FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003948000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5960271
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG76633412.857368398181896No Hit
CAACGGAATCCCAAAAGCAGCTG4096806.873512966105065No Hit
CCCCCACTGCTAAATTTGACTG2658934.461089101485486No Hit
TTCAAGTAATCCAGGATAGGCT1786082.9966422667694137No Hit
AACATTCAACGCTGTCGGTGAGT1706352.862873181437555No Hit
AACATTCAACGCTGTCGGTGAG1397762.3451282668187403No Hit
TAGCAGCACGTAAATATTGGCG1203452.0191196004342755No Hit
AAGCTGCCAGTTGAAGAACTGT1079321.8108572580005173No Hit
TATTGCACTTGTCCCGGCCTGTAA634751.0649683546268283No Hit
TAGCTTATCAGACTGATGTTGAC619961.0401540466868033No Hit
TATTGCACTTGTCCCGGCCTGTAT599981.0066320809909481No Hit
TGAGGTAGTAGGTTGTATAGTT545700.9155623964078143No Hit
TAGCTTATCAGACTGATGTTGACT527600.885194649706364No Hit
TATTGCACTTGTCCCGGCCTGT491160.8240564900488585No Hit
TAGCTTATCAGACTGATGTTGA406810.6825360793158567No Hit
TGTAAACATCCTTGACTGGAAGCT376170.6311290208113021No Hit
TATTGCACTTGTCCCGGCCTGTA355070.595727945927291No Hit
TTCTCACTACTGCACTTGACTAG310690.5212682443466077No Hit
CCCCCACTGCTAAATTTGACTGGCTT288640.48427328220478566No Hit
TTCAAGTAATTCAGGATAGGTT288570.4841558378805259No Hit
CTCCCACTGCTTCACTTGACTAG287420.48222639541054424No Hit
CATAAAGTAGAAAGCACTACT281790.4727805161879385No Hit
GCCTGTCTGAGCGTCGCT276000.4630661927955961No Hit
CAACGGAATCCCAAAAGCAGCT272790.4576805316402559No Hit
TGAGGTAGTAGATTGTATAGTT270760.45427464623672315No Hit
TTAATGCTAATCGTGATAGGGGTT268200.4499795395209379No Hit
ACCGGGTGCTGTAGGCTT261430.438620995588959No Hit
AGCAGCATTGTACAGGGCTATGA246020.41276646649120485No Hit
CAACGGAATCCCAAAAGCAGCTGT236530.39684437167370407No Hit
TGTAAACATCCCCGACTGGAAGCT224270.3762748371676389No Hit
GGAATACCGGGTGCTGTAGGCTTT204500.343105204444563No Hit
ACTGGACTTGGAGTCAGAAGGC198230.33258554854301087No Hit
ACCGGGTGCTGTAGGCTTT185990.3120495695581627No Hit
ACCGGGTGCTGTAGGCTTA185790.311714014345992No Hit
CCCCCCACTGCTAAATTTGACTGG182640.30642901975430314No Hit
CCCATAAAGTAGAAAGCACTAC178100.29881191643802774No Hit
CATTGCACTTGTCTCGGTCTGA173580.2912283686429694No Hit
ACCCCCCACTGCTAAATTTGACTG172640.289651259145767No Hit
GGAATACCGGGTGCTGTAGGCTT169190.2838629317358221No Hit
TCGTACGACTCTTAGCGG168450.2826213774507904No Hit
CCCCCCACTGCTAAATTTGACTGGCT163700.27465194116173575No Hit
CTTCTCACTACTGCACTTGACTAG158510.26594428340590553No Hit
CTCCCACTGCTTCACTTGACTAGCCT135720.2277077669790518No Hit
TGAGAACTGAATTCCATGGGTT122160.20495712359387686No Hit
AACATTCAACGCTGTCGGTGAGTT117170.19658502105021733No Hit
CGCGACCTCAGATCAGACGTGGCG115650.19403480143771987No Hit
AGCTCGGTCTGAGGCCCCTCAGT110730.1857801432183201No Hit
GGGAATACCGGGTGCTGTAGGCTT109940.18445470013024576No Hit
AACGGAATCCCAAAAGCAGCTG108920.18274336854817508No Hit
CCCATAAAGTAGAAAGCACT107070.1796394828355959No Hit
CAAAGAATTCTCCTTTTGGGCT106730.17906903897490567No Hit
TGAGGTAGTAGTTTGTACAGTT105690.17732415187161793No Hit
GTGCGGTAACGCGACCGATCCCGGA102620.17217337936479732No Hit
CGCCTGTCTGAGCGTCGCT101220.1698244928796023No Hit
AACCGGGCGGAAACACCA97080.16287849998766835No Hit
CGCGACCTCAGATCAGACGTGG96320.16160339018141961No Hit
TCGTTTCCCGGCCAATGCACCA94580.15868405983553432No Hit
TATTGCACTTGTCCCGGCCTGTT93730.15725795018380875No Hit
TGTAAACATCCTACACTCTCAGCT93000.15603317365938563No Hit
CAACACCAGTCGATGGGCTGT92050.1544392864015747No Hit
TAGCAGCACGTAAATATTGGC90690.1521575109588138No Hit
CCCCCCACTGCTAAATTTGACTGTA89840.1507314013070882No Hit
TAGCTTATCAGACTGATGTTGACA89720.1505300681797858No Hit
GGGAATACCGGGTGCTGTAGGCTTT85840.14402029706367378No Hit
TCCCACTGCTTCACTTGACTAG83880.14073185598440072No Hit
TGAGGTAGTAGTTTGTGCTGTT80710.13541330587149475No Hit
CTACGCCTGTCTGAGCGTCGCT78120.13106786587388394No Hit
CACTAGATTGTGAGCTCCTGGA77900.13069875514049614No Hit
CCCCCCACTGCTAAATTTGACTGT76510.1283666464159096No Hit
TTCAAGTAATCCAGGATAGGCTT76150.1277626470340023No Hit
TATTGCACTTGTCCCGGCCTGTAG75430.12655464827018773No Hit
TGAGGTAGTAGGTTGTGTGGTT73020.1225112079635305No Hit
CACCCGGCTGTGTGCACATGTGC72610.12182331977858053No Hit
TTCAAGTAATCCAGGATAGGC72590.12178976425736346No Hit
CAACGGAATCCCAAAAGCAGCTGA70200.11777987947192334No Hit
CACTAGATTGTGAGCTCCTGGAA69230.11615243669289534No Hit
CCCCCACTGCTAAATTTGACTGGCT65430.10977688766165161No Hit
AATGCACCCGGGCAAGGATTCT63620.10674011299150658No Hit
CAAAGAATTCTCCTTTTGGGCTT63000.10569989183377736No Hit
GACCCGCGGGCGCTCTCCAGTCCT62110.10420667113961765No Hit
TTCACAGTGGCTAAGTTCTGC61670.10346844967284206No Hit
TTCACAGTGGCTAAGTTCCG61620.10338456086979937No Hit
TGTAAACATCCCCGACTGGAAGC60280.10113634094825555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)