGTAGATTGTATAGTT361220.3270861703906879No Hit
CTTCTCACTACTGCACTTGACTAG338760.30674854958626163No Hit
CAACGGAATCCCAAAAGCAGCTGT331260.2999572692642137No Hit
CATTGCACTTGTCTCGGTCTGA296880.2688260402679459No Hit
CCCCCCACTGCTAAATTTGACTGG291380.2638457680317774No Hit
CGCCTGTCTGAGCGTCGCT277280.2510781610263273No Hit
CCCGCCACTGCTAAATTTGACTG275760.2497017948810589No Hit
TGTAAACATCCTACACTCTCAGCT272220.24649631056905225No Hit
CCCATAAAGTAGAAAGCACT258900.23443499671709508No Hit
TTCACAGTGGCTAAGTTCCG258410.23399129973605462No Hit
CAAAGAATTCTCCTTTTGGGCT248000.22456500264905208No Hit
TTTAAACATCCTTGACTGGAAGCT244070.22100637176029897No Hit
GACCCGCGGGCGCTCTCCAGTCCT234930.21273006480782986No Hit
CCCTCCACTGCTAAATTTGACTG231610.20972379138526998No Hit
TAGCTTATCAGACTGATGTTGACA220040.19924710960845735No Hit
TGATGTAGTAGGTTGTATAGTT216340.19589674464958037No Hit
TCGTACGACTCTTAGCGG205410.18599958546024914No Hit
CCCCCACTGCTAAATTTGACTGGCTT193560.17526936255141337No Hit
CTACGCCTGTCTGAGCGTCGCT189480.1715749060562193No Hit
TGTCAGTTTGTCAAATACCCCAA188780.1709410532261615No Hit
TTCAAGTAATCCAGGATAGGCTT187350.16964618244475768No Hit
CAACACCAGTCGATGGGCTGT180810.16372418600393188No Hit
CAAAGAATTCTCCTTTTGGGCTT179710.16272813155669819No Hit
ACTTGACTTGGAGTCAGAAGGC169120.15313884374196648No Hit
GCCCCCACTGCTAAATTTGACTG167010.15122823021136367No Hit
CTCCCACTGCTTCACTTGACTAG165430.1497975338235189No Hit
TATTGCACTTGTCCCGGCCTGTT160140.14500741743636775No Hit
TAGCAGCACGTAAATATTGGC159510.1444369498893157No Hit
TGCAAGTAATCCAGGATAGGCT158150.1432054643909177No Hit
TTATAAAGCAATGAGACTGATT157140.14229090530754857No Hit
CCCCCCACTGCTAAATTTGACTGGCT155550.1408511538792744No Hit
CAACGGAATCCCAAAAGCAGCT155370.14068816315154525No Hit
ACCCCCCACTGCTAAATTTGACTG152640.1382161371143198No Hit
TTCAAGTAATCCAGGATAGGC152550.13813464175045523No Hit
CTCCCCACTGCTAAATTTGACTG151170.1368850461711984No Hit
TGAGGTAGTAGTTTGTACAGTT149260.13515553344918352No Hit
TGTCAGTTTGTCAAATACCCCA138540.12544853010886967No Hit
TAGCAGCACGTAAATATTGGCGT138290.12522215409813472No Hit
TTTAAACATCCCCGACTGGAAGCT136640.12372807242728417No Hit
CACTAGATTGTGAGCTCCTGGA134890.12214344035213966No Hit
TACGCCTGTCTGAGCGTCGCT133900.12124699134962932No Hit
CGCCCACTGCTAAATTTGACTG131460.1190375614848564No Hit
ATCAGCATTGTACAGGGCTATGA129970.1176883604608762No Hit
TGTAAACATCCCCGACTGGAAGC128380.11624860903260205No Hit
CTATGCCTGTCTGAGCGTCGCT125720.11383996827838236No Hit
TGTAAACATCCTACACTCAGCT125460.11360453722721803No Hit
AACATTCAACGCTGTCGGTGA124150.112418326930967No Hit
TATTGCACTTGTCCCGGCCTGTAG121090.10964748455957143No Hit
TTAGGTAGTAGGTTGTATAGTT120430.1090498518912312No Hit
TTCACAGTGGCTAAGTTCTG120290.10892308132521966No Hit
TGAGGTAGTAGTTTGTGCTGTT119690.10837977889945581No Hit
CACTAGATTGTGAGCTCCTGGAA116050.10508374418315522No Hit
TTATGTAGTAGGTTGTATAGTT115230.10434123086794464No Hit
TGATGTAGTAGATTGTATAGTT114830.10397902925076874No Hit
AGCATTCAACGCTGTCGGTGAGT110620.1001668572299925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)