FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7388342
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6086798.238370665570164No Hit
CCCCCCACTGCTAAATTTGACTG5200887.039305976902531No Hit
GCCTGTCTGAGCGTCGCT4431035.997326599120615No Hit
CCCCCACTGCTAAATTTGACTG2227743.0152096370200514No Hit
TTCAAGTAATCCAGGATAGGCT2179152.9494438671084797No Hit
TAGCAGCACGTAAATATTGGCG2177392.9470617359077314No Hit
AAGCTGCCAGTTGAAGAACTGT1932162.6151469436579955No Hit
AACATTCAACGCTGTCGGTGAGT1864992.5242334477748862No Hit
AACATTCAACGCTGTCGGTGAG1378911.8663321216045494No Hit
TGAGGTAGTAGGTTGTATAGTT895231.2116791561625058No Hit
TCGTACGACTCTTAGCGG772781.0459450848377079No Hit
TATTGCACTTGTCCCGGCCTGT741961.0042307191518747No Hit
TATTGCACTTGTCCCGGCCTGTAA650060.8798455729309769No Hit
CATAAAGTAGAAAGCACTACT631660.8549414740140615No Hit
TATTGCACTTGTCCCGGCCTGTAT588500.7965251202502537No Hit
TAGCTTATCAGACTGATGTTGAC570530.7722030192971576No Hit
TAGCTTATCAGACTGATGTTGA540440.7314766966661803No Hit
TAGCTTATCAGACTGATGTTGACT499990.6767282835580704No Hit
TGAGGTAGTAGATTGTATAGTT375050.5076240379776681No Hit
ACCGGGTGCTGTAGGCTT373040.5049035358677224No Hit
TATTGCACTTGTCCCGGCCTGTA371150.5023454517941914No Hit
TGTAAACATCCTTGACTGGAAGCT365550.49476594342817376No Hit
CAACGGAATCCCAAAAGCAGCTGT364730.4936560868460068No Hit
TTCTCACTACTGCACTTGACTAG336890.45597510239780453No Hit
TTCAAGTAATTCAGGATAGGTT301720.40837308289193974No Hit
CCCATAAAGTAGAAAGCACTAC276430.3741434817175491No Hit
AGCAGCATTGTACAGGGCTATGA276340.3740216681902381No Hit
CGCCTGTCTGAGCGTCGCT273310.36992061277076776No Hit
ACTGGACTTGGAGTCAGAAGGC258840.35033570454643276No Hit
CTCCCACTGCTTCACTTGACTAG245570.3323749766862444No Hit
TTAATGCTAATCGTGATAGGGGTT241330.32663620606625954No Hit
CATTGCACTTGTCTCGGTCTGA236000.3194221382821748No Hit
ACCGGGTGCTGTAGGCTTT235500.3187453964637804No Hit
CCCCCACTGCTAAATTTGACTGGCTT230350.3117749557343177No Hit
TGTAAACATCCCCGACTGGAAGCT219170.296643008675018No Hit
TCCTGTCTGAGCGTCGCT202100.2735390429950319No Hit
CCCATAAAGTAGAAAGCACT199240.26966807979381574No Hit
ACCGGGTGCTGTAGGCTTA195920.26517451411967663No Hit
CAACGGAATCCCAAAAGCAGCT188360.25494217782555273No Hit
CAACACCAGTCGATGGGCTGT183010.24770104036873228No Hit
CTTCTCACTACTGCACTTGACTAG177780.24062232094832645No Hit
TGAGAACTGAATTCCATGGGTT176970.23952599920252743No Hit
TGAGGTAGTAGTTTGTACAGTT166830.22580167512548824No Hit
AACGGAATCCCAAAAGCAGCTG165510.2240150767249269No Hit
CGCGACCTCAGATCAGACGTGG163160.22083439017847303No Hit
CCCCCCACTGCTAAATTTGACTGG160490.21722058886824674No Hit
TAGCAGCACGTAAATATTGGC149990.20300901068196356No Hit
ATCCCGGACGAGCCCCCA148760.20134422580871322No Hit
CTACGCCTGTCTGAGCGTCGCT148620.2011547380995628No Hit
AGCTCGGTCTGAGGCCCCTCAGT138540.1875116230407309No Hit
CCCCCCACTGCTAAATTTGACTGGCT137810.18652357998587504No Hit
ACCCCCCACTGCTAAATTTGACTG124890.16903657139856276No Hit
TGAGGTAGTAGTTTGTGCTGTT124240.16815680703465No Hit
TATTGCACTTGTCCCGGCCTGTT119260.1614164585234414No Hit
AACATTCAACGCTGTCGGTGAGTT116570.1577755875404793No Hit
TGAGGTAGTAGGTTGTGTGGTT115010.15566415306708867No Hit
CGCGACCTCAGATCAGACGTGGCG112710.15255114070247425No Hit
TGTAAACATCCTACACTCTCAGCT111840.15137360993846793No Hit
TTCACAGTGGCTAAGTTCCGC109710.14849068979210764No Hit
CTCCCACTGCTTCACTTGACTAGCCT109050.14759739059182697No Hit
TTCAAGTAATCCAGGATAGGC106530.144186611827119No Hit
TCGTTTCCCGGCCAATGCACCA101100.13683719567935537No Hit
TACCACCCTGAACGCGCCCGA100060.13542957269709496No Hit
TGATGTAGTAGGTTGTATAGTT97080.1313961914594641No Hit
CAAAGAATTCTCCTTTTGGGCT94740.12822903974937813No Hit
AACCGGGCGGAAACACCA94130.12740341473093691No Hit
TTCACAGTGGCTAAGTTCCG91900.12438514622089773No Hit
TTCACAGTGGCTAAGTTCTGC88650.11998632440133389No Hit
CACCCGGCTGTGTGCACATGTGC88250.11944493094661833No Hit
GTGCGGTAACGCGACCGATCCCGGA87720.11872758461912024No Hit
TACGCCTGTCTGAGCGTCGCT84790.11476187756332883No Hit
TCCCACTGCTTCACTTGACTAG83540.11307002301734273No Hit
CACTAGATTGTGAGCTCCTGGA82740.11198723610791163No Hit
GGAATACCGGGTGCTGTAGGCTT81760.11066082214385854No Hit
TTCAAGTAATCCAGGATAGGCTT79960.10822455159763854No Hit
TGTAAACATCCCCGACTGGAAGC79860.10808920323395967No Hit
GGAATACCGGGTGCTGTAGGCTTT78270.10593716425146533No Hit
TGAGAACTGAATTCCATAGGCTGT77720.10519274825123147No Hit
CGCGACCTCAGATCAGACGTGGC76080.1029730350868977No Hit
TGAGGTAGTAGGTTGTATAGT76030.10290536090505825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)