FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6439485
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5857019.095463379447269No Hit
CCCCCCACTGCTAAATTTGACTG5836959.064311819966969No Hit
AACATTCAACGCTGTCGGTGAGT2796184.342241654418016No Hit
CCCCCACTGCTAAATTTGACTG2397623.723310171543221No Hit
AACATTCAACGCTGTCGGTGAG1791192.7815733711624455No Hit
TTCAAGTAATCCAGGATAGGCT1657972.574693473158179No Hit
TATTGCACTTGTCCCGGCCTGTAA1517292.3562287977998237No Hit
TAGCAGCACGTAAATATTGGCG1436462.230706337540968No Hit
AAGCTGCCAGTTGAAGAACTGT1292252.006759857348841No Hit
TATTGCACTTGTCCCGGCCTGTAT1108031.7206810793099139No Hit
TAGCTTATCAGACTGATGTTGAC1101641.7107579255173355No Hit
TAGCTTATCAGACTGATGTTGACT1062921.6506288934596478No Hit
TGAGGTAGTAGGTTGTATAGTT911901.4161070334040688No Hit
TAGCTTATCAGACTGATGTTGA624130.9692234705104522No Hit
TATTGCACTTGTCCCGGCCTGT594580.9233347076668399No Hit
TGAGGTAGTAGATTGTATAGTT586360.910569711708312No Hit
TGTAAACATCCTTGACTGGAAGCT505640.7852180725632563No Hit
TATTGCACTTGTCCCGGCCTGTA499600.7758384404964062No Hit
AGCAGCATTGTACAGGGCTATGA456180.7084106881217985No Hit
TGTAAACATCCCCGACTGGAAGCT435230.6758770305389328No Hit
ACTGGACTTGGAGTCAGAAGGC377310.5859319495270197No Hit
CACCCGGCTGTGTGCACATGTGC374370.5813663670309038No Hit
CATAAAGTAGAAAGCACTACT373150.5794718055869374No Hit
TTCAAGTAATTCAGGATAGGTT358970.557451411098869No Hit
CAACGGAATCCCAAAAGCAGCTGT305640.4746342292900752No Hit
CATTGCACTTGTCTCGGTCTGA289830.45008257647933025No Hit
GCCTGTCTGAGCGTCGCT277270.4305779111217745No Hit
CAACGGAATCCCAAAAGCAGCT261620.406274725385648No Hit
TCGTACGACTCTTAGCGG246590.3829343495636685No Hit
CCCCCACTGCTAAATTTGACTGGCTT243810.3786172341421713No Hit
CCCATAAAGTAGAAAGCACTAC221340.3437231393504294No Hit
TGAGGTAGTAGTTTGTACAGTT214400.33294587998885006No Hit
TTCTCACTACTGCACTTGACTAG210860.3274485459629147No Hit
TGATGTAGTAGGTTGTATAGTT202690.3147611959652053No Hit
AACATTCAACGCTGTCGGTGAGTT199810.3102887886220715No Hit
TTAATGCTAATCGTGATAGGGGTT197700.30701212907553943No Hit
CCCATAAAGTAGAAAGCACT175490.27252179328005266No Hit
TATTGCACTTGTCCCGGCCTGTAG175430.2724286181270707No Hit
CTTCTCACTACTGCACTTGACTAG172690.26817361947422813No Hit
CAACACCAGTCGATGGGCTGT171730.26668281702651686No Hit
CACTAGATTGTGAGCTCCTGGAA166470.2585144619484322No Hit
CACTAGATTGTGAGCTCCTGGA165820.25750506445779436No Hit
TCGTTTCCCGGCCAATGCACCA155880.2420690474471173No Hit
TAGCTTATCAGACTGATGTTGACA155520.24150999652922553No Hit
CAAAGAATTCTCCTTTTGGGCT152720.237161822723401No Hit
