FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8019808
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7164108.933006874977556No Hit
CAACGGAATCCCAAAAGCAGCTG4443985.541254852984012No Hit
AACATTCAACGCTGTCGGTGAGT2493383.10902704902661No Hit
TTCAAGTAATCCAGGATAGGCT2187932.7281575818273955No Hit
AAGCTGCCAGTTGAAGAACTGT2147132.6772835459402518No Hit
CCCCCACTGCTAAATTTGACTG2130952.6571084993555956No Hit
TAGCAGCACGTAAATATTGGCG2057922.5660464689428975No Hit
TAGCTTATCAGACTGATGTTGAC1403381.7498922667475332No Hit
AACATTCAACGCTGTCGGTGAG1395841.7404905454095658No Hit
TATTGCACTTGTCCCGGCCTGTAA1087371.3558554020245872No Hit
TATTGCACTTGTCCCGGCCTGTAT1052301.312126175589241No Hit
TAGCTTATCAGACTGATGTTGACT1039291.2959038420869926No Hit
TGTAAACATCCTTGACTGGAAGCT823061.0262839210115753No Hit
TATTGCACTTGTCCCGGCCTGT764120.9527908897569617No Hit
TGAGGTAGTAGGTTGTATAGTT741550.9246480713752747No Hit
TAGCTTATCAGACTGATGTTGA662140.8256307382919891No Hit
AGCAGCATTGTACAGGGCTATGA615520.7674996708150619No Hit
TATTGCACTTGTCCCGGCCTGTA518520.646549144318667No Hit
GCCTGTCTGAGCGTCGCT510930.6370850773484852No Hit
TGAGGTAGTAGATTGTATAGTT446800.5571205694699922No Hit
TGTAAACATCCCCGACTGGAAGCT429850.5359854001492306No Hit
TTCAAGTAATTCAGGATAGGTT416280.5190647955661781No Hit
TTCTCACTACTGCACTTGACTAG409360.5104361600676725No Hit
ACTGGACTTGGAGTCAGAAGGC405710.5058849289160039No Hit
CTCCCACTGCTTCACTTGACTAG383150.47775457966075996No Hit
TTAATGCTAATCGTGATAGGGGTT336620.41973573432181915No Hit
CATAAAGTAGAAAGCACTACT320210.3992738978289755No Hit
CGCCTGTCTGAGCGTCGCT316220.3942987163782475No Hit
CAACGGAATCCCAAAAGCAGCTGT295140.3680137978365567No Hit
CCCCCACTGCTAAATTTGACTGGCTT293100.36547009604219954No Hit
CTTCTCACTACTGCACTTGACTAG292690.3649588618580395No Hit
CCCATAAAGTAGAAAGCACTAC270290.33702801862588233No Hit
TGTAAACATCCTACACTCTCAGCT250480.3123266791424433No Hit
CGCGACCTCAGATCAGACGTGGCG245190.30573051125413475No Hit
CCCCCCACTGCTAAATTTGACTGG244250.3045584133684996No Hit
TCGTACGACTCTTAGCGG237040.295568173203149No Hit
CCCCCCACTGCTAAATTTGACTGGCT236820.2952938524214046No Hit
CATTGCACTTGTCTCGGTCTGA230700.2876627470383331No Hit
AACATTCAACGCTGTCGGTGAGTT226450.28236336830008896No Hit
TAGCTTATCAGACTGATGTTGACA201740.2515521568596156No Hit
CTACGCCTGTCTGAGCGTCGCT186970.23313525710341193No Hit
ACCCCCCACTGCTAAATTTGACTG186060.23200056659710555No Hit
AGCTCGGTCTGAGGCCCCTCAGT169800.21172576700090578No Hit
GGAATACCGGGTGCTGTAGGCTTT167530.20889527529836127No Hit
CTCCCACTGCTTCACTTGACTAGCCT166030.20702490633192216No Hit
TGAGGTAGTAGTTTGTACAGTT163010.2032592301461581No Hit
