FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7805964
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG105412113.504046393244959No Hit
CAACGGAATCCCAAAAGCAGCTG7581709.712701723963882No Hit
CCCCCACTGCTAAATTTGACTG3447574.41658454996718No Hit
TTCAAGTAATCCAGGATAGGCT2333302.9891247256584834No Hit
TAGCAGCACGTAAATATTGGCG2305442.9534340665675627No Hit
GCCTGTCTGAGCGTCGCT1938132.4828836002830657No Hit
AAGCTGCCAGTTGAAGAACTGT1901692.4362013455352853No Hit
AACATTCAACGCTGTCGGTGAGT1838872.3557244178938053No Hit
AACATTCAACGCTGTCGGTGAG1231061.5770761945609792No Hit
TAGCTTATCAGACTGATGTTGAC1117751.4319179540156732No Hit
TATTGCACTTGTCCCGGCCTGTAA851981.0914475137215596No Hit
TAGCTTATCAGACTGATGTTGACT817281.0469943238272685No Hit
TGAGGTAGTAGGTTGTATAGTT790361.0125078721859337No Hit
TCCTGTCTGAGCGTCGCT775010.9928434207485456No Hit
TATTGCACTTGTCCCGGCCTGTAT753260.9649801100799337No Hit
TGTAAACATCCTTGACTGGAAGCT597420.7653378877996363No Hit
AACCGGGCGGAAACACCA594470.7615587261227441No Hit
TATTGCACTTGTCCCGGCCTGT558320.7152479821838789No Hit
TAGCTTATCAGACTGATGTTGA545950.6994011245760293No Hit
CAACGGAATCCCAAAAGCAGCTGT454490.582234301874823No Hit
AGCAGCATTGTACAGGGCTATGA437980.5610838072017754No Hit
TATTGCACTTGTCCCGGCCTGTA395600.5067919862300159No Hit
ACTGGACTTGGAGTCAGAAGGC386990.49576195842051024No Hit
CATAAAGTAGAAAGCACTACT384790.4929436005597771No Hit
TGAGGTAGTAGATTGTATAGTT378190.48448852697757766No Hit
TTCAAGTAATTCAGGATAGGTT368260.47176748445163214No Hit
CAACGGAATCCCAAAAGCAGCT356490.4566892698967097No Hit
TTCTCACTACTGCACTTGACTAG347990.4458001599802408No Hit
CCCCCACTGCTAAATTTGACTGGCTT341520.4375116257261755No Hit
TGTAAACATCCCCGACTGGAAGCT325550.41705290980076265No Hit
CCCATAAAGTAGAAAGCACTAC318810.4084184861728801No Hit
CACCCGGCTGTGTGCACATGTGC302650.38771636661404024No Hit
CTCCCACTGCTTCACTTGACTAG270350.346337748931458No Hit
CATTGCACTTGTCTCGGTCTGA267060.3421230228579071No Hit
CTTCTCACTACTGCACTTGACTAG253520.3247773112968494No Hit
CCCCCCACTGCTAAATTTGACTGG234400.3002832193435686No Hit
CCCCCCACTGCTAAATTTGACTGGCT215980.27668587761870284No Hit
TCGTACGACTCTTAGCGG202170.2589942766838279No Hit
AACGGAATCCCAAAAGCAGCTG197380.2528579429779589No Hit
ACCCCCCACTGCTAAATTTGACTG192940.24716998438629748No Hit
TGTAAACATCCTACACTCTCAGCT185190.2372416782859875No Hit
CGCGACCTCAGATCAGACGTGG178180.22826136528428775No Hit
CCCATAAAGTAGAAAGCACT168460.21580934782686675No Hit
TCAGTGCACTACAGAACTTTGT163000.208814696045229No Hit
CTCCCACTGCTTCACTTGACTAGCCT153590.1967598108318204No Hit
CGCCTGTCTGAGCGTCGCT153120.19615770710702743No Hit
TAGCAGCACGTAAATATTGGC152050.19478696032930717No Hit
TCAGTGCACTACAGAACTTTGTC150920.19333934924629426No Hit
TAGCTTATCAGACTGATGTTGACA136790.17523780534985814No Hit
CAAAGAATTCTCCTTTTGGGCT135710.17385424785458914No Hit
CACTAGATTGTGAGCTCCTGGA133690.17126648290973415No Hit
AACATTCAACGCTGTCGGTGAGTT127860.1637978345787913No Hit
TTCAAGTAATCCAGGATAGGCTT127110.16283703076263228No Hit
CGCGACCTCAGATCAGACGTGGCG126270.16176093048853415No Hit
AGCTCGGTCTGAGGCCCCTCAGT124870.15996743003170397No Hit
TGAGGTAGTAGTTTGTACAGTT120680.15459973937876217No Hit
CACTAGATTGTGAGCTCCTGGAA119210.1527165638990905No Hit
TGAGGTAGTAGTTTGTGCTGTT111770.1431853900427929No Hit
CAACGGAATCCCAAAAGCAGCTGA108190.13859915316032717No Hit
CAACACCAGTCGATGGGCTGT106940.13699781346672876No Hit
TTCAAGTAATCCAGGATAGGC104850.13432037349903228No Hit
CCCCCCACTGCTAAATTTGACTGTA103650.13278308739317785No Hit
GGAATACCGGGTGCTGTAGGCTTT101640.13020813316587165No Hit
TGTCAGTTTGTCAAATACCCCAA101320.12979819020431044No Hit
CCCCCCACTGCTAAATTTGACTGT101080.12949073298313957No Hit
TATTGCACTTGTCCCGGCCTGTT99420.1273641538700409No Hit
CAAAGAATTCTCCTTTTGGGCTT97500.12490449610067379No Hit
AATGCACCCGGGCAAGGATTCT97130.12443049955136867No Hit
TATTGCACTTGTCCCGGCCTGTAG96190.1232262921017827No Hit
CCCCCACTGCTAAATTTGACTGGCT95340.12213738111013579No Hit
TGTAAACATCCCCGACTGGAAGC95300.12208613823994063No Hit
AACTTAACTTGACCGCTCTGACCA93620.1199339376917444No Hit
TCGTTTCCCGGCCAATGCACCA93230.11943431970734171No Hit
TTCACAGTGGCTAAGTTCTGC93170.119357455402049No Hit
CTACGCCTGTCTGAGCGTCGCT92050.11792265503658483No Hit
TGTCAGTTTGTCAAATACCCCA91410.11710276911346248No Hit
TGAGGTAGTAGGTTGTGTGGTT90130.11546299726721773No Hit
CCCTGTCTGAGCGTCGCT84000.10761002740981127No Hit
CAAAGTGCTGTTCGTGCAGGTAG81940.10497101959476114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)