FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6350812
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG65281710.279268225858363No Hit
CAACGGAATCCCAAAAGCAGCTG5441958.568904259801739No Hit
AACATTCAACGCTGTCGGTGAGT2450823.859065580905245No Hit
TAGCAGCACGTAAATATTGGCG2312443.6411721839663964No Hit
CCCCCACTGCTAAATTTGACTG1936193.048728257111059No Hit
TTCAAGTAATCCAGGATAGGCT1924613.030494368279206No Hit
AAGCTGCCAGTTGAAGAACTGT1647212.593699829250181No Hit
AACATTCAACGCTGTCGGTGAG1441112.2691743984863666No Hit
TATTGCACTTGTCCCGGCCTGTAA1391942.191751228031943No Hit
TATTGCACTTGTCCCGGCCTGTAT1177491.8540778722468874No Hit
TAGCTTATCAGACTGATGTTGAC823611.2968577876340852No Hit
TAGCTTATCAGACTGATGTTGACT783761.2341099059458853No Hit
TATTGCACTTGTCCCGGCCTGT649791.0231605029404114No Hit
TAGCTTATCAGACTGATGTTGA538810.8484111952928223No Hit
TGTAAACATCCTTGACTGGAAGCT516950.8139903999677521No Hit
TATTGCACTTGTCCCGGCCTGTA476910.7509433439377515No Hit
TGTAAACATCCCCGACTGGAAGCT435220.6852981949394817No Hit
TTCAAGTAATTCAGGATAGGTT413090.6504522571286947No Hit
TGAGGTAGTAGGTTGTATAGTT360320.5673605201980472No Hit
AGCAGCATTGTACAGGGCTATGA352380.5548581819143756No Hit
CATAAAGTAGAAAGCACTACT338870.5335853116105468No Hit
TTCTCACTACTGCACTTGACTAG333140.5245628433025572No Hit
CAACGGAATCCCAAAAGCAGCTGT314170.49469264717645556No Hit
ACTGGACTTGGAGTCAGAAGGC304390.4792930415827142No Hit
TGAGGTAGTAGATTGTATAGTT299680.47187666710965465No Hit
CTTCTCACTACTGCACTTGACTAG281570.4433606285306509No Hit
CACCCGGCTGTGTGCACATGTGC276920.4360387301655284No Hit
CCCCCCACTGCTAAATTTGACTGG242140.38127407959801046No Hit
CCCATAAAGTAGAAAGCACTAC206060.3244624466918561No Hit
CATTGCACTTGTCTCGGTCTGA185460.29202564963346417No Hit
CCCCCACTGCTAAATTTGACTGGCTT180540.28427860878262495No Hit
CTCCCACTGCTTCACTTGACTAG175920.27700394847147103No Hit
CAAAGAATTCTCCTTTTGGGCT168470.2652731650692856No Hit
ACCCCCCACTGCTAAATTTGACTG166450.2620924694354045No Hit
AACATTCAACGCTGTCGGTGAGTT165360.26037615347454784No Hit
TATTGCACTTGTCCCGGCCTGTAG162610.25604599852743243No Hit
TGTAAACATCCTACACTCTCAGCT157360.2477793390829393No Hit
CAACGGAATCCCAAAAGCAGCT153480.2416698841030092No Hit
CCCATAAAGTAGAAAGCACT146280.2303327511505615No Hit
CCCCCCACTGCTAAATTTGACTGGCT142190.22389262979285168No Hit
TAGCAGCACGTAAATATTGGC140070.22055447397907546No Hit
CAAAGAATTCTCCTTTTGGGCTT139910.22030253769124325No Hit
TGTAAACATCCCCGACTGGAAGC136760.21534254202454745No Hit
TAGCTTATCAGACTGATGTTGACA124280.19569151157363815No Hit
CGCGACCTCAGATCAGACGTGGCG123280.1941169097746871No Hit
TATTGCACTTGTCCCGGCCTGTAAT121930.19199119734610315No Hit
TGAGGTAGTAGTTTGTACAGTT121850.19186522920218704No Hit
CGCCTGTCTGAGCGTCGCT121630.19151881680641783No Hit
TATTGCACTTGTCCCGGCCTGTT117760.18542510784447722No Hit
CACTAGATTGTGAGCTCCTGGAA116190.18295298302012403No Hit
CACTAGATTGTGAGCTCCTGGA113980.1794731130444422No Hit
AGCTCGGTCTGAGGCCCCTCAGT112820.17764657495765895No Hit
AACGGAATCCCAAAAGCAGCTG112170.17662308378834077No Hit
TTCAAGTAATCCAGGATAGGCTT110820.17449737135975682No Hit
GGAATACCGGGTGCTGTAGGCTTT110470.17394626073012398No Hit
TATTGCACTTGTCCCGGCCTGTAAA103630.16317598442529868No Hit
TGAGAACTGAATTCCATAGGCTGT98450.1550195471067322No Hit
CTCCCACTGCTTCACTTGACTAGCCT93990.14799682308341044No Hit
TATTGCACTTGTCCCGGCCTGTTT89040.14020254417860267No Hit
CTACGCCTGTCTGAGCGTCGCT87630.1379823556420817No Hit
GGAATACCGGGTGCTGTAGGCTT86950.13691162641879495No Hit
TGAGGTAGTAGTTTGTGCTGTT86530.13625029366323552No Hit
ACTGGACTTGGAGTCAGAAGGCT85220.1341875653066096No Hit
ACTGGACTTGGAGTCAGAAGGCA82970.13064471125896973No Hit
CAACACCAGTCGATGGGCTGT82240.12949525194573544No Hit
TTTAAACATCCTTGACTGGAAGCT82010.1291330935319767No Hit
TGTCAGTTTGTCAAATACCCCA81410.12818833245260608No Hit
TGTCAGTTTGTCAAATACCCCAA81190.1278419200568368No Hit
CGCCCCACTGCTAAATTTGACTG78660.12385817750549064No Hit
CAACGGAATCCCAAAAGCAGCTGA77170.12151202082505357No Hit
TTCAAGTAATCCAGGATAGGC75400.11872497564091017No Hit
TCGTTTCCCGGCCAATGCACCA74540.11737081809381227No Hit
AACATTCAACGCTGTCGGTGA73820.11623710479856748No Hit
CCCCCACTGCTAAATTTGACTGG72530.11420586847792062No Hit
ACTTGACTTGGAGTCAGAAGGC71990.11335558350648704No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC70600.11116688700594506No Hit
TCGTACGACTCTTAGCGG66990.10548257451173174No Hit
AACATTCAACGCTGTCGGTGAGA65720.1034828302270639No Hit
TTTAAACATCCCCGACTGGAAGCT65090.1024908310937247No Hit
ATGCACCTGGGCAAGGATTCTGA64480.10153032399636457No Hit
CCCGCCACTGCTAAATTTGACTG64000.10077451513286804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)