FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8272510
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG106833212.914242472961652No Hit
CAACGGAATCCCAAAAGCAGCTG5553996.713790614940327No Hit
CCCCCACTGCTAAATTTGACTG3069383.710337007752182No Hit
AACATTCAACGCTGTCGGTGAGT2834633.426565818596774No Hit
AAGCTGCCAGTTGAAGAACTGT2351192.8421724482653996No Hit
TTCAAGTAATCCAGGATAGGCT2232032.6981291047094533No Hit
TAGCAGCACGTAAATATTGGCG2138392.58493492301611No Hit
AACATTCAACGCTGTCGGTGAG1382861.6716329143150026No Hit
GCCTGTCTGAGCGTCGCT1296461.5671906108303282No Hit
TAGCTTATCAGACTGATGTTGAC1149871.3899892535639122No Hit
TATTGCACTTGTCCCGGCCTGTAA971271.174093473443973No Hit
TATTGCACTTGTCCCGGCCTGTAT922231.1148127956327645No Hit
TATTGCACTTGTCCCGGCCTGT795870.9620659267864288No Hit
TGAGGTAGTAGGTTGTATAGTT746710.9026401902203804No Hit
TAGCTTATCAGACTGATGTTGACT721200.8718031165873478No Hit
AACCGGGCGGAAACACCA651040.7869920979243302No Hit
TGTAAACATCCTTGACTGGAAGCT599230.7243629805222357No Hit
AGCAGCATTGTACAGGGCTATGA551200.6663032138975957No Hit
TATTGCACTTGTCCCGGCCTGTA534090.6456202531033507No Hit
TAGCTTATCAGACTGATGTTGA517350.6253845568031952No Hit
CGCCTGTCTGAGCGTCGCT476470.5759678743210949No Hit
TTCAAGTAATTCAGGATAGGTT445290.5382767745218803No Hit
TGAGGTAGTAGATTGTATAGTT443640.5362822166428327No Hit
CTCCCACTGCTTCACTTGACTAG435260.526152280263185No Hit
TCGTACGACTCTTAGCGG430260.5201081654781922No Hit
ACTGGACTTGGAGTCAGAAGGC411140.49699547054038007No Hit
TGTAAACATCCCCGACTGGAAGCT391820.47364101101116834No Hit
TTCTCACTACTGCACTTGACTAG371840.4494887283303375No Hit
CAACGGAATCCCAAAAGCAGCTGT355860.4301717374775008No Hit
CATAAAGTAGAAAGCACTACT315610.3815166134583095No Hit
CCCCCACTGCTAAATTTGACTGGCTT305350.36911408991950445No Hit
CTTCTCACTACTGCACTTGACTAG292930.3541005087935826No Hit
CCCCCCACTGCTAAATTTGACTGG283190.34232657319241677No Hit
CCCCCCACTGCTAAATTTGACTGGCT279000.33726160500259295No Hit
TCCTGTCTGAGCGTCGCT269850.32620087494605626No Hit
CATTGCACTTGTCTCGGTCTGA267610.32349311152237953No Hit
CCCATAAAGTAGAAAGCACTAC255300.3086125009217275No Hit
TGTAAACATCCTACACTCTCAGCT253050.3058926492684808No Hit
CACCCGGCTGTGTGCACATGTGC247010.29859135860820957No Hit
AACATTCAACGCTGTCGGTGAGTT234290.28321513059518816No Hit
CTACGCCTGTCTGAGCGTCGCT213360.2579144661052087No Hit
CTCCCACTGCTTCACTTGACTAGCCT190830.23067968488403157No Hit
GCGTTCGCGCTTTCCCCTG183990.22241133585816156No Hit
TACGCCTGTCTGAGCGTCGCT183950.2223629829398816No Hit
TGAGGTAGTAGTTTGTACAGTT169680.20511307934351244No Hit
ACCCCCCACTGCTAAATTTGACTG167820.20286466864349512No Hit
CGCGACCTCAGATCAGACGTGG165370.19990305239884873No Hit
