FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003948002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5968791
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5003858.38335602637117No Hit
CAACGGAATCCCAAAAGCAGCTG4120986.904212260070758No Hit
GCCTGTCTGAGCGTCGCT2788614.671984661550388No Hit
AACATTCAACGCTGTCGGTGAGT1923463.2225286494367116No Hit
CCCCCACTGCTAAATTTGACTG1525322.5554923936857565No Hit
TTCAAGTAATCCAGGATAGGCT1343062.2501374231397953No Hit
AAGCTGCCAGTTGAAGAACTGT1265102.1195247077674524No Hit
AACATTCAACGCTGTCGGTGAG1162781.9480997072941573No Hit
TAGCAGCACGTAAATATTGGCG1009691.6916156052373086No Hit
TGAGGTAGTAGGTTGTATAGTT669701.1220027640438408No Hit
TATTGCACTTGTCCCGGCCTGTAA655991.0990332883158416No Hit
TATTGCACTTGTCCCGGCCTGT547620.9174722318137793No Hit
TCGTACGACTCTTAGCGG547270.9168858484071565No Hit
TATTGCACTTGTCCCGGCCTGTAT546230.9151434519989057No Hit
CGCCTGTCTGAGCGTCGCT511040.8561867889158793No Hit
TCCTGTCTGAGCGTCGCT510310.85496376066778No Hit
TAGCTTATCAGACTGATGTTGAC432170.7240494766863173No Hit
TAGCTTATCAGACTGATGTTGACT373250.6253360186342595No Hit
TGAGGTAGTAGATTGTATAGTT326070.5462915354214949No Hit
CGCGACCTCAGATCAGACGTGG299970.5025640870990457No Hit
ACCGGGTGCTGTAGGCTT299510.501793411764627No Hit
TATTGCACTTGTCCCGGCCTGTA293180.4911882490105618No Hit
CGCGACCTCAGATCAGACGTGGCG279770.4687213876310965No Hit
TGTAAACATCCTTGACTGGAAGCT269520.45154873072285495No Hit
TTCAAGTAATTCAGGATAGGTT265050.4440597769296999No Hit
TAGCTTATCAGACTGATGTTGA262670.44007236976466424No Hit
ACTGGACTTGGAGTCAGAAGGC262550.43987132402525064No Hit
CAACGGAATCCCAAAAGCAGCTGT262030.4390001258211253No Hit
CACCCGGCTGTGTGCACATGTGC256150.42914888458986084No Hit
CATAAAGTAGAAAGCACTACT248090.41564531242591674No Hit
AGCAGCATTGTACAGGGCTATGA243760.4083909119954108No Hit
CTCCCACTGCTTCACTTGACTAG227460.38108219905840224No Hit
ACCGGGTGCTGTAGGCTTA214380.3591682134623243No Hit
ACCGGGTGCTGTAGGCTTT207610.34782588299707595No Hit
AACCGGGCGGAAACACCA180510.3024230535128471No Hit
TGTAAACATCCCCGACTGGAAGCT165570.2773928589558589No Hit
TTCTCACTACTGCACTTGACTAG157070.2631521190807318No Hit
CAACGGAATCCCAAAAGCAGCT155000.25968408007584787No Hit
TACGCCTGTCTGAGCGTCGCT142880.23937846039507835No Hit
CTACGCCTGTCTGAGCGTCGCT141230.23661408147814192No Hit
AGCTCGGTCTGAGGCCCCTCAGT140350.2351397460557758No Hit
CATTGCACTTGTCTCGGTCTGA138900.23271044337119526No Hit
CCCATAAAGTAGAAAGCACTAC128380.2150854335492732No Hit
AACATTCAACGCTGTCGGTGAGTT127880.2142477429683834No Hit
CCCCCCACTGCTAAATTTGACTGG125550.21034410486143676No Hit
CGCGACCTCAGATCAGACGTGGC124940.20932212235275116No Hit
TACCACCCTGAACGCGCCCGA124490.2085682008299503No Hit
TGAGGTAGTAGTTTGTACAGTT124430.2084676779602435No Hit
ACCCCCCACTGCTAAATTTGACTG123290.20655774343581473No Hit
CCCCCACTGCTAAATTTGACTGGCTT121590.2037095954607893No Hit
AACGGAATCCCAAAAGCAGCTG118300.19819759143853422No Hit
ACGCCTGTCTGAGCGTCGCT116580.19531593584027318No Hit
TGAGGTAGTAGGTTGTGTGGTT112170.18792750491682486No Hit
CGCGACCTCAGATCAGACGTG106890.17908149238262824No Hit
CAACACCAGTCGATGGGCTGT93850.15723452203302143No Hit
CCCATAAAGTAGAAAGCACT93780.15711724535169685No Hit
ACCACCCTGAACGCGCCCGA92410.15482197316005872No Hit
CTTCTCACTACTGCACTTGACTAG90730.1520073328082689No Hit
CCACCCTGAACGCGCCCGA90360.1513874417784104No Hit
CCCCCCACTGCTAAATTTGACTGGCT88840.14884086241250533No Hit
TATTGCACTTGTCCCGGCCTGTT86090.14423356421761124No Hit
GGAATACCGGGTGCTGTAGGCTTT80560.1349687063929697No Hit
TGAGGTAGTAGTTTGTGCTGTT80320.13456661491414257No Hit
GGAATACCGGGTGCTGTAGGCTT78560.13161794406941035No Hit
CAACGGAATCCCAAAAGCAGCTGA77780.13031114676322222No Hit
TTCAAGTAATCCAGGATAGGCTT75570.1266085543956892No Hit
ATCCCGGACGAGCCCCCA74740.12521798803141207No Hit
CACTAGATTGTGAGCTCCTGGA74290.1244640665086112No Hit
CCCCCCACTGCTAAATTTGACTGT74140.12421275933434425No Hit
CGCGACCTCAGATCAGACGT73790.12362637592772138No Hit
TACCACCCTGAACGCGCCCGAT72570.1215824109103502No Hit
CCCCCCACTGCTAAATTTGACTGTA72400.12129759611284766No Hit
GGGAATACCGGGTGCTGTAGGCTT71970.12057718221328238No Hit
TGTAAACATCCTACACTCTCAGCT71640.1200243064298951No Hit
TCGTTTCCCGGCCAATGCACCA71310.11947143064650781No Hit
TATTGCACTTGTCCCGGCCTGTAG69660.11670705172957137No Hit
GCGTTCGCGCTTTCCCCTG68970.1155510387279434No Hit
CTCCCACTGCTTCACTTGACTAGCCT67470.1130379669852739No Hit
GTGCGGTAACGCGACCGATCCCGGA67270.11270289075291796No Hit
CCCTGTCTGAGCGTCGCT66490.11139609344672982No Hit
TTCAAGTAATCCAGGATAGGC65650.10998877327083492No Hit
TTCACAGTGGCTAAGTTCTGC63540.10645371901947982No Hit
TAGCAGCACGTAAATATTGGC63140.10578356655476795No Hit
AATGCACCCGGGCAAGGATTCT62840.10528095220623407No Hit
AGAGGTAGTAGGTTGCATAGTT61940.10377310916063237No Hit
CACTAGATTGTGAGCTCCTGGAA61000.10219825086855948No Hit
TACCACCCTGAACGCGCCCG60440.10126003741796287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)