FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7502827
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6229598.302990326179719No Hit
CCCCCCACTGCTAAATTTGACTG5195796.925109695318844No Hit
AACATTCAACGCTGTCGGTGAGT3224394.29756677049864No Hit
TAGCAGCACGTAAATATTGGCG2837253.781574598481346No Hit
TTCAAGTAATCCAGGATAGGCT2361603.147613559528962No Hit
AAGCTGCCAGTTGAAGAACTGT2130412.8394763733723303No Hit
AACATTCAACGCTGTCGGTGAG1779722.372065889297461No Hit
CCCCCACTGCTAAATTTGACTG1754032.3378254623224017No Hit
TATTGCACTTGTCCCGGCCTGTAA1466861.9550763998690095No Hit
TATTGCACTTGTCCCGGCCTGTAT1167251.555746920460781No Hit
TAGCTTATCAGACTGATGTTGAC938661.2510750947609481No Hit
TAGCTTATCAGACTGATGTTGACT887231.1825275992635842No Hit
TATTGCACTTGTCCCGGCCTGT849741.13255976713844No Hit
TGAGGTAGTAGGTTGTATAGTT794211.0585476647668939No Hit
TAGCTTATCAGACTGATGTTGA673600.8977949244997918No Hit
TGTAAACATCCTTGACTGGAAGCT632740.8433354520902587No Hit
TTCAAGTAATTCAGGATAGGTT591930.7889426212279718No Hit
TGAGGTAGTAGATTGTATAGTT537820.7168231387982157No Hit
TATTGCACTTGTCCCGGCCTGTA537570.7164899310619851No Hit
GGAATACCGGGTGCTGTAGGCTTT498260.6640963466170818No Hit
TGTAAACATCCCCGACTGGAAGCT476720.6353871680634513No Hit
AGCAGCATTGTACAGGGCTATGA463310.6175139050920406No Hit
CATAAAGTAGAAAGCACTACT457650.6099700819437793No Hit
TTCTCACTACTGCACTTGACTAG412830.5502325989923532No Hit
GGAATACCGGGTGCTGTAGGCTT374610.4992918002774154No Hit
CACCCGGCTGTGTGCACATGTGC373620.4979722976419422No Hit
CAACGGAATCCCAAAAGCAGCTGT349820.46625092115278677No Hit
ACTGGACTTGGAGTCAGAAGGC331530.4418734431701544No Hit
CTTCTCACTACTGCACTTGACTAG318600.42463993905230657No Hit
TGATGTAGTAGGTTGTATAGTT301540.4019018431319288No Hit
CATTGCACTTGTCTCGGTCTGA278740.37151329758769597No Hit
TCGTACGACTCTTAGCGG250240.33352761565740485No Hit
CCCCCCACTGCTAAATTTGACTGG249920.3331011097550297No Hit
CGCCTGTCTGAGCGTCGCT248140.3307286706730676No Hit
AACATTCAACGCTGTCGGTGAGTT227110.30269923590134756No Hit
TGAGGTAGTAGTTTGTACAGTT223880.29839419194924793No Hit
CCCATAAAGTAGAAAGCACTAC218470.29118357653721727No Hit
TGAATACCGGGTGCTGTAGGCTTT203590.2713510520767705No Hit
TGATGTAGTAGATTGTATAGTT203360.2710445009594384No Hit
CCCCCACTGCTAAATTTGACTGGCTT199050.2652999995868224No Hit
CCCATAAAGTAGAAAGCACT182140.24276182830818305No Hit
CAACGGAATCCCAAAAGCAGCT174480.23255234327007676No Hit
TATTGCACTTGTCCCGGCCTGTAG163330.2176912782341909No Hit
TGTAAACATCCTACACTCTCAGCT163220.21754466683024948No Hit
CCCCCCACTGCTAAATTTGACTGGCT161170.21481236339315835No Hit