TGTCAGTTTGTCAAATACCCCAA158780.19193690911222833No Hit
CCCATAAAGTAGAAAGCACT158160.19118743887888923No Hit
CAACGGAATCCCAAAAGCAGCT154480.1867389703971346No Hit
AGCTCGGTCTGAGGCCCCTCAGT153850.18597741193422554No Hit
TTAATGCTAATCGTGATAGGGGTT150900.18241138421107983No Hit
TTCAAGTAATCCAGGATAGGCTT148750.17981241485353297No Hit
CGCGACCTCAGATCAGACGTGGCG148010.17891788586535404No Hit
ACGCCTGTCTGAGCGTCGCT141160.17063744860991403No Hit
TGTCAGTTTGTCAAATACCCCA137570.16629777419428926No Hit
CCCCCCACTGCTAAATTTGACTGTA136930.1655241275018102No Hit
TATTGCACTTGTCCCGGCCTGTT133650.161559188202855No Hit
AACTTAACTTGACCGCTCTGACCA132590.16027783586843655No Hit
TGAGGTAGTAGTTTGTGCTGTT132110.15969760084907725No Hit
AACGGAATCCCAAAAGCAGCTG130160.15734039608293007No Hit
TAGCTTATCAGACTGATGTTGACA127030.15355678022752464No Hit
CCCCCCACTGCTAAATTTGACTGT126860.15335128032483492No Hit
GGGCTACGCCTGTCTGAGCGTCGCT125500.15170728110331688No Hit
CACTAGATTGTGAGCTCCTGGA124070.14997866427480896No Hit
TCCCACTGCTTCACTTGACTAG121650.1470533127188725No Hit
GGAATACCGGGTGCTGTAGGCTTT119590.14456313742745552No Hit
TATTGCACTTGTCCCGGCCTGTAG118390.14311254987905725No Hit
TAGCAGCACGTAAATATTGGC116650.1410091979338798No Hit
CCCCCACTGCTAAATTTGACTGGCT116240.14051358052151042No Hit
TCAGTGCACTACAGAACTTTGT113450.13714096447148447No Hit
TCGTTTCCCGGGCGGCGCACCA113270.13692337633922472No Hit
CAAAGTGCTGTTCGTGCAGGTAG112330.13578708275964613No Hit
TGAGGTAGTAGGTTGTGTGGTT109400.13224523149564038No Hit
TTCAAGTAATCCAGGATAGGC109180.1319792904451007No Hit
ACCACAGGGTAGAACCACGGAC107280.12968252682680348No Hit
CACTAGATTGTGAGCTCCTGGAA106880.12919899764400405No Hit
AGCAGCATTGTACAGGGCTATGT106000.12813523344184533No Hit
TGTAAACATCCCCGACTGGAAGC105570.12761543957033597No Hit
TGATGTAGTAGGTTGTATAGTT102700.12414611768375015No Hit
TGTAAACATCCTACACTCAGCT101130.12224826564126245No Hit
CTCCCACTGCTTCACTTGACTA99040.11972182566113548No Hit
CAAAGAATTCTCCTTTTGGGCT98770.11939544346274589No Hit
ATCCCACCGCTGCCACCA98440.11899653188693637No Hit
GGAATACCGGGTGCTGTAGGCTT97480.11783606184821777No Hit
TAGCAGCACGTAAATATTGGCGT97420.11776353247079786No Hit
GACCCGCGGGCGCTCTCCAGTCCT94620.11437882819120196No Hit
TTCACCACCTTCTCCACCCAGC93560.1130974758567835No Hit
CAACACCAGTCGATGGGCTGT91310.11037762420353678No Hit
CCTCGACACAAGGGTTTGT90300.10915671301696825No Hit
TTATAAAGCAATGAGACTGATT86600.10468406807607365No Hit
CAAAGAATTCTCCTTTTGGGCTT85580.10345106865993514No Hit
TCAGTGCACTACAGAACTTTGTC84250.10184333412712708No Hit
TCGTTTCCCGGCCAATGCACCA83670.10114221681206792No Hit
CAAAGTGCTGTTCGTGCAGGT83050.10039274657872882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)