FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6119419
Sequences flagged as poor quality0
Sequence length18-26
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT200730632.80223171513505No Hit
CAACGGAATCCCAAAAGCAGCTG2455164.012080231799783No Hit
CCCCCCACTGCTAAATTTGACTG2156323.5237332171567273No Hit
TTCAAGTAATCCAGGATAGGCT1489412.4339075327249207No Hit
CCCCCACTGCTAAATTTGACTG1254942.0507502427926574No Hit
TAGCAGCACGTAAATATTGGCG1244612.0338695552633346No Hit
CATAAAGTAGAAAGCACTACT943011.5410123085214462No Hit
TGAGGTAGTAGGTTGTATAGTT840221.3730388456812648No Hit
AAGCTGCCAGTTGAAGAACTGT792581.2951883177144758No Hit
AACATTCAACGCTGTCGGTGAGT758561.239594804670182No Hit
TCGTACGACTCTTAGCGG649701.0617020994967006No Hit
TAGCTTATCAGACTGATGTTGA620941.0147041737132234No Hit
AACATTCAACGCTGTCGGTGAG570860.9328663391083369No Hit
TGAGGTAGTAGATTGTATAGTT567730.9277514744455315No Hit
TAGCTTATCAGACTGATGTTGAC506620.8278890528659665No Hit
AACCGGGCGGAAACACCA505920.8267451534206107No Hit
TAGCTTATCAGACTGATGTTGACT375790.6140942465289597No Hit
TTCAAGTAATTCAGGATAGGTT355280.5805779927800335No Hit
TATTGCACTTGTCCCGGCCTGT270180.441512503066059No Hit
CGCCTGTCTGAGCGTCGCT237140.3875204492452633No Hit
TTCTCACTACTGCACTTGACTAG234340.38294485146383994No Hit
TGTAAACATCCTTGACTGGAAGCT227260.371375125645098No Hit
TATTGCACTTGTCCCGGCCTGTAA213160.34833372253150174No Hit
TCCTGTCTGAGCGTCGCT200900.3282991408171266No Hit
CATTGCACTTGTCTCGGTCTGA200650.32789060530092806No Hit
TGAGGTAGTAGTTTGTACAGTT197010.3219423281850777No Hit
TATTGCACTTGTCCCGGCCTGTAT188900.30868943603959786No Hit
CAACACCAGTCGATGGGCTGT169420.2768563486174096No Hit
CCCATAAAGTAGAAAGCACTAC155910.25477908932204185No Hit
TCAGTGCACTACAGAACTTTGT151260.24718032872074946No Hit
GCCGGTCTGAGCGTCGCT150530.2459874050134498No Hit
AGCAGCATTGTACAGGGCTATGA141360.23100232227928827No Hit
AGAATTACTCTGAGACCT139220.227505258260629No Hit
GCCTGTCTTAGCGTCGCT138770.22676989433147166No Hit
TATTGCACTTGTCCCGGCCTGTA133070.21745528456214555No Hit
CAACGGAATCCCAAAAGCAGCTGT130000.21243846842322775No Hit
GCCTGTCTGAGCGTCGCG123350.20157142369234726No Hit
TGATGTAGTAGGTTGTATAGTT120060.1961950962991748No Hit
CTTCTCACTACTGCACTTGACTAG111340.1819453774941706No Hit
TCAGTGCACTACAGAACTTTGTC108250.17689587851395697No Hit
ACTGGACTTGGAGTCAGAAGGC108170.17676514714877342No Hit
CACCCGGCTGTGTGCACATGTGC106530.1740851541625112No Hit
GCCTGTCTGAGCGTCGCTT105010.17160125822402422No Hit
CTCCCACTGCTTCACTTGACTAG104040.17001614042117397No Hit
TGAGGTAGTAGTTTGTGCTGTT103390.16895394807905784No Hit
TAGTGGGTTATCAGAACT101250.16545688406039855No Hit
TTATAAAGCAATGAGACTGATT100640.1644600574008742No Hit
TCCTTCTGATCGAGGCCC99980.1633815236381101No Hit
TTCACAGTGGCTAAGTTCCGC99560.16269518397089658No Hit
TGTAAACATCCCCGACTGGAAGCT99500.16259713544700896No Hit
CCCATAAAGTAGAAAGCACT88700.1449484011472331No Hit
TTCACAGTGGCTAAGTTCCG85850.14029109626257003No Hit
TAGCAGCACGTAAATATTGGC82720.1351762315997646No Hit
TGAGGTAGTAGGTTGTATAGT81910.1338525765272814No Hit
CCCCCACTGCTAAATTTGACTGGCTT81810.13368916232080202No Hit
TTCAAGTAATCCAGGATAGGC81100.13252892145479825No Hit
TGATGTAGTAGATTGTATAGTT80940.13226745872443121No Hit
GCCTGTCTGAGCGTCGCA79230.12947307579363335No Hit
CTACGCCTGTCTGAGCGTCGCT76910.12568186620331112No Hit
CAACGGAATCCCAAAAGCAGCT76790.12548576915553583No Hit
NCCTGTCTGAGCGTCGCT75280.12301821463769681No Hit
TGAGGTAGTAAGTTGTATTGTT73550.1201911488656031No Hit
TTCACAGTGGCTAAGTTCTGC71090.1161711593862097No Hit
AACGGAATCCCAAAAGCAGCTG70180.1146840901072471No Hit
TGTAAACATCCTACACTCTCAGCT68590.11208580422422455No Hit
TTCACAGTGGCTAAGTTCTG68480.11190604859709721No Hit
GCCTGTATGAGCGTCGCT67060.10958556686508963No Hit
TCGACCGGACCTCGACCGGCT66000.10785337627640794No Hit
TGTCAGTTTGTCAAATACCCCA65840.1075919135460409No Hit
TGAGGTAGTAGATTGTATAGT64030.10463411640876363No Hit
GCCTGTCTGAGCGTAGCT63830.10430728799580484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)