FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10110002
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG126570212.519305139603334No Hit
CAACGGAATCCCAAAAGCAGCTG8955778.858326635345868No Hit
CCCCCACTGCTAAATTTGACTG4404674.3567449343729105No Hit
GCCTGTCTGAGCGTCGCT4150514.105350325351073No Hit
TAGCAGCACGTAAATATTGGCG3193553.1588025402962336No Hit
TTCAAGTAATCCAGGATAGGCT2951282.919168561984459No Hit
AACATTCAACGCTGTCGGTGAGT2615082.586626590182673No Hit
AAGCTGCCAGTTGAAGAACTGT2235022.210701837645532No Hit
AACATTCAACGCTGTCGGTGAG1933251.9122152498090506No Hit
TGAGGTAGTAGGTTGTATAGTT989430.9786644948240367No Hit
TAGCTTATCAGACTGATGTTGAC973880.9632836867885882No Hit
TATTGCACTTGTCCCGGCCTGTAA958540.9481105938455799No Hit
TCCTGTCTGAGCGTCGCT929630.9195151494529872No Hit
TATTGCACTTGTCCCGGCCTGTAT858100.8487634324899244No Hit
TATTGCACTTGTCCCGGCCTGT786420.7778633476036899No Hit
TAGCTTATCAGACTGATGTTGACT737410.7293866015061125No Hit
GGAATACCGGGTGCTGTAGGCTTT710300.7025715721915783No Hit
TTCAAGTAATTCAGGATAGGTT616530.6098218378196167No Hit
TAGCTTATCAGACTGATGTTGA590870.5844410317624071No Hit
TGTAAACATCCTTGACTGGAAGCT581440.575113634992357No Hit
TTCTCACTACTGCACTTGACTAG571110.5648960306832778No Hit
CATAAAGTAGAAAGCACTACT548290.5423243239714493No Hit
GGAATACCGGGTGCTGTAGGCTT545490.5395547894055808No Hit
TGAGGTAGTAGATTGTATAGTT534560.5287437134038153No Hit
TATTGCACTTGTCCCGGCCTGTA507750.5022254199356242No Hit
CAACGGAATCCCAAAAGCAGCTGT501960.49649841810120315No Hit
CCCCCACTGCTAAATTTGACTGGCTT501790.49633026778827544No Hit
CAACGGAATCCCAAAAGCAGCT498860.4934321476889916No Hit
AACCGGGCGGAAACACCA494520.4891393691118954No Hit
AGCAGCATTGTACAGGGCTATGA461160.4561423429985474No Hit
CTCCCACTGCTTCACTTGACTAG424620.41999991691396305No Hit
TGTAAACATCCCCGACTGGAAGCT372690.3686349419119799No Hit
CACCCGGCTGTGTGCACATGTGC348360.3445696647735579No Hit
CCCATAAAGTAGAAAGCACTAC346110.342344145925985No Hit
CTTCTCACTACTGCACTTGACTAG341810.3380909321284012No Hit
ACTGGACTTGGAGTCAGAAGGC326710.32315522786246725No Hit
CATTGCACTTGTCTCGGTCTGA320360.31687431911487257No Hit
CCCCCCACTGCTAAATTTGACTGGCT318000.3145399971236405No Hit
CCCCCCACTGCTAAATTTGACTGG313180.30977244119239544No Hit
TCGTACGACTCTTAGCGG277030.274015771708057No Hit
TTAATGCTAATCGTGATAGGGGTT273210.27023733526462207No Hit
CCCATAAAGTAGAAAGCACT257180.25438174987502477No Hit
ACCCCCCACTGCTAAATTTGACTG245230.24256177199569298No Hit
TGAGGTAGTAGTTTGTACAGTT242700.2400592996915332No Hit
CGCCTGTCTGAGCGTCGCT241570.23894159467030768No Hit
CTACGCCTGTCTGAGCGTCGCT230360.22785356521195543No Hit
AACGGAATCCCAAAAGCAGCTG221910.21949550553995936No Hit
CTCCCACTGCTTCACTTGACTAGCCT218170.21579619865554922No Hit
