FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003948001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6193518
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG99643816.088400808716468No Hit
CCCCCCACTGCTAAATTTGACTG5103658.240308658181021No Hit
CCCCCACTGCTAAATTTGACTG2323223.7510506952591403No Hit
TCGTACGACTCTTAGCGG2284053.687807155803858No Hit
TAGCAGCACGTAAATATTGGCG1984453.2040756158293235No Hit
AACATTCAACGCTGTCGGTGAGT1583842.5572542131951503No Hit
AACATTCAACGCTGTCGGTGAG1549412.5016638362881967No Hit
TAGCTTATCAGACTGATGTTGAC1528232.4674667935089554No Hit
TTCAAGTAATCCAGGATAGGCT1140701.841764244489158No Hit
TAGCTTATCAGACTGATGTTGA967911.5627790215512412No Hit
AAGCTGCCAGTTGAAGAACTGT940461.518458491603641No Hit
TGAGGTAGTAGGTTGTATAGTT528790.853779709690034No Hit
TAGCTTATCAGACTGATGTTGACT488320.7884372016033536No Hit
CATAAAGTAGAAAGCACTACT445870.7198978028319284No Hit
TATTGCACTTGTCCCGGCCTGT435150.7025893845791681No Hit
AGCAGCATTGTACAGGGCTATGA370140.5976248070967098No Hit
CTCCCACTGCTTCACTTGACTAGCCT360570.5821731687871093No Hit
CCCCCACTGCTAAATTTGACTGGCTT337590.5450698617490093No Hit
CATTGCACTTGTCTCGGTCTGA325270.5251780974883742No Hit
ACCGGGTGCTGTAGGCTT256430.41402963549956584No Hit
TTCACAGTGGCTAAGTTCCGC251970.4068285585026151No Hit
TAGCTTATCAGACTGATGTTGACA247390.3994337305550739No Hit
TAGCAGCACGTAAATATTGGC233430.3768940366363673No Hit
TATTGCACTTGTCCCGGCCTGTA212920.3437787699979236No Hit
ACTGGACTTGGAGTCAGAAGGC210970.34063031705082636No Hit
CAACACCAGTCGATGGGCTGT193250.31201976001361426No Hit
CAACGGAATCCCAAAAGCAGCT187760.3031556540240942No Hit
TGTAAACATCCTTGACTGGAAGCT177020.2858149439462354No Hit
TTCAAGTAATTCAGGATAGGTT173290.27979251856537757No Hit
AACGGAATCCCAAAAGCAGCTG171090.27624041780454983No Hit
TGAGGTAGTAGATTGTATAGTT158130.25531531514076494No Hit
AACATTCAACGCTGTCGGTGAGTT154840.2500033099120726No Hit
TGAGGTAGTAGTTTGTGCTGTT153770.24827569726930643No Hit
TTCACCACCTTCTCCACCCAGC142800.23056363120281556No Hit
TAGCAGCACGTAAATATTGGCGT138850.22418599574587494No Hit
TGAGAACTGAATTCCATGGGTT134920.21784065211403278No Hit
ACCGGGTGCTGTAGGCTTT134040.21641981180970168No Hit
TGTAAACATCCTACACTCTCAGCT123190.19890149669380147No Hit
CGCGACCTCAGATCAGACGTGG119280.19258844488705773No Hit
CAACGGAATCCCAAAAGCAGCTGT116290.18776081703484193No Hit
TGTAAACATCCCCGACTGGAAGCT116190.18759935790934973No Hit
GTGCGGTAACGCGACCGATCCCGGA113160.18270714640693705No Hit
CCCATAAAGTAGAAAGCACT112790.18210974764261603No Hit
TTCACAGTGGCTAAGTTCTGC105640.17056542016992604No Hit
TGAGGTAGTAGGTTGTGTGGTT105520.17037166921933544No Hit
TCCCACTGCTTCACTTGACTAGCCT103720.1674654049604764No Hit
AGCAGCATTGTACAGGGCTATG103590.1672555080973366No Hit
TATTGCACTTGTCCCGGCCTGTAT102890.16612529421889144No Hit
ACCCCCCACTGCTAAATTTGACTG100850.1628315280588512No Hit
CCCCCCACTGCTAAATTTGACTGG96560.15590493157523722No Hit
CCCCCCACTGCTAAATTTGACTGGCT89880.1451194619923604No Hit
CCCATAAAGTAGAAAGCACTAC86240.1392423498244455No Hit
CACTAGATTGTGAGCTCCTGGA84450.1363522314781357No Hit
TCAGTGCATCACAGAACTTTGT82780.13365586408241648No Hit
TATTGCACTTGTCCCGGCCTGTAA81880.13220273195298698No Hit
AGCAGCATTGTACAGGGCTATGT79390.128182399728232No Hit
CTTCTCACTACTGCACTTGACTAG76440.12341935552621305No Hit
TATTGCACTTGTCCCGGCCTGTT75750.1223052875603171No Hit
GTGGGTGGTGGTGCATGGCCG73980.11944746103910572No Hit
TGATGTAGTAGGTTGTATAGTT73500.1186724572367433No Hit
ACCACAGGGTAGAACCACGGAC70730.11420003946061028No Hit
CACTAGATTGTGAGCTCCTGGAA69790.1126823236809839No Hit
CGCGACCTCAGATCAGACGTG68340.11034116636134746No Hit
TTCACAGTGGCTAAGTTCCG65850.10632083413659249No Hit
CACCCGGCTGTGTGCACATGTGC65730.10612708318600188No Hit
ATCCCACCGCTGCCACCA63920.10320467301459363No Hit
CTCCCACTGCTTCACTTGACTAG63350.10228435599928828No Hit
CAAAGAATTCTCCTTTTGGGCT63100.10188070818555788No Hit
TAGCTTATCAGACTGATGTTG62660.10117028803339233No Hit
CTCGTACGACTCTTAGCGG62620.10110570438319547No Hit
CCCATAAAGTAGAAAGCACTA62350.10066976474436662No Hit
CAAAGTGCTGTTCGTGCAGGTAG62110.10028226284318541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)