FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7605278
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT100889113.2656689209783No Hit
CCCCCCACTGCTAAATTTGACTG6908549.083875697903482No Hit
CAACGGAATCCCAAAAGCAGCTG6621078.705888200273547No Hit
CCCCCACTGCTAAATTTGACTG3273594.304365994247679No Hit
TTCAAGTAATCCAGGATAGGCT2541103.341232233719793No Hit
TAGCAGCACGTAAATATTGGCG2345683.0842791019605067No Hit
AAGCTGCCAGTTGAAGAACTGT1752372.304149828579573No Hit
AACATTCAACGCTGTCGGTGAGT1444101.8988129033547492No Hit
AACATTCAACGCTGTCGGTGAG1255341.6506168479311343No Hit
TGAGGTAGTAGGTTGTATAGTT1047151.3768727454801784No Hit
CATAAAGTAGAAAGCACTACT903701.1882537364183137No Hit
TAGCTTATCAGACTGATGTTGAC901151.1849008017852865No Hit
TCGTACGACTCTTAGCGG853731.122549366374247No Hit
TAGCTTATCAGACTGATGTTGACT733520.9644880831443637No Hit
TAGCTTATCAGACTGATGTTGA725500.9539427750044114No Hit
TATTGCACTTGTCCCGGCCTGTAA660060.8678972681866461No Hit
TATTGCACTTGTCCCGGCCTGT614350.8077942712942248No Hit
TATTGCACTTGTCCCGGCCTGTAT595850.7834690592506941No Hit
TGAGGTAGTAGATTGTATAGTT562640.7398020164417396No Hit
TGTAAACATCCTTGACTGGAAGCT493550.648957211031602No Hit
TCCTGTCTGAGCGTCGCT441070.579952501407575No Hit
TTCAAGTAATTCAGGATAGGTT422560.5556141406007775No Hit
CCCATAAAGTAGAAAGCACTAC368420.48442673627446625No Hit
TTCTCACTACTGCACTTGACTAG360770.47436793237538455No Hit
TATTGCACTTGTCCCGGCCTGTA359740.4730136097589069No Hit
CAACGGAATCCCAAAAGCAGCTGT342150.4498849351726525No Hit
CACCCGGCTGTGTGCACATGTGC319090.41956388707947295No Hit
CATTGCACTTGTCTCGGTCTGA311640.4097680584457268No Hit
AGCAGCATTGTACAGGGCTATGA294790.3876123923412136No Hit
CCCATAAAGTAGAAAGCACT280010.36817852023292247No Hit
AACCGGGCGGAAACACCA276210.36318199019154856No Hit
CCCCCACTGCTAAATTTGACTGGCTT244590.321605600742011No Hit
TGTAAACATCCCCGACTGGAAGCT237180.3118623671613319No Hit
CTCCCACTGCTTCACTTGACTAG236690.31121807776126004No Hit
ACTGGACTTGGAGTCAGAAGGC229030.3011461250989116No Hit
TCAGTGCACTACAGAACTTTGT221320.29100842862022924No Hit
TGAGGTAGTAGTTTGTACAGTT210360.27659738407984563No Hit
TCAGTGCACTACAGAACTTTGTC208590.2742700529816267No Hit
CTTCTCACTACTGCACTTGACTAG205910.27074618442613146No Hit
CAACGGAATCCCAAAAGCAGCT188920.2484064356358834No Hit
AACGGAATCCCAAAAGCAGCTG181160.23820299534086722No Hit
CAACACCAGTCGATGGGCTGT173730.22843346423365457No Hit
TGAGAACTGAATTCCATGGGTT171250.2251725709434948No Hit
TAGCAGCACGTAAATATTGGC166080.21837466033457292No Hit
TGTAAACATCCTACACTCTCAGCT155880.20496292180246403No Hit
CCCCCCACTGCTAAATTTGACTGG154390.2030037560757148No Hit
CGCCTGTCTGAGCGTCGCT142180.18694911612698445No Hit
CGCGACCTCAGATCAGACGTGG141630.18622593414731192No Hit
ACCCCCCACTGCTAAATTTGACTG134360.17666678325236762No Hit
TGAGGTAGTAGTTTGTGCTGTT130480.17156506310485956No Hit
TTCACAGTGGCTAAGTTCCGC125550.16508272281434025No Hit
TTCAAGTAATCCAGGATAGGC121410.15963913482189607No Hit
CCCCCCACTGCTAAATTTGACTGGCT110630.1454647680203143No Hit
TTCAAGTAATCCAGGATAGGCTT104170.13697066694997867No Hit
TTCACAGTGGCTAAGTTCTGC103190.13568208814983487No Hit
TATTGCACTTGTCCCGGCCTGTT103170.13565579062330135No Hit
CACTAGATTGTGAGCTCCTGGA99720.1311194672962645No Hit
CCCATAAAGTAGAAAGCACTA97420.12809525174490663No Hit
TGTCAGTTTGTCAAATACCCCA97190.12779283018977083No Hit
AGCTCGGTCTGAGGCCCCTCAGT96310.12663573902229477No Hit
TGTCAGTTTGTCAAATACCCCAA95970.12618868107122447No Hit
TTATAAAGCAATGAGACTGATT95870.12605719343855676No Hit
CAAAGAATTCTCCTTTTGGGCT95400.1254392015650184No Hit
TCCCACTGCTTCACTTGACTAG94230.12390079626280592No Hit
CTCCCACTGCTTCACTTGACTAGCCT94080.1237035648138043No Hit
TTAATGCTAATCGTGATAGGGGTT92580.12173125032378829No Hit
TAGCTTATCAGACTGATGTTGACA89780.11804959660909176No Hit
TGATGTAGTAGGTTGTATAGTT89310.11743160473555339No Hit
AACATTCAACGCTGTCGGTGAGTT88140.1158931994333409No Hit
TGAGGTAGTAGGTTGTATAGT86310.11348697575552136No Hit
TGAGGTAGTAGGTTGTGTGGTT84910.11164614889817308No Hit
GCCTGTCTGAGCGTCGCG84270.11080462804909957No Hit
TCGTTTCCCGGCCAATGCACCA83210.1094108591428216No Hit
CTACGCCTGTCTGAGCGTCGCT80700.10611051956286148No Hit
TGAGAACTGAATTCCATAGGCTGT80490.10583439553425923No Hit
TGTAAACATCCCCGACTGGAAGC80450.10578180048119214No Hit
ATCACATTGCCAGGGATTTCC79810.10494027963211863No Hit
TGTAAACATCCTACACTCAGCT78270.10291537008903553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)