FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003948003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9844782
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5928356.021819477566898No Hit
GCCTGTCTGAGCGTCGCT5388955.473915014065319No Hit
CAACGGAATCCCAAAAGCAGCTG4685184.759049006874911No Hit
AACATTCAACGCTGTCGGTGAGT2776682.820458594207571No Hit
AAGCTGCCAGTTGAAGAACTGT2695922.738425289661061No Hit
TTCAAGTAATCCAGGATAGGCT2285112.321138243589345No Hit
CCCCCACTGCTAAATTTGACTG1892431.9222670446130752No Hit
TAGCAGCACGTAAATATTGGCG1865961.895379704700419No Hit
AACATTCAACGCTGTCGGTGAG1509481.5332792539235505No Hit
GGAATACCGGGTGCTGTAGGCTTT1104791.1222086989838882No Hit
TAGCTTATCAGACTGATGTTGAC1070721.0876015334824072No Hit
TATTGCACTTGTCCCGGCCTGT1037871.0542336031412378No Hit
TATTGCACTTGTCCCGGCCTGTAA1023051.0391799432430298No Hit
TGAGGTAGTAGGTTGTATAGTT914990.9294162125682417No Hit
TATTGCACTTGTCCCGGCCTGTAT914880.9293044782505087No Hit
CGCCTGTCTGAGCGTCGCT914220.9286340723441108No Hit
GGAATACCGGGTGCTGTAGGCTT876200.8900146290694908No Hit
AACCGGGCGGAAACACCA838740.8519640150487843No Hit
TAGCTTATCAGACTGATGTTGACT802080.8147260142479539No Hit
TCGTACGACTCTTAGCGG689760.7006351181773247No Hit
TGTAAACATCCTTGACTGGAAGCT688910.6997717166312063No Hit
AGCAGCATTGTACAGGGCTATGA584770.5939897907338121No Hit
TAGCTTATCAGACTGATGTTGA575490.5845634773832473No Hit
TATTGCACTTGTCCCGGCCTGTA546810.5554312934506829No Hit
TGAGGTAGTAGATTGTATAGTT502120.5100366874553444No Hit
TCCTGTCTGAGCGTCGCT486240.49390631504080035No Hit
CATAAAGTAGAAAGCACTACT442190.4491617996213629No Hit
ACCGGGTGCTGTAGGCTT428290.4350426449260126No Hit
TTCAAGTAATTCAGGATAGGTT427610.43435192368911774No Hit
ACTGGACTTGGAGTCAGAAGGC419710.4263273681428395No Hit
CGCGACCTCAGATCAGACGTGG376790.3827306688964774No Hit
CGCGACCTCAGATCAGACGTGGCG370460.3763008667942063No Hit
ACCGGGTGCTGTAGGCTTA368040.3738427118040806No Hit
TGTAAACATCCCCGACTGGAAGCT361640.3673418060450704No Hit
TTAATGCTAATCGTGATAGGGGTT328870.33405513702588846No Hit
CTCCCACTGCTTCACTTGACTAG318090.3231051738880556No Hit
CTACGCCTGTCTGAGCGTCGCT318070.32308485855755875No Hit
ACCGGGTGCTGTAGGCTTT309400.3142781627871496No Hit
CAACGGAATCCCAAAAGCAGCTGT305930.31075345294593626No Hit
TACGCCTGTCTGAGCGTCGCT304190.30898601919270535No Hit
CACCCGGCTGTGTGCACATGTGC281640.2860804840574428No Hit
CATTGCACTTGTCTCGGTCTGA280060.28447557294818715No Hit
TTCTCACTACTGCACTTGACTAG276450.28080865579349545No Hit
CCCATAAAGTAGAAAGCACTAC258410.26248422768528545No Hit
ATCCCGGACGAGCCCCCA242680.24650622024946822No Hit
ACGCCTGTCTGAGCGTCGCT233190.23686659592868586No Hit
AACATTCAACGCTGTCGGTGAGTT231320.23496711252722507No Hit
