FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7550697
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG82181210.883922371669794No Hit
CAACGGAATCCCAAAAGCAGCTG5463687.23599424000195No Hit
TAGCAGCACGTAAATATTGGCG2918473.8651663548411492No Hit
TTCAAGTAATCCAGGATAGGCT2530543.3513992151982794No Hit
CCCCCACTGCTAAATTTGACTG2472813.2749426973430396No Hit
AACATTCAACGCTGTCGGTGAGT2226472.9486946701741577No Hit
AAGCTGCCAGTTGAAGAACTGT2194762.906698547167235No Hit
TAGCTTATCAGACTGATGTTGAC1626692.1543574056805617No Hit
TAGCTTATCAGACTGATGTTGACT1316361.7433622353009266No Hit
TATTGCACTTGTCCCGGCCTGTAA1185241.5697093923911924No Hit
AACATTCAACGCTGTCGGTGAG1094161.4490847666116122No Hit
TGTAAACATCCTTGACTGGAAGCT993711.3160506904197056No Hit
TAGCTTATCAGACTGATGTTGA956341.2665585706855937No Hit
TATTGCACTTGTCCCGGCCTGTAT950701.2590890615793482No Hit
AGCAGCATTGTACAGGGCTATGA635470.8416044240684005No Hit
TGTAAACATCCCCGACTGGAAGCT547200.7247013090314709No Hit
CATAAAGTAGAAAGCACTACT511000.6767587151225907No Hit
TATTGCACTTGTCCCGGCCTGT493630.6537542163326114No Hit
TATTGCACTTGTCCCGGCCTGTA468990.6211214673294398No Hit
TTCAAGTAATTCAGGATAGGTT428170.567060232982465No Hit
ACTGGACTTGGAGTCAGAAGGC413640.5478169763665527No Hit
TGAGGTAGTAGGTTGTATAGTT386640.5120586880919734No Hit
CAACGGAATCCCAAAAGCAGCTGT362830.4805251753579835No Hit
CCCATAAAGTAGAAAGCACTAC352460.4667913438984507No Hit
TGTAAACATCCTACACTCTCAGCT331410.438913122854751No Hit
CCCCCACTGCTAAATTTGACTGGCTT330620.43786686182745777No Hit
TTCTCACTACTGCACTTGACTAG316970.41978906053308723No Hit
CTTCTCACTACTGCACTTGACTAG300770.39833408756833977No Hit
TGAGGTAGTAGATTGTATAGTT269300.35665581601274693No Hit
CAAAGAATTCTCCTTTTGGGCT263730.34927901357980595No Hit
CAACGGAATCCCAAAAGCAGCT245030.32451308799704187No Hit
TAGCTTATCAGACTGATGTTGACA229480.3039189627129787No Hit
CCCCCCACTGCTAAATTTGACTGGCT219510.2907148836723285No Hit
AACATTCAACGCTGTCGGTGAGTT216890.28724500532864716No Hit
CATTGCACTTGTCTCGGTCTGA204360.27065051080714797No Hit
CAAAGAATTCTCCTTTTGGGCTT196490.26022763196563176No Hit
CCCCCCACTGCTAAATTTGACTGG188280.2493544635680653No Hit
CACCCGGCTGTGTGCACATGTGC171660.22734325056349103No Hit
TTCAAGTAATCCAGGATAGGCTT164550.21792690131785183No Hit
ACCCCCCACTGCTAAATTTGACTG160110.21204664946825438No Hit
CTCCCACTGCTTCACTTGACTAG156540.2073186091297267No Hit
TGTAAACATCCCCGACTGGAAGC155620.2061001785662966No Hit
CCCATAAAGTAGAAAGCACT150490.19930610379412655No Hit
TAGCAGCACGTAAATATTGGC150290.19904122758468523No Hit
CTCCCACTGCTTCACTTGACTAGCCT142020.18808859632428637No Hit
CACTAGATTGTGAGCTCCTGGAA138610.18357245695331173No Hit
TATTGCACTTGTCCCGGCCTGTAG127420.16875263303506946No Hit
CACTAGATTGTGAGCTCCTGGA126320.16729581388314219No Hit
ACTGGACTTGGAGTCAGAAGGCA122460.16218370304092458No Hit
TTCACAGTGGCTAAGTTCCG121950.16150826870684917No Hit
TCGTTTCCCGGCCAATGCACCA121280.16062093340522074No Hit
AGCTCGGTCTGAGGCCCCTCAGT119820.15868733707629906No Hit
AGCAGCATTGTACAGGGCTATGT118710.15721727411389969No Hit
CCCGCCACTGCTAAATTTGACTG116010.15364144528644177No Hit
ATCACATTGCCAGGGATTTCCA114390.15149594798996702No Hit
CGCGACCTCAGATCAGACGTGGCG111710.14794660678345323No Hit
GTCAGGATGGCCGAGCGGTCTAAGG109330.14479457989110142No Hit
TATTGCACTTGTCCCGGCCTGTT108020.14305964071926075No Hit
TGTAAACATCCTACACTCAGCT107340.14215906160716024No Hit
TAGCAGCACGTAAATATTGGCGT106130.1405565605400402No Hit
TGAGGTAGTAGTTTGTACAGTT104230.1380402365503476No Hit
ACTTGACTTGGAGTCAGAAGGC104120.13789455463515488No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC103020.13643773548322757No Hit
TTCAAGTAATCCAGGATAGGC99980.1324116170997194No Hit
TATTGCACTTGTCCCGGCCTGTAAA98900.13098128556873623No Hit
AACGGAATCCCAAAAGCAGCTG98480.13042504552890943No Hit
CGCCTGTCTGAGCGTCGCT96380.12764384532977552No Hit
TGTCAGTTTGTCAAATACCCCA95880.1269816548061722No Hit
TCGTACGACTCTTAGCGG95390.12633270809304095No Hit
TATTGCACTTGTCCCGGCCTGTAAT95030.12585593091604655No Hit
TTATAAAGCAATGAGACTGATT94010.12450506224789579No Hit
TGAGGTAGTAGTTTGTGCTGTT92280.12221388303622831No Hit
CTACGCCTGTCTGAGCGTCGCT92150.12204171350009144No Hit
ACTGGACTTGGAGTCAGAAGGCT88610.11735340459297997No Hit
TGTAAACATCCTTGACTGGAAGC88210.11682365217409732No Hit
TGATGTAGTAGGTTGTATAGTT86240.11421462151110023No Hit
CAACGGAATCCCAAAAGCAGCTGA84470.11187046705754448No Hit
ATCACATTGCCAGGGATTTCC84140.1114334213119663No Hit
ACTGGACTTGGAGTCAGAAGG83970.11120827653394118No Hit
GCCTGTCTGAGCGTCGCT83820.11100961937686017No Hit
TTAATGCTAATCGTGATAGGGGTT81180.10751325341223465No Hit
ATGCACCTGGGCAAGGATTCTGA80780.10698350099335201No Hit
CAGTGCAATAGTATTGTCAAAGC79920.10584453329275431No Hit
CCCCCACTGCTAAATTTGACTGGCT79280.10499692942254205No Hit
AGCAGCATTGTACAGGGCTATG79200.10489097893876553No Hit
TCAGTGCACTACAGAACTTTGT77720.1029308949888997No Hit
CAAAGTGCTGTTCGTGCAGGTAG77590.10275872545276284No Hit
ACCACAGGGTAGAACCACGGAC76860.101791927288302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)