FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9623974
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6519936.774675409555346No Hit
CAACGGAATCCCAAAAGCAGCTG6195826.437901848030761No Hit
AACATTCAACGCTGTCGGTGAGT4190664.354396634903627No Hit
TTCAAGTAATCCAGGATAGGCT2961853.0775748147283024No Hit
AAGCTGCCAGTTGAAGAACTGT2919283.033341528146273No Hit
TAGCAGCACGTAAATATTGGCG2719082.8253193535227754No Hit
CCCCCACTGCTAAATTTGACTG2340992.432456696163144No Hit
AACATTCAACGCTGTCGGTGAG1978872.056188015470532No Hit
TAGCTTATCAGACTGATGTTGAC1671371.7366734365658096No Hit
TATTGCACTTGTCCCGGCCTGTAA1573761.6352496380393382No Hit
TAGCTTATCAGACTGATGTTGACT1462391.519528211526756No Hit
TATTGCACTTGTCCCGGCCTGTAT1181371.2275282539208854No Hit
TGTAAACATCCTTGACTGGAAGCT1075021.1170229678509107No Hit
TGAGGTAGTAGGTTGTATAGTT1029631.0698594987891696No Hit
CGCCTGTCTGAGCGTCGCT935060.9715944785386992No Hit
TATTGCACTTGTCCCGGCCTGT901590.9368167453486471No Hit
TAGCTTATCAGACTGATGTTGA823450.8556236747938014No Hit
AGCAGCATTGTACAGGGCTATGA724410.7527140035914478No Hit
CATAAAGTAGAAAGCACTACT699180.7264982220442407No Hit
TATTGCACTTGTCCCGGCCTGTA669750.6959183389315058No Hit
TGTAAACATCCCCGACTGGAAGCT646060.6713027279583258No Hit
TGAGGTAGTAGATTGTATAGTT601570.6250744235177692No Hit
ACTGGACTTGGAGTCAGAAGGC578760.6013731957297475No Hit
TTCAAGTAATTCAGGATAGGTT461850.47989531143787384No Hit
AACATTCAACGCTGTCGGTGAGTT430230.44703986108025645No Hit
CCCATAAAGTAGAAAGCACTAC428770.44552281625033485No Hit
CAACGGAATCCCAAAAGCAGCTGT392290.4076174769383209No Hit
TTCTCACTACTGCACTTGACTAG370720.3852046981839311No Hit
CCCCCACTGCTAAATTTGACTGGCTT356220.3701381570648466No Hit
TCGTACGACTCTTAGCGG329320.3421871256094416No Hit
CACCCGGCTGTGTGCACATGTGC300000.31172154039485145No Hit
GCCTGTCTGAGCGTCGCT298380.3100382440767192No Hit
CATTGCACTTGTCTCGGTCTGA294940.30646383708019165No Hit
TGTAAACATCCTACACTCTCAGCT288560.29983455898779443No Hit
GGAATACCGGGTGCTGTAGGCTTT287900.2991487715989258No Hit
TGAGGTAGTAGTTTGTACAGTT287540.29877470575045195No Hit
TTAATGCTAATCGTGATAGGGGTT286990.29820321625972807No Hit
TACGCCTGTCTGAGCGTCGCT279360.29027509841568566No Hit
TGTCAGTTTGTCAAATACCCCAA275370.28612920192843416No Hit
CTTCTCACTACTGCACTTGACTAG272330.28297042365243297No Hit
CTACGCCTGTCTGAGCGTCGCT262550.27280830143556084No Hit
ACCGGGTGCTGTAGGCTT233490.24261287488931285No Hit
CCCCCCACTGCTAAATTTGACTGGCT227410.23629531833731054No Hit
ACGCCTGTCTGAGCGTCGCT214950.22334848369291108No Hit
CAAAGAATTCTCCTTTTGGGCT213840.22219511399345013No Hit
TGATGTAGTAGGTTGTATAGTT213030.221353465834384No Hit
CCCATAAAGTAGAAAGCACT205850.2138929303009339No Hit
GGAATACCGGGTGCTGTAGGCTT201250.20911320001487949No Hit
CCCCCCACTGCTAAATTTGACTGG197360.2050712107077596No Hit