CTCCCACTGCTTCACTTGACTAG160830.2143592008718847No Hit
TAGCAGCACGTAAATATTGGC159670.21281311697577462No Hit
CAAAGAATTCTCCTTTTGGGCTT156220.208214850215792No Hit
TGAATACCGGGTGCTGTAGGCTT154650.20612230563226366No Hit
CAAAGAATTCTCCTTTTGGGCT152260.20293683967389892No Hit
TGTCAGTTTGTCAAATACCCCAA149770.19961809062104188No Hit
TGTAAACATCCCCGACTGGAAGC147300.19632599818708335No Hit
ACTTGACTTGGAGTCAGAAGGC140940.1878491933773763No Hit
TATTGCACTTGTCCCGGCCTGTT139940.1865163624324538No Hit
AGCTCGGTCTGAGGCCCCTCAGT133250.1775997234109223No Hit
CACTAGATTGTGAGCTCCTGGA132400.1764668171077382No Hit
CAACACCAGTCGATGGGCTGT130970.17456086885649902No Hit
TTCAAGTAATCCAGGATAGGCTT129350.1724016827257246No Hit
CTACGCCTGTCTGAGCGTCGCT125950.16787005751298809No Hit
TGAGGTAGTAGTTTGTGCTGTT125470.1672302986594253No Hit
CACTAGATTGTGAGCTCCTGGAA119480.15924664129933958No Hit
ACCCCCCACTGCTAAATTTGACTG117780.15698082869297134No Hit
AACGGAATCCCAAAAGCAGCTG116450.15520816353622444No Hit
TAGCTTATCAGACTGATGTTGACA116250.15494159734723992No Hit
TGTCAGTTTGTCAAATACCCCA113260.15095643282192164No Hit
TATTGCACTTGTCCCGGCCTGTAAT109580.1460516149446069No Hit
GCCTGTCTGAGCGTCGCT109320.14570507889892703No Hit
TTATAAAGCAATGAGACTGATT97140.12947119798977105No Hit
TATTGCACTTGTCCCGGCCTGTAAA95540.12733866847789507No Hit
TTCAAGTAATCCAGGATAGGC94560.126032494151871No Hit
TGCAAGTAATCCAGGATAGGCT93700.12488625953923768No Hit
AACATTCAACGCTGTCGGTGA92940.12387330802109658No Hit
CAACGGAATCCCAAAAGCAGCTGA90730.12092775163281787No Hit
AACCGGGCGGAAACACCA90660.12083445346667329No Hit
TATTGCACTTGTCCCGGCCTGTTT89820.1197148754729384No Hit
TGAGGTAGTAGGTTGTGTGGTT87420.11651608120512441No Hit
CCCCCACTGCTAAATTTGACTGG85850.1144235366215961No Hit
ACCGGGTGCTGTAGGCTT85810.11437022338379921No Hit
ACTGGACTTGGAGTCAGAAGGCT85170.11351721157904882No Hit
CAAAGTGCTGTTCGTGCAGGT85060.11337060017510732No Hit
ATCCCGGACGAGCCCCCA84910.11317067553336896No Hit
TGATGTAGTAGTTTGTACAGTT84910.11317067553336896No Hit
ACCACAGGGTAGAACCACGGAC84170.11218438063412631No Hit
CAAAGTGCTGTTCGTGCAGGTAG84020.11198445599238795No Hit
ACCGGGTGCTGTAGGCTTA83040.1106782816663639No Hit
GGAATACCGGGTGCTGTAGGCTTA81920.10918551100805071No Hit
GAATACCGGGTGCTGTAGGCTTT81440.1085457521544879No Hit
AGCAGCATTGTACAGGGCTATGT79850.10642655095206115No Hit
AACATTCAACGCTGTCGGTGAGA76480.10193491066767234No Hit
CTCCCACTGCTTCACTTGACTAGCCT75800.10102858562512504No Hit
TTCACAGTGGCTAAGTTCTGC75110.10010893227312852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)