FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9558894
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7466317.810851339077512No Hit
CCCCCCACTGCTAAATTTGACTG6882347.19993338141421No Hit
TTCAAGTAATCCAGGATAGGCT3417403.575099797110419No Hit
AACATTCAACGCTGTCGGTGAGT3047513.188140803737336No Hit
CCCCCACTGCTAAATTTGACTG2655872.7784281319575257No Hit
AAGCTGCCAGTTGAAGAACTGT2449092.562106034442897No Hit
TAGCAGCACGTAAATATTGGCG2181342.282000407160075No Hit
AACATTCAACGCTGTCGGTGAG1835861.9205778409092096No Hit
TGAGGTAGTAGGTTGTATAGTT1652821.7290912526072577No Hit
TATTGCACTTGTCCCGGCCTGTAA1446661.5134177656954872No Hit
TATTGCACTTGTCCCGGCCTGTAT1171651.2257171174824202No Hit
TAGCTTATCAGACTGATGTTGAC1089021.1392740624595272No Hit
TGAGGTAGTAGATTGTATAGTT1034631.0823741742507031No Hit
TATTGCACTTGTCCCGGCCTGT1008011.0545257641731354No Hit
TAGCTTATCAGACTGATGTTGACT1003711.0500273357984722No Hit
CATAAAGTAGAAAGCACTACT878620.9191649159411119No Hit
TAGCTTATCAGACTGATGTTGA793850.8304831081922238No Hit
TGTAAACATCCTTGACTGGAAGCT645910.6757162491811292No Hit
CCCATAAAGTAGAAAGCACTAC591170.61845020982553No Hit
TATTGCACTTGTCCCGGCCTGTA590340.6175819085346066No Hit
TCCACCACGTTCCCGTGG546080.5712794806595826No Hit
AGCAGCATTGTACAGGGCTATGA517350.541223702240029No Hit
ACTGGACTTGGAGTCAGAAGGC498930.5219536904583313No Hit
TTCAAGTAATTCAGGATAGGTT477960.5000160060358447No Hit
TGAGGTAGTAGTTTGTACAGTT464880.4863324146077988No Hit
CGCCTGTCTGAGCGTCGCT421030.4404589066475682No Hit
CACCCGGCTGTGTGCACATGTGC415640.4348201789872343No Hit
CATTGCACTTGTCTCGGTCTGA414950.43409833815502086No Hit
GGAATACCGGGTGCTGTAGGCTTT412050.43106451436745713No Hit
TGTAAACATCCCCGACTGGAAGCT381730.3993453635954118No Hit
CAACGGAATCCCAAAAGCAGCTGT373150.39036942976875777No Hit
CTACGCCTGTCTGAGCGTCGCT357350.37384032085720376No Hit
TTCTCACTACTGCACTTGACTAG337980.3535764702485455No Hit
GGAATACCGGGTGCTGTAGGCTT317660.33231878081292665No Hit
CCCATAAAGTAGAAAGCACT312870.32730774083277836No Hit
CCCCCACTGCTAAATTTGACTGGCTT308270.3224954686180221No Hit
TCGTACGACTCTTAGCGG278170.2910064699953781No Hit
GCCTGTCTGAGCGTCGCT274140.2867905010767982No Hit
ACCGGGTGCTGTAGGCTT270070.2825326863128726No Hit
CTCCCACTGCTTCACTTGACTAG259570.27154815190962467No Hit
TGAGGTAGTAGTTTGTGCTGTT259410.2713807685282419No Hit
TTCACAGTGGCTAAGTTCCGC250820.26239437324025144No Hit
CTTCTCACTACTGCACTTGACTAG248840.26032300389563895No Hit
TGTAAACATCCTACACTCTCAGCT240710.25181783582912415No Hit
ACCGGGTGCTGTAGGCTTA227290.2377785547156397No Hit
TAGCAGCACATCATGGTTTACA225480.2358850302137465No Hit
GTCAGGATGGCCGAGCGGTCTAAGG215480.2254235688773199No Hit
AACATTCAACGCTGTCGGTGAGTT213270.22311158592196964No Hit