TACGCCTGTCTGAGCGTCGCT160810.20051602232871413No Hit
CCCCCCACTGCTAAATTTGACTGTA156110.19465553290053825No Hit
CAAAGAATTCTCCTTTTGGGCT150070.18712418052901017No Hit
TTCAAGTAATCCAGGATAGGCTT148270.18487973776928326No Hit
GTGCGGTAACGCGACCGATCCCGGA143930.1794681368930528No Hit
TATTGCACTTGTCCCGGCCTGTT142370.17752295316795613No Hit
GGAATACCGGGTGCTGTAGGCTT137950.17201159928018225No Hit
CAACGGAATCCCAAAAGCAGCT135860.16940555185361045No Hit
CCCATAAAGTAGAAAGCACT135000.1683332069795187No Hit
CGCGACCTCAGATCAGACGTGG133830.16687431918569623No Hit
AACCGGGCGGAAACACCA133460.16641296150730792No Hit
ACCGGGTGCTGTAGGCTT132850.16565234479428934No Hit
TGAGGTAGTAGTTTGTGCTGTT131910.16448024690865418No Hit
AGCAGCATTGTACAGGGCTATGT130500.1627221000802014No Hit
TATTGCACTTGTCCCGGCCTGTAG125440.1564127221000802No Hit
ACCGGGTGCTGTAGGCTTA122410.15263457678787323No Hit
TAGCAGCACGTAAATATTGGC121410.1513876641435805No Hit
TGTAAACATCCCCGACTGGAAGC120230.14991630722331506No Hit
CCCCCCACTGCTAAATTTGACTGT113820.14192359717339867No Hit
CAAAGAATTCTCCTTTTGGGCTT113390.1413874247363528No Hit
AACGGAATCCCAAAAGCAGCTG109780.13688607009045603No Hit
TCCCACTGCTTCACTTGACTAG109730.1368237244582414No Hit
CACTAGATTGTGAGCTCCTGGA107330.13383113411193884No Hit
CACTAGATTGTGAGCTCCTGGAA105530.13158669135221193No Hit
CAAAGTGCTGTTCGTGCAGGTAG103780.12940459422469966No Hit
TGTCAGTTTGTCAAATACCCCA102740.1281078050746352No Hit
TCCTGTCTGAGCGTCGCT102270.1275217561318176No Hit
ACCGGGTGCTGTAGGCTTT99970.12465385704994435No Hit
TTCAAGTAATCCAGGATAGGC98040.12224731564645937No Hit
GGGCTACGCCTGTCTGAGCGTCGCT97860.12202287137048667No Hit
TGTCAGTTTGTCAAATACCCCAA97830.12198546399115788No Hit
TTATAAAGCAATGAGACTGATT97280.12129966203679689No Hit
TGTAAACATCCTACACTCAGCT95550.11914250316217047No Hit
ACGCCTGTCTGAGCGTCGCT95280.11880583674821142No Hit
TATTGCACTTGTCCCGGCCTGTTT94910.11834447906982311No Hit
TGAGGTAGTAGGTTGTGTGGTT94800.11820731867895093No Hit
GCGTTCGCGCTTTCCCCTG94630.11799534352942116No Hit
ACCACAGGGTAGAACCACGGAC93050.11602522155143863No Hit
TAGCAGCACGTAAATATTGGCGT92050.11477830890714591No Hit
TGATGTAGTAGGTTGTATAGTT90330.1126336191589624No Hit
TTCACAGTGGCTAAGTTCCG90250.11253386614741899No Hit
CAACACCAGTCGATGGGCTGT88200.10997769522661889No Hit
CCCCCACTGCTAAATTTGACTGGCT86960.1084315235476959No Hit
AGCAGCATTGTACAGGGCTATG85200.1062369572937407No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT84860.10581300699468116No Hit
TATTGCACTTGTCCCGGCCTGTAAT83820.10451621784461672No Hit
ATCACATTGCCAGGGATTTCCA81440.10154856575120004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)