FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8690131
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7943319.140610193333105No Hit
CCCCCCACTGCTAAATTTGACTG6662697.666961522214106No Hit
TAGCAGCACGTAAATATTGGCG3580014.119627195493371No Hit
TTCAAGTAATCCAGGATAGGCT2968813.4163006288397724No Hit
AACATTCAACGCTGTCGGTGAGT2697123.103658621486834No Hit
AAGCTGCCAGTTGAAGAACTGT2694903.101103999467902No Hit
CCCCCACTGCTAAATTTGACTG2390172.750441851797171No Hit
AACATTCAACGCTGTCGGTGAG1735611.9972196046296655No Hit
TATTGCACTTGTCCCGGCCTGTAA1310641.5081936049065314No Hit
TATTGCACTTGTCCCGGCCTGTAT1183161.3614984630266218No Hit
TATTGCACTTGTCCCGGCCTGT976791.1240221810235083No Hit
TAGCTTATCAGACTGATGTTGAC934171.0749780411825782No Hit
CATAAAGTAGAAAGCACTACT858860.988316516747561No Hit
TAGCTTATCAGACTGATGTTGA806760.9283634504474099No Hit
TAGCTTATCAGACTGATGTTGACT750170.8632436035774375No Hit
TGTAAACATCCTTGACTGGAAGCT694480.7991594142827076No Hit
TTCAAGTAATTCAGGATAGGTT641070.7376988908452589No Hit
TATTGCACTTGTCCCGGCCTGTA609300.7011401784391973No Hit
TTCTCACTACTGCACTTGACTAG507990.5845596573860624No Hit
TGAGGTAGTAGGTTGTATAGTT484060.5570226731910025No Hit
AGCAGCATTGTACAGGGCTATGA477940.5499802016793532No Hit
CAACGGAATCCCAAAAGCAGCTGT474680.5462288197956969No Hit
TGTAAACATCCCCGACTGGAAGCT454020.522454724790685No Hit
CCCATAAAGTAGAAAGCACTAC388860.4474731163431253No Hit
TGAGGTAGTAGATTGTATAGTT382580.4402465279292107No Hit
TTAATGCTAATCGTGATAGGGGTT372850.4290499188101997No Hit
CACCCGGCTGTGTGCACATGTGC352510.40564405760971844No Hit
CTTCTCACTACTGCACTTGACTAG335280.38581696869701965No Hit
CATTGCACTTGTCTCGGTCTGA330760.3806156662080238No Hit
ACTGGACTTGGAGTCAGAAGGC304650.35057008921959865No Hit
CCCATAAAGTAGAAAGCACT298840.3438843442060885No Hit
CTCCCACTGCTTCACTTGACTAG260110.29931654655148465No Hit
CAACGGAATCCCAAAAGCAGCT259150.2982118451378926No Hit
GGAATACCGGGTGCTGTAGGCTTT239050.2750821592908093No Hit
TGTAAACATCCTACACTCTCAGCT230040.26471407623199233No Hit
CCCCCCACTGCTAAATTTGACTGG221000.25431147125400066No Hit
TGAGAACTGAATTCCATGGGTT213620.24581907913701187No Hit
TAGCAGCACGTAAATATTGGC201880.23230950143329254No Hit
GGAATACCGGGTGCTGTAGGCTT191160.21997366898151477No Hit
CCCCCACTGCTAAATTTGACTGGCTT191050.21984708861120736No Hit
CGCCTGTCTGAGCGTCGCT182170.20962860053548102No Hit
AACATTCAACGCTGTCGGTGAGTT174400.20068742346922042No Hit
ATCCCGGACGAGCCCCCA169900.19550913559300775No Hit
TGTAAACATCCCCGACTGGAAGC168400.19378303963427018No Hit
TGAGGTAGTAGTTTGTACAGTT166900.1920569436755326No Hit
CAAAGAATTCTCCTTTTGGGCT165900.19090621303637426No Hit
TGTCAGTTTGTCAAATACCCCAA163630.18829405448548475No Hit
ACCCCCCACTGCTAAATTTGACTG163380.18800637182569516No Hit
TATTGCACTTGTCCCGGCCTGTT160780.18501447216388336No Hit
TGATGTAGTAGGTTGTATAGTT160520.1847152821977022No Hit
CACTAGATTGTGAGCTCCTGGA155160.1785473659718133No Hit
AACGGAATCCCAAAAGCAGCTG154080.17730457688152226No Hit
AGCTCGGTCTGAGGCCCCTCAGT152170.17510668136072977No Hit
TATTGCACTTGTCCCGGCCTGTAG149390.1719076501838695No Hit
CAACACCAGTCGATGGGCTGT147780.17005497385482451No Hit
TGTCAGTTTGTCAAATACCCCA143900.16559013897489003No Hit
CCCCCCACTGCTAAATTTGACTGGCT142310.1637604772586282No Hit
TTCAAGTAATCCAGGATAGGCTT137860.15863972591437345No Hit
TGAATACCGGGTGCTGTAGGCTTT136560.15714377608346755No Hit
ACCGGGTGCTGTAGGCTT136220.15675252766615372No Hit
CTACGCCTGTCTGAGCGTCGCT134260.1544970956134033No Hit
TTCAAGTAATCCAGGATAGGC134040.15424393487278845No Hit
CAAAGAATTCTCCTTTTGGGCTT133960.1541518764216558No Hit
CGCGACCTCAGATCAGACGTGGCG132140.15205754665838755No Hit
TTCACAGTGGCTAAGTTCCG129460.1489735885454431No Hit
TGAGGTAGTAGTTTGTGCTGTT128200.14752366794010355No Hit
CACTAGATTGTGAGCTCCTGGAA124950.14378379336283884No Hit
TGATGTAGTAGATTGTATAGTT124670.1434615887838745No Hit
TCGTTTCCCGGCCAATGCACCA122620.1411025909735998No Hit
TAGCTTATCAGACTGATGTTGACA116690.13427875828339067No Hit
ACCGGGTGCTGTAGGCTTA114260.1314824828302358No Hit
TCGTACGACTCTTAGCGG112850.1298599526290225No Hit
TGAATACCGGGTGCTGTAGGCTT111890.12875525121543047No Hit
TGAGAACTGAATTCCATAGGCTGT111470.12827194434698394No Hit
CCCATAAAGTAGAAAGCACTA110040.1266263995329875No Hit
TTCACAGTGGCTAAGTTCCGC108940.12536059582991327No Hit
ACTTGACTTGGAGTCAGAAGGC108420.1247622158975509No Hit
TTCACAGTGGCTAAGTTCTGC108160.12446302593136974No Hit
AACCGGGCGGAAACACCA107320.12349641219447671No Hit
CAACGGAATCCCAAAAGCAGCTGA104420.12015929334091742No Hit
CTCCCACTGCTTCACTTGACTAGCCT101760.11709834984075614No Hit
CAAAGTGCTGTTCGTGCAGGT97890.11264502226721322No Hit
ACCGGGTGCTGTAGGCTTT97630.11234583230103204No Hit
TATTGCACTTGTCCCGGCCTGA95020.10934242533282869No Hit
AATGACACGATCACTCCCGTTG94590.1088476111579906No Hit
TATTGCACTTGTCCCGGCCTGTTT90810.10449784934197194No Hit
TATTGCACTTGTCCCGGCCTGTAAT90210.10380741095847691No Hit
TTATAAAGCAATGAGACTGATT89170.1026106510937522No Hit
AGCAGCATTGTACAGGGCTATGT88550.10189719809747402No Hit
ATCCCACCGCTGCCACCA87160.10029768250904388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)