FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5948964
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG69567111.693985709108343No Hit
CAACGGAATCCCAAAAGCAGCTG3194815.370363646510552No Hit
CCCCCACTGCTAAATTTGACTG2747034.617661159153089No Hit
AACATTCAACGCTGTCGGTGAGT2001273.3640647346327865No Hit
TTCAAGTAATCCAGGATAGGCT1834003.082889726681822No Hit
TAGCAGCACGTAAATATTGGCG1718512.8887550840785052No Hit
AAGCTGCCAGTTGAAGAACTGT1638922.7549670833442597No Hit
AACATTCAACGCTGTCGGTGAG1005071.6894874468899121No Hit
TAGCTTATCAGACTGATGTTGAC772171.297990708970503No Hit
TAGCTTATCAGACTGATGTTGA621731.045106341204956No Hit
TAGCTTATCAGACTGATGTTGACT612701.0299272276651867No Hit
GCCTGTCTGAGCGTCGCT608911.0235563704873656No Hit
CATAAAGTAGAAAGCACTACT527120.8860702468530655No Hit
TGAGGTAGTAGGTTGTATAGTT512380.8612928234227001No Hit
TATTGCACTTGTCCCGGCCTGTAA471750.7929952173185113No Hit
TATTGCACTTGTCCCGGCCTGT448370.753694256680659No Hit
TGTAAACATCCTTGACTGGAAGCT415590.6985922254698465No Hit
CGCCTGTCTGAGCGTCGCT397790.6686710492784962No Hit
TATTGCACTTGTCCCGGCCTGTAT394280.6627708622879547No Hit
AGCAGCATTGTACAGGGCTATGA394090.6624514789465863No Hit
TATTGCACTTGTCCCGGCCTGTA284480.4782009102761422No Hit
CCCATAAAGTAGAAAGCACTAC249750.4198209974039177No Hit
TCCTGTCTGAGCGTCGCT245560.41277775424426844No Hit
ACTGGACTTGGAGTCAGAAGGC245000.41183641386970904No Hit
TGTAAACATCCCCGACTGGAAGCT238630.40112866710909667No Hit
TGAGGTAGTAGATTGTATAGTT237400.3990610802149752No Hit
TTCAAGTAATTCAGGATAGGTT233900.39317770287397946No Hit
TTCTCACTACTGCACTTGACTAG227400.38225143066927286No Hit
CAACGGAATCCCAAAAGCAGCTGT197310.33167119518625426No Hit
CTACGCCTGTCTGAGCGTCGCT175950.2957657837566339No Hit
AACATTCAACGCTGTCGGTGAGTT168190.2827214957091689No Hit
CCCCCACTGCTAAATTTGACTGGCTT164110.27586315869452227No Hit
CATTGCACTTGTCTCGGTCTGA159450.268029862006225No Hit
TGTAAACATCCTACACTCTCAGCT158470.2663825163507461No Hit
GACCCGCGGGCGCTCTCCAGTCCT151690.25498557395875987No Hit
CCCATAAAGTAGAAAGCACT150780.253455895850101No Hit
TACGCCTGTCTGAGCGTCGCT147540.24800956939729338No Hit
CTTCTCACTACTGCACTTGACTAG143970.24200852450947763No Hit
CCCCCCACTGCTAAATTTGACTGG143090.24052927534945578No Hit
TAGCAGCACATCATGGTTTACA142910.24022670165763316No Hit
ACGCCTGTCTGAGCGTCGCT125140.21035595441491997No Hit
CAACGGAATCCCAAAAGCAGCT120860.20316142440935936No Hit
CTCCCACTGCTTCACTTGACTAG120280.2021864647357086No Hit
CCCCCCACTGCTAAATTTGACTGGCT113610.19097442848872512No Hit
TTCACAGTGGCTAAGTTCCG112260.18870512580005527No Hit
GTCAGGATGGCCGAGCGGTCTAAGG110030.18495657395136364No Hit
TGATGTAGTAGGTTGTATAGTT109590.18421694937135275No Hit
CAAAGAATTCTCCTTTTGGGCT107840.18127526070085478No Hit
CAACACCAGTCGATGGGCTGT105410.17719051586124912No Hit
TAGCTTATCAGACTGATGTTGACA105100.17666941672533235No Hit
TAGCAGCACGTAAATATTGGC102250.17187866660480716No Hit
TGTAAACATCCTACACTCAGCT101090.16992874725750567No Hit
TGAGGTAGTAGTTTGTACAGTT98600.16574314452062577No Hit
CTATGCCTGTCTGAGCGTCGCT95000.15969167068417292No Hit
TTCAAGTAATCCAGGATAGGCTT93250.156749982013675No Hit
ACCGGGTGCTGTAGGCTT92130.1548673012645563No Hit
TTCACAGTGGCTAAGTTCCGC91120.15316952666044037No Hit
AGCTCGGTCTGAGGCCCCTCAGT86820.14594137735578835No Hit
ACCCCCCACTGCTAAATTTGACTG85270.14333588167620445No Hit
TTCAAGTAATCCAGGATAGGC84960.1428147825402877No Hit
TCGTTTCCCGGCCAATGCACCA83860.1409657210902604No Hit
TAGCAGCACGTAAATATTGGCGT83350.14010842896342962No Hit
CGCCCCACTGCTAAATTTGACTG83030.13957052017796712No Hit
TATTGCACTTGTCCCGGCCTGTT81650.13725078854066017No Hit
AGCAGCATTGTACAGGGCTATGT81440.13689778590020044No Hit
GCGTTCGCGCTTTCCCCTG80140.13471253145925913No Hit
TTCACAGTGGCTAAGTTCTGC78760.1323927998219522No Hit
ATCACATTGCCAGGGATTTCC78450.1318717006860354No Hit
TATGCCTGTCTGAGCGTCGCT78330.131669984891487No Hit
TTATAAAGCAATGAGACTGATT77490.13025797432964797No Hit
TGAGGTAGTAGTTTGTGCTGTT77300.12993859098827965No Hit
CAAAGAATTCTCCTTTTGGGCTT74580.12536636631184858No Hit
CTCCCCACTGCTAAATTTGACTG74110.12457631278320055No Hit
AGCAGCATTGTACAGGGCTATG72240.12143290831815423No Hit
TCGTACGACTCTTAGCGG71770.1206428547895062No Hit
TGAGGTAGTAGGTTGTGTGGTT70410.11835674245129067No Hit
TGTAAACATCCCCGACTGGAAGC69780.11729773452991143No Hit
AACGGAATCCCAAAAGCAGCTG66890.11243974581120343No Hit
ACCACAGGGTAGAACCACGGAC64550.10850628781750907No Hit
TGCAAGTAATCCAGGATAGGCT61930.10410215963653503No Hit
CAAAGTGCTGTTCGTGCAGGT60520.101731999050591No Hit
CCCGCCACTGCTAAATTTGACTG60510.10171518940104529No Hit
TGTCAGTTTGTCAAATACCCCA59820.10055532358239183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)