FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8487943
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG110394713.006060478964102No Hit
CAACGGAATCCCAAAAGCAGCTG7456108.78434268467637No Hit
CCCCCACTGCTAAATTTGACTG3560264.194490938499469No Hit
TTCAAGTAATCCAGGATAGGCT3428424.039164730488883No Hit
AACATTCAACGCTGTCGGTGAGT2619293.08589489821032No Hit
TAGCAGCACGTAAATATTGGCG2533082.9843272981451454No Hit
AAGCTGCCAGTTGAAGAACTGT2032352.3943963808428026No Hit
AACATTCAACGCTGTCGGTGAG1755372.0680746795778435No Hit
TATTGCACTTGTCCCGGCCTGTAA1616701.9047017634307866No Hit
TATTGCACTTGTCCCGGCCTGTAT1385031.63176166475199No Hit
TAGCTTATCAGACTGATGTTGACT872601.0280464890021057No Hit
TAGCTTATCAGACTGATGTTGAC842400.9924666082229817No Hit
TATTGCACTTGTCCCGGCCTGT799660.9421128299282877No Hit
GGAATACCGGGTGCTGTAGGCTTT758060.8931021332259182No Hit
TGTAAACATCCTTGACTGGAAGCT722780.8515372923687163No Hit
TAGCTTATCAGACTGATGTTGA658240.7755000239751846No Hit
TGAGGTAGTAGGTTGTATAGTT645680.7607025636246615No Hit
TATTGCACTTGTCCCGGCCTGTA636150.7494748727695273No Hit
TTAATGCTAATCGTGATAGGGGTT565180.665862152938586No Hit
TTCTCACTACTGCACTTGACTAG559240.6588639909575265No Hit
GGAATACCGGGTGCTGTAGGCTT555170.6540689540445783No Hit
TGTAAACATCCCCGACTGGAAGCT512510.6038094270896965No Hit
CATAAAGTAGAAAGCACTACT487720.5746032931653758No Hit
TTCAAGTAATTCAGGATAGGTT478240.5634345093976244No Hit
TGAGGTAGTAGATTGTATAGTT469550.5531964576105188No Hit
CAACGGAATCCCAAAAGCAGCTGT420140.4949844738589786No Hit
AGCAGCATTGTACAGGGCTATGA406990.4794919098773402No Hit
ACTGGACTTGGAGTCAGAAGGC386710.45559919523493503No Hit
CTTCTCACTACTGCACTTGACTAG369560.4353940642626841No Hit
TGAGAACTGAATTCCATGGGTT344820.40624683742574613No Hit
CACCCGGCTGTGTGCACATGTGC311740.3672739084133812No Hit
CCCCCACTGCTAAATTTGACTGGCTT304390.3586145665681308No Hit
CCCATAAAGTAGAAAGCACTAC277920.32742915450775295No Hit
CATTGCACTTGTCTCGGTCTGA254690.3000609217097711No Hit
CAAAGAATTCTCCTTTTGGGCT244060.2875372749322186No Hit
ACCCCCCACTGCTAAATTTGACTG236400.2785127091451957No Hit
CAACGGAATCCCAAAAGCAGCT233330.27489581397990065No Hit
CCCCCCACTGCTAAATTTGACTGG230750.27185620827095563No Hit
CTCCCACTGCTTCACTTGACTAG228260.2689226353192994No Hit
TGAGAACTGAATTCCATAGGCTGT225970.26622469071717375No Hit
AACGGAATCCCAAAAGCAGCTG191000.2250250738017444No Hit
TGAGGTAGTAGTTTGTACAGTT188320.22186765391803406No Hit
TATTGCACTTGTCCCGGCCTGTAG186500.2197234359373054No Hit
AACATTCAACGCTGTCGGTGAGTT182690.21523471587874707No Hit
CCCCCCACTGCTAAATTTGACTGGCT180700.21289021380091738No Hit
AGCTCGGTCTGAGGCCCCTCAGT180570.21273705537372248No Hit
CCCATAAAGTAGAAAGCACT179280.21121725251924994No Hit
TGTAAACATCCTACACTCTCAGCT179120.21102874983962547No Hit
ACCGGGTGCTGTAGGCTT166820.19653760634349218No Hit
ACCGGGTGCTGTAGGCTTA162360.1912830941489593No Hit
CACTAGATTGTGAGCTCCTGGA158240.18642915014862846No Hit
TATTGCACTTGTCCCGGCCTGTT156430.18429671358537633No Hit
TGTAAACATCCCCGACTGGAAGC156270.18410821090575183No Hit
TAGCAGCACGTAAATATTGGC154830.18241168678913136No Hit
TGAGGTAGTAGTTTGTGCTGTT153020.1802792502258792No Hit
CACTAGATTGTGAGCTCCTGGAA151540.17853560043935263No Hit
CAAAGAATTCTCCTTTTGGGCTT143770.16938143906008793No Hit
TGTCAGTTTGTCAAATACCCCAA143610.16919293638046345No Hit
CTCCCACTGCTTCACTTGACTAGCCT141710.16695446705992253No Hit
GGGAATACCGGGTGCTGTAGGCTT137670.16219477439940394No Hit
GGAATACCGGGTGCTGTAGGCTTA134900.15893132175840485No Hit
TCGTACGACTCTTAGCGG134180.1580830597000946No Hit
TTCAAGTAATCCAGGATAGGCTT131150.15451329020470567No Hit
TCGTTTCCCGGCCAATGCACCA127860.15063720385492693No Hit
ACTGGACTTGGAGTCAGAAGGCT127330.15001278872867077No Hit
ACCGGGTGCTGTAGGCTTT124360.14651370773814104No Hit
CGCGACCTCAGATCAGACGTGGCG124040.14613670237889204No Hit
CAACACCAGTCGATGGGCTGT123900.14597176253422062No Hit
TTCAAGTAATCCAGGATAGGC123550.14555941292254201No Hit
TATTGCACTTGTCCCGGCCTGTAAA122190.14395714014573377No Hit
TATTGCACTTGTCCCGGCCTGTAAT121700.14337985068938375No Hit
TTCACAGTGGCTAAGTTCCG121050.14261405855340922No Hit
ACTGGACTTGGAGTCAGAAGGCA118950.1401399608833377No Hit
TAGCTTATCAGACTGATGTTGACA115970.13662909847533142No Hit
GGGAATACCGGGTGCTGTAGGCTTT112200.13218750408667918No Hit
GACCCGCGGGCGCTCTCCAGTCCT106930.1259786970765473No Hit
TATTGCACTTGTCCCGGCCTGTTT106020.12490658808618296No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC104950.12364597641619413No Hit
TGTCAGTTTGTCAAATACCCCA104230.12279771435788388No Hit
CAACGGAATCCCAAAAGCAGCTGA99690.11744895082353875No Hit
ACTGGACTTGGAGTCAGAAGG94400.11121658097845379No Hit
GAATACCGGGTGCTGTAGGCTTT94050.1108042313667752No Hit
TTCACAGTGGCTAAGTTCTGC88960.10480748987122086No Hit
GGAATACCGGGTGCTGTAGGCT88150.10385319505562185No Hit
TTAATGCTAATCGTGATAGGGGT87700.10332303126917794No Hit
AATGCACCCGGGCAAGGATTCT86450.10185035408461156No Hit
AACATTCAACGCTGTCGGTGA85100.10025986272527984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)