TGTCAGTTTGTCAAATACCCCAA141250.2193498393116841No Hit
TGTAAACATCCTACACTCTCAGCT139100.21601106299649742No Hit
TCCTGTCTGAGCGTCGCT136980.21271887425780167No Hit
TATTGCACTTGTCCCGGCCTGTAAT133430.2072060110397027No Hit
TATTGCACTTGTCCCGGCCTGTAAA130120.20206584843353156No Hit
AACGGAATCCCAAAAGCAGCTG128770.1999694074914376No Hit
ACCGGGTGCTGTAGGCTT127980.19874260131050853No Hit
TGATGTAGTAGATTGTATAGTT127520.1980282584709802No Hit
CAAAGAATTCTCCTTTTGGGCTT126360.19622687217999576No Hit
TGAGGTAGTAGTTTGTGCTGTT125390.19472054054012083No Hit
TGAGAACTGAATTCCATAGGCTGT124120.19274833313533615No Hit
CGCCTGTCTGAGCGTCGCT119490.18555831716356197No Hit
CCCCCCACTGCTAAATTTGACTGGCT115750.17975039929435352No Hit
CCCCCCACTGCTAAATTTGACTGG114950.17850806392126078No Hit
TTATAAAGCAATGAGACTGATT112460.1746412950725097No Hit
ACTGGACTTGGAGTCAGAAGGCA109860.17060370510995831No Hit
TGAGAACTGAATTCCATGGGTT109520.17007571257639392No Hit
ACCCCCCACTGCTAAATTTGACTG109470.16999806661557562No Hit
TAGCAGCACGTAAATATTGGC106380.16519954623700497No Hit
TTCAAGTAATCCAGGATAGGCTT102160.15864622714394086No Hit
TATTGCACTTGTCCCGGCCTGTT101920.15827352653201304No Hit
ACTGGACTTGGAGTCAGAAGGCT99460.1544533452597529No Hit
ACTTGACTTGGAGTCAGAAGGC96440.14976352922632788No Hit
TGTAAACATCCCCGACTGGAAGC95320.14802425970399807No Hit
TGTCAGTTTGTCAAATACCCCA94680.1470303914055239No Hit
ATGCACCTGGGCAAGGATTCTGA91960.14280645113700863No Hit
ACCGGGTGCTGTAGGCTTA89400.13883097794311192No Hit
ACCGGGTGCTGTAGGCTTT87990.136641361848036No Hit
CAACGGAATCCCAAAAGCAGCTGA83060.12898547011135209No Hit
CTCCCACTGCTTCACTTGACTAG82250.1277276055460957No Hit
AGCTCGGTCTGAGGCCCCTCAGT80870.12558457702751075No Hit
CCCGCCACTGCTAAATTTGACTG80160.12448200438389095No Hit
AACATTCAACGCTGTCGGTGA79940.12414036215629046No Hit
ATCCCGGACGAGCCCCCA78490.12188862929255989No Hit
AGCAGCATTGTACAGGGCTATGT78470.12185757090823256No Hit
TGAGGTAGTAGGTTGTGTGGTT74710.11601859465469677No Hit
CTCCCACTGCTTCACTTGACTAGCCT74180.11519554747002284No Hit
ACCACAGGGTAGAACCACGGAC73160.11361156986932962No Hit
CAAGGGAATCCCAAAAGCAGCTG71630.11123560346828977No Hit
ACTGGACTTGGAGTCAGAAGG71630.11123560346828977No Hit
TGAGGTAGTAAGTTGTATTGTT69770.1083471737258492No Hit
TGAGGTAGTAGGTTGTATAGT69250.10753965573333893No Hit
CAAAGTGCTGTTCGTGCAGGTAG68360.10615755763077325No Hit
TCAGTGCACTACAGAACTTTGT67450.10474440114388031No Hit
TATTGCACTTGTCCCGGCCTGTTT67310.10452699245358907No Hit
CGCGACCTCAGATCAGACGTGG66310.10297407323722316No Hit
TTCAAGTAATCCAGGATAGGC64830.10067575279700162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)