TAGCAGCACGTAAATATTGGC209200.20692379684989182No Hit
TGTAAACATCCTACACTCTCAGCT203070.20086049438961537No Hit
CGCGACCTCAGATCAGACGTGG201650.1994559447169249No Hit
TGAGAACTGAATTCCATGGGTT193810.191701247932493No Hit
AGCTCGGTCTGAGGCCCCTCAGT171740.1698713808365221No Hit
AACATTCAACGCTGTCGGTGAGTT165110.16331351863234053No Hit
TGAATACCGGGTGCTGTAGGCTTT164630.16283874127819162No Hit
CCCCCACTGCTAAATTTGACTGGCT152920.15125615207593432No Hit
GAATACCGGGTGCTGTAGGCTTT152240.15058355082422337No Hit
CAACACCAGTCGATGGGCTGT151190.1495449753620227No Hit
TGAGGTAGTAGTTTGTGCTGTT150470.14883280933079934No Hit
CAAAGAATTCTCCTTTTGGGCT149690.14806129613030738No Hit
CACTAGATTGTGAGCTCCTGGA145620.14403557981491993No Hit
TGTCAGTTTGTCAAATACCCCAA143390.1418298433571032No Hit
CAACGGAATCCCAAAAGCAGCTGA143350.14179027857759077No Hit
GGGAATACCGGGTGCTGTAGGCTTT143320.14176060499295648No Hit
TCAGTGCACTACAGAACTTTGT142310.1407615943102682No Hit
TGATGTAGTAGGTTGTATAGTT139800.1382789043958646No Hit
TTCAAGTAATCCAGGATAGGCTT137120.1356280641685333No Hit
TCAGTGCACTACAGAACTTTGTC136820.13533132832219025No Hit
CCCCCCACTGCTAAATTTGACTGTA136660.13517306920414063No Hit
TCGTTTCCCGGCCAATGCACCA135820.13434220883438006No Hit
TTCAAGTAATCCAGGATAGGC134990.13352123965949758No Hit
TATTGCACTTGTCCCGGCCTGTT134150.13269037928973704No Hit
TCCCACTGCTTCACTTGACTAG132720.13127593842216845No Hit
CCCCCCACTGCTAAATTTGACTGT132600.13115724408363122No Hit
TGTCAGTTTGTCAAATACCCCA132060.13062311956021372No Hit
TGAGGTAGTAGGTTGTGTGGTT127990.12659740324482627No Hit
GACCCGCGGGCGCTCTCCAGTCCT127550.12616219067018977No Hit
TGAATACCGGGTGCTGTAGGCTT124260.12290798755529425No Hit
ATCCCGGACGAGCCCCCA123480.12213647435480231No Hit
CACTAGATTGTGAGCTCCTGGAA122080.12075170707186804No Hit
GGGAATACCGGGTGCTGTAGGCTT121020.11970324041478925No Hit
TAGCTTATCAGACTGATGTTGACA120740.11942628695820237No Hit
TGTAAACATCCCCGACTGGAAGC120490.11917900708624983No Hit
ACCGGGTGCTGTAGGCTT120240.11893172721429729No Hit
GAATACCGGGTGCTGTAGGCTT120060.11875368570649146No Hit
GGAATACCGGGTGCTGTAGGCTTA119870.11856575300380752No Hit
ACCGGGTGCTGTAGGCTTT119010.11771511024429074No Hit
TATTGCACTTGTCCCGGCCTGTAG115540.11428286562158939No Hit
TACGCCTGTCTGAGCGTCGCT115280.11402569455475874No Hit
CGCGACCTCAGATCAGACGTGGCG114400.11315526940548577No Hit
CCCCCACTGCTAAATTTGACTGG112910.11168148136864858No Hit
TTCACAGTGGCTAAGTTCTGC112240.11101877131181577No Hit
CCCTGTCTGAGCGTCGCT106990.10582589400081226No Hit
AATGCACCCGGGCAAGGATTCT106910.10574676444178743No Hit
ACCGGGTGCTGTAGGCTTA105810.10465873300519624No Hit
AATGACACGATCACTCCCGTTG104270.10313548899396854No Hit
CTCCCACTGCTTCACTTGACTA101120.10001976260736645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)