GCGTTCGCGCTTTCCCCTG221250.22473834362203246No Hit
GGAATACCGGGTGCTGTAGGCTTA209220.21251867232814298No Hit
TGTAAACATCCTACACTCTCAGCT204840.2080696149493204No Hit
TGAGGTAGTAGTTTGTACAGTT195150.198226837323569No Hit
AGCTCGGTCTGAGGCCCCTCAGT184230.1871346668722578No Hit
GGGCTACGCCTGTCTGAGCGTCGCT178780.18159873931185067No Hit
CCCATAAAGTAGAAAGCACT167670.1703135732208189No Hit
CTTCTCACTACTGCACTTGACTAG164360.1669513860235808No Hit
CCCCCCACTGCTAAATTTGACTGG159610.16212649503056542No Hit
TATTGCACTTGTCCCGGCCTGTT154770.15721018505031398No Hit
CGCGACCTCAGATCAGACGTGGC153020.1554325936318346No Hit
CCCCCACTGCTAAATTTGACTGGCTT150670.15304554229844805No Hit
ACCCCCCACTGCTAAATTTGACTG145470.14776355636925226No Hit
GGAATACCGGGTGCTGTAGGCT144990.14727598843732648No Hit
CAACACCAGTCGATGGGCTGT144260.1465344788741894No Hit
TGAGGTAGTAGTTTGTGCTGTT141040.1432637106641874No Hit
TTCAAGTAATCCAGGATAGGCTT140850.14307071502446678No Hit
CGCGACCTCAGATCAGACGTG136630.13878418028961942No Hit
GTGCGGTAACGCGACCGATCCCGGA133490.13559467340160505No Hit
TGAGGTAGTAGGTTGTGTGGTT131290.13335998704694527No Hit
CAACGGAATCCCAAAAGCAGCT128280.13030252980716078No Hit
AACGGAATCCCAAAAGCAGCTG127970.12998764218445874No Hit
TAGCTTATCAGACTGATGTTGACA127730.12974385821849585No Hit
TACCACCCTGAACGCGCCCGA123360.1253049585049217No Hit
TGAATACCGGGTGCTGTAGGCTTT123270.12521353951768563No Hit
AGCAGCATTGTACAGGGCTATGT122590.12452281828079079No Hit
ACCACAGGGTAGAACCACGGAC121760.12367973206516913No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT121660.1235781554126846No Hit
TTATAAAGCAATGAGACTGATT119670.12155678002824237No Hit
CCCCCCACTGCTAAATTTGACTGGCT113820.11561454585789711No Hit
GGGAATACCGGGTGCTGTAGGCTT112410.11418231505786516No Hit
TTCACAGTGGCTAAGTTCCG112220.11398931941814455No Hit
TATTGCACTTGTCCCGGCCTGTAG109070.11078965486488172No Hit
TTCAAGTAATCCAGGATAGGC108360.11006846063224152No Hit
CAAAGTGCTGTTCGTGCAGGTAG107760.10945900071733432No Hit
CGCGACCTCAGATCAGACGT107470.10916442842512916No Hit
CCCCCCACTGCTAAATTTGACTGTA107380.1090730094378931No Hit
TAGCAGCACGTAAATATTGGC107310.10900190578115392No Hit
CTCGACACAAGGGTTTGT107280.10897143278540855No Hit
TGTCAGTTTGTCAAATACCCCAA106440.10811818890453848No Hit
TGATGTAGTAGGTTGTATAGTT106160.10783377427758176No Hit
CTCCCACTGCTTCACTTGACTAGCCT104640.10628980915981685No Hit
CCCCCCACTGCTAAATTTGACTGT103310.10493883968177255No Hit
CCTCGACACAAGGGTTTGT102420.1040348074746602No Hit
GAATACCGGGTGCTGTAGGCTTT101890.10349645121649216No Hit
CACTAGATTGTGAGCTCCTGGA100230.10181027878524887No Hit
TGTAAACATCCCCGACTGGAAGC99900.10147507583204991No Hit
CAAAGAATTCTCCTTTTGGGCT99200.10076403926465817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)