FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8363230
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG86345710.324444024617282No Hit
CAACGGAATCCCAAAAGCAGCTG7509498.979174314230267No Hit
CCCCCACTGCTAAATTTGACTG3693034.415793897812209No Hit
AACATTCAACGCTGTCGGTGAGT2731083.2655804037435296No Hit
TTCAAGTAATCCAGGATAGGCT2210122.6426631815698003No Hit
AACATTCAACGCTGTCGGTGAG2093182.5028368226151856No Hit
TAGCAGCACGTAAATATTGGCG1761602.1063632113429858No Hit
TATTGCACTTGTCCCGGCCTGTAA1712262.047366866629281No Hit
AAGCTGCCAGTTGAAGAACTGT1698852.0313323919107806No Hit
TATTGCACTTGTCCCGGCCTGTAT1378981.64886054789836No Hit
TAGCTTATCAGACTGATGTTGAC1316851.574571068833453No Hit
TAGCTTATCAGACTGATGTTGACT1224631.464302667749183No Hit
TAGCTTATCAGACTGATGTTGA890961.065330022013026No Hit
TATTGCACTTGTCCCGGCCTGT829970.9924036526557323No Hit
GCCTGTCTGAGCGTCGCT735470.8794090321562362No Hit
TATTGCACTTGTCCCGGCCTGTA713760.8534501621980981No Hit
TGAGGTAGTAGGTTGTATAGTT563820.6741653643388977No Hit
AGCAGCATTGTACAGGGCTATGA559820.669382523259554No Hit
TGTAAACATCCTTGACTGGAAGCT555680.6644322827424333No Hit
TGTAAACATCCCCGACTGGAAGCT517010.6181941666078776No Hit
CATAAAGTAGAAAGCACTACT503210.6016933648841417No Hit
ACTGGACTTGGAGTCAGAAGGC455320.5444308000616986No Hit
CACCCGGCTGTGTGCACATGTGC443360.5301301052344608No Hit
TGAGGTAGTAGATTGTATAGTT423850.5068017978699617No Hit
CATTGCACTTGTCTCGGTCTGA385770.4612691507946093No Hit
CAACGGAATCCCAAAAGCAGCTGT359480.42983392780062246No Hit
TTCAAGTAATTCAGGATAGGTT357900.4279447055742817No Hit
TCGTACGACTCTTAGCGG313170.37446058520452025No Hit
CAACGGAATCCCAAAAGCAGCT311070.3719495936378648No Hit
TCCTGTCTGAGCGTCGCT303080.36239586858187567No Hit
TTAATGCTAATCGTGATAGGGGTT298080.356417317232696No Hit
CCCATAAAGTAGAAAGCACTAC266930.31917094232730653No Hit
CAACACCAGTCGATGGGCTGT253190.30274188321976075No Hit
CCCATAAAGTAGAAAGCACT244620.29249464620726684No Hit
TATTGCACTTGTCCCGGCCTGTAG207280.24784682473159292No Hit
TGAGAACTGAATTCCATAGGCTGT205780.24605325932683902No Hit
ACCGGGTGCTGTAGGCTT204920.24502494849478013No Hit
CACTAGATTGTGAGCTCCTGGA202650.24231068618225257No Hit
TCGTTTCCCGGCCAATGCACCA188880.2258457557666117No Hit
CCCCCACTGCTAAATTTGACTGGCTT186470.2229640940163071No Hit
CACTAGATTGTGAGCTCCTGGAA182110.2177507972398224No Hit
TAGCTTATCAGACTGATGTTGACA180080.21532350539205544No Hit
AACATTCAACGCTGTCGGTGAGTT179460.2145821650247572No Hit
TTCTCACTACTGCACTTGACTAG178520.21345819737111138No Hit
ACCCCCCACTGCTAAATTTGACTG176270.2107678492639805No Hit
AACGGAATCCCAAAAGCAGCTG169490.2026609336344929No Hit
TGTAAACATCCTACACTCTCAGCT167660.20047278384069311No Hit
CAAAGAATTCTCCTTTTGGGCT160660.19210281195184156No Hit
TGAGAACTGAATTCCATGGGTT159600.19083535906581547No Hit
CGCCTGTCTGAGCGTCGCT154170.18434265230060634No Hit
TGAGGTAGTAGTTTGTACAGTT149970.17932066916729542No Hit
TATTGCACTTGTCCCGGCCTGTT148340.17737166142746283No Hit
CTTCTCACTACTGCACTTGACTAG147580.17646292162238753No Hit
TATTGCACTTGTCCCGGCCTGTAAT146600.17529112555794832No Hit
TAGCAGCACGTAAATATTGGC146300.1749324124769975No Hit
TGAGGTAGTAGTTTGTGCTGTT139730.16707659600417543No Hit
ACTGGACTTGGAGTCAGAAGGCT136000.16261659669768738No Hit
ACCGGGTGCTGTAGGCTTT133560.15969906363928768No Hit
TATTGCACTTGTCCCGGCCTGTAAA131680.15745112833199612No Hit
ACTGGACTTGGAGTCAGAAGGCA126730.15153236249630825No Hit
CAAAGAATTCTCCTTTTGGGCTT126470.15122147782615092No Hit
GCCCCCACTGCTAAATTTGACTG125900.15053992297234442No Hit
TTCAAGTAATCCAGGATAGGCTT123950.14820828794616436No Hit
ACCGGGTGCTGTAGGCTTA122710.14672560721156777No Hit
CTCCCACTGCTTCACTTGACTAG122340.14628319441172846No Hit
CCCCCCACTGCTAAATTTGACTGG122000.14587665291998425No Hit
TCAGTGCACTACAGAACTTTGT120590.1441907014395156No Hit
AGCAGCATTGTACAGGGCTATGT120120.14362871761269272No Hit
TTATAAAGCAATGAGACTGATT118800.14205038005650927No Hit
TGATGTAGTAGGTTGTATAGTT115340.13791322252287694No Hit
CGCGACCTCAGATCAGACGTGGCG113300.13547397357241162No Hit
TGTAAACATCCCCGACTGGAAGC112910.13500764656717562No Hit
CCCGCCACTGCTAAATTTGACTG105470.12611156215959624No Hit
CTCCCACTGCTTCACTTGACTAGCCT102950.12309837227960968No Hit
TCAGTGCACTACAGAACTTTGTC102550.1226200881716753No Hit
ACTTGACTTGGAGTCAGAAGGC102040.12201027593405898No Hit
ACCACAGGGTAGAACCACGGAC101640.12153199182612459No Hit
TACCACCCTGAACGCGCCCGA94640.11316201993727304No Hit
ACTGGACTTGGAGTCAGAAGG92260.1103162294950635No Hit
CCCCCCACTGCTAAATTTGACTGGCT91190.10903681950633906No Hit
ATGCACCTGGGCAAGGATTCTGA88700.10605950093444758No Hit
CAACGGAATCCCAAAAGCAGCTGA88290.10556925972381485No Hit
TTCACAGTGGCTAAGTTCCGC88130.10537794608064109No Hit
CGCGACCTCAGATCAGACGTGG86850.1038474369352511No Hit
TGATGTAGTAGATTGTATAGTT85110.10176690106573658No Hit
TTCAAGTAATCCAGGATAGGC84730.1013125311631989No Hit
AGCAGCATTGTACAGGGCTATG84520.10106143200653336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)