CAAAGAATTCTCCTTTTGGGCTT193170.20071749986024487No Hit
CAACACCAGTCGATGGGCTGT192400.1999174145732314No Hit
TAGCTTATCAGACTGATGTTGACA189400.19680019916928285No Hit
TATTGCACTTGTCCCGGCCTGTAG177820.18476774771004162No Hit
TTCAAGTAATCCAGGATAGGCTT173000.17975942162769765No Hit
AACCGGGCGGAAACACCA170630.17729682145857834No Hit
TGAGGTAGTAGTTTGTGCTGTT169520.1761434517591174No Hit
TGTCAGTTTGTCAAATACCCCA165050.1714988008072341No Hit
TTATAAAGCAATGAGACTGATT164830.17127020501094453No Hit
TGTAAACATCCCCGACTGGAAGC160760.16704118277958774No Hit
GCGTTCGCGCTTTCCCCTG160300.1665632097509823No Hit
TATTGCACTTGTCCCGGCCTGTT160030.1662826603646269No Hit
ACTGGACTTGGAGTCAGAAGGCA157480.1636330272712707No Hit
ATCCCGGACGAGCCCCCA151860.1577934437478738No Hit
ACCGGGTGCTGTAGGCTTA151360.15727390784721573No Hit
AGCTCGGTCTGAGGCCCCTCAGT150160.1560270216856363No Hit
ACCGGGTGCTGTAGGCTTT147060.15280589910155618No Hit
TTCACAGTGGCTAAGTTCCG143120.14871195620437047No Hit
TAGCAGCACGTAAATATTGGC143100.14869117476834415No Hit
CTCCCACTGCTTCACTTGACTAG137840.14322565709342108No Hit
CACTAGATTGTGAGCTCCTGGAA136950.1423008831902497No Hit
CACTAGATTGTGAGCTCCTGGA136860.14220736672813122No Hit
CAACGGAATCCCAAAAGCAGCT136290.141615095801381No Hit
ACCACAGGGTAGAACCACGGAC132770.13795756306074808No Hit
AACGGAATCCCAAAAGCAGCTG132510.13768740439240587No Hit
ACTTGACTTGGAGTCAGAAGGC132200.13736529213399787No Hit
TGAATACCGGGTGCTGTAGGCTTT131140.13626387602460274No Hit
TCCTGTCTGAGCGTCGCT130490.1355884793537472No Hit
AGCAGCATTGTACAGGGCTATGT129720.13478839406673376No Hit
ACCCCCCACTGCTAAATTTGACTG128030.13303236272250943No Hit
ACTGGACTTGGAGTCAGAAGGCT125920.13083992122173232No Hit
TGATGTAGTAGATTGTATAGTT125350.1302476502949821No Hit
TGTAAACATCCTACACTCAGCT123170.12798247376811284No Hit
CAAAGTGCTGTTCGTGCAGGTAG122300.12707848130096777No Hit
ACTGGACTTGGAGTCAGAAGG119940.1246262718498616No Hit
TGAGGTAGTAGGTTGTGTGGTT119820.12450158323370367No Hit
TCGTTTCCCGGCCAATGCACCA118710.1233482135342427No Hit
TTCAAGTAATCCAGGATAGGC118040.12265203542736088No Hit
TATTGCACTTGTCCCGGCCTGTAAT116170.12070897115889964No Hit
CCAAGTCCTTCTGATCGAGGCCC111800.11616822738714798No Hit
TAGCAGCACGTAAATATTGGCGT110910.11524345348397658No Hit
TATTGCACTTGTCCCGGCCTGTAAA107300.1114924042812252No Hit
AACATTCAACGCTGTCGGTGAGA105270.10938308852455336No Hit
AGCAGCATTGTACAGGGCTATG104530.1086141753915794No Hit
CCCCCACTGCTAAATTTGACTGGCT103720.10777252723251331No Hit
AACATTCAACGCTGTCGGTGA103540.10758549430827641No Hit
TGCAAGTAATCCAGGATAGGCT101800.10577750937398626No Hit
CTCCCACTGCTTCACTTGACTAGCCT97730.10154848714262943No Hit
GTCAGGATGGCCGAGCGGTCTAAGG97590.10140301709044518No Hit
CCTCGACACAAGGGTTTGT97550.10136145421839253No Hit
ATCACATTGCCAGGGATTTCCA96300.10006261446674732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)