CAACACCAGTCGATGGGCTGT211690.2214586750308142No Hit
TCAGGCTCAGTCCCCTCCCGAT211320.22107160096136647No Hit
GCCCCCACTGCTAAATTTGACTG209020.21866546485398833No Hit
ATCCCGGACGAGCCCCCA200040.20927107257387728No Hit
CCCCCCACTGCTAAATTTGACTGG197820.20694862815719056No Hit
AACGGAATCCCAAAAGCAGCTG196330.20538987041806303No Hit
ACCGGGTGCTGTAGGCTTT193550.20248158416653642No Hit
AGCTCGGTCTGAGGCCCCTCAGT193520.20245019978252712No Hit
CCCCCCACTGCTAAATTTGACTGGCT185990.1945727193961979No Hit
CGCGACCTCAGATCAGACGTGG176430.1845715623585741No Hit
TCAGGCTCAGTCCCCTCCCGA173100.18108789573354406No Hit
TATTGCACTTGTCCCGGCCTGTAG166440.17412056248348398No Hit
TGAGGTAGTAGGTTGTGTGGTT165450.17308487781117773No Hit
TACGCCTGTCTGAGCGTCGCT161790.1692559829620456No Hit
TATTGCACTTGTCCCGGCCTGTT155890.16308372077355393No Hit
TTAATGCTAATCGTGATAGGGGTT153440.16052066274612942No Hit
TTCAAGTAATCCAGGATAGGC149180.1560640802168117No Hit
TGAGGTAGTAGGTTGTATAGT148020.15485055070178622No Hit
CACTAGATTGTGAGCTCCTGGA144900.15158657476482112No Hit
TTCACAGTGGCTAAGTTCTGC139140.14556077303503942No Hit
CGCGACCTCAGATCAGACGTGGCG138360.14474477905079813No Hit
AGAGGTAGTAGGTTGCATAGTT135290.14153311042051517No Hit
AACCGGGCGGAAACACCA134460.14066480912959178No Hit
TTCAAGTAATCCAGGATAGGCTT133430.13958727861193984No Hit
CAAAGAATTCTCCTTTTGGGCT130230.13623961098428333No Hit
ACCCCCCACTGCTAAATTTGACTG126880.13273502143658045No Hit
TTCACAGTGGCTAAGTTCCG124430.13017196340915593No Hit
ACTGGACTTGGAGTCAGAAGGCT123930.1296488903423346No Hit
TTATAAAGCAATGAGACTGATT123180.1288642807421026No Hit
TGTAAACATCCTACACTCAGCT122140.12777628876311423No Hit
TGTCAGTTTGTCAAATACCCCA119360.12486800251158764No Hit
TAGCTTATCAGACTGATGTTGACA118880.12436585236743915No Hit
TGTCAGTTTGTCAAATACCCCAA118330.1237904719939357No Hit
ACTGGACTTGGAGTCAGAAGG118210.12366493445789858No Hit
TGAGGTAGTAAGTTGTATTGTT116480.12185510164669677No Hit
CACTAGATTGTGAGCTCCTGGAA115450.12077757112904486No Hit
TGAATACCGGGTGCTGTAGGCTTT114540.11982557814743003No Hit
ACGCCTGTCTGAGCGTCGCT113550.1187898934751238No Hit
ACTGGACTTGGAGTCAGAAGGCA112750.11795297656820966No Hit
ATCCCACCGCTGCCACCA109420.11446930994317961No Hit
GTCAAGTAATCCAGGATAGGCT108090.11307793558543487No Hit
TCCCACTGCTTCACTTGACTAG107590.11255486251861356No Hit
TAGCAGCACGTAAATATTGGC105020.10986626695515193No Hit
CAAAGTGCTGTTCGTGCAGGTAG103970.10876781351482714No Hit
TCGTTTCCCGGCCAATGCACCA103510.10828658629335151No Hit
ACCACAGGGTAGAACCACGGAC102800.10754382253846523No Hit
AGCAGCATTGTACAGGGCTATGT99710.10431123098550941No Hit
ATCACATTGCCAGGGATTTCC97220.10170632711273919No Hit
CTCCCACTGCTTCACTTGACTAGCCT96170.1006078736724144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)