FastQCFastQC Report
Thu 8 Sep 2022
EGAF00003948004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6707953
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6361809.483966271081506No Hit
CCCCCCACTGCTAAATTTGACTG5921488.827551415461617No Hit
CCCCCACTGCTAAATTTGACTG2212073.297682616440515No Hit
AACATTCAACGCTGTCGGTGAGT2131813.1780335968364715No Hit
TTCAAGTAATCCAGGATAGGCT1935062.8847250420508312No Hit
TAGCAGCACGTAAATATTGGCG1713672.5546839699085546No Hit
AAGCTGCCAGTTGAAGAACTGT1608802.3983471559803715No Hit
AACATTCAACGCTGTCGGTGAG1439682.146228514123459No Hit
GCCTGTCTGAGCGTCGCT1284821.9153682203796003No Hit
TCGTACGACTCTTAGCGG953551.4215215878823242No Hit
TGAGGTAGTAGGTTGTATAGTT824451.2290634713749484No Hit
TATTGCACTTGTCCCGGCCTGTAA754651.1250078824344776No Hit
TATTGCACTTGTCCCGGCCTGTAT637950.951035286025409No Hit
TATTGCACTTGTCCCGGCCTGT621070.9258711264077133No Hit
TAGCTTATCAGACTGATGTTGAC534540.7968749930120261No Hit
CATAAAGTAGAAAGCACTACT530920.7914784137575204No Hit
TAGCTTATCAGACTGATGTTGACT517030.7707716497119166No Hit
TAGCTTATCAGACTGATGTTGA444810.6631084028167759No Hit
CAACGGAATCCCAAAAGCAGCTGT383300.571411278522673No Hit
TGTAAACATCCTTGACTGGAAGCT376560.5613635038885931No Hit
TATTGCACTTGTCCCGGCCTGTA362490.5403884016480139No Hit
TGAGGTAGTAGATTGTATAGTT347410.5179076239800726No Hit
CACCCGGCTGTGTGCACATGTGC292420.4359303054150797No Hit
TTCAAGTAATTCAGGATAGGTT283920.42325877954124014No Hit
AGCAGCATTGTACAGGGCTATGA283820.42310970276625376No Hit
ACCGGGTGCTGTAGGCTT277900.4142843576870619No Hit
CCCATAAAGTAGAAAGCACTAC276540.4122569135472476No Hit
ACTGGACTTGGAGTCAGAAGGC244320.36422437664664614No Hit
TGTAAACATCCCCGACTGGAAGCT233210.3476619469456629No Hit
TTAATGCTAATCGTGATAGGGGTT228140.340103754453855No Hit
CCCCCACTGCTAAATTTGACTGGCTT216130.32219963377799454No Hit
TGAGAACTGAATTCCATGGGTT206160.30733667931185565No Hit
CATTGCACTTGTCTCGGTCTGA203540.3034308678072133No Hit
CAACGGAATCCCAAAAGCAGCT188460.28095009013927197No Hit
TTCTCACTACTGCACTTGACTAG185710.2768504788271474No Hit
CCCATAAAGTAGAAAGCACT181570.2706787003427126No Hit
ACCGGGTGCTGTAGGCTTT178870.2666536274180812No Hit
CGCCTGTCTGAGCGTCGCT176720.2634484767558747No Hit
ACCGGGTGCTGTAGGCTTA168380.25101547372201327No Hit
CGCGACCTCAGATCAGACGTGG165690.2470053084748805No Hit
CTCCCACTGCTTCACTTGACTAG160620.23944711598307264No Hit
CAACACCAGTCGATGGGCTGT148580.22149827227471627No Hit
TGAGGTAGTAGTTTGTACAGTT148560.22146845691971903No Hit
ACCCCCCACTGCTAAATTTGACTG145060.21625076979519686No Hit
AACGGAATCCCAAAAGCAGCTG145040.21622095444019956No Hit
TGATGTAGTAGGTTGTATAGTT142990.2131648805529794No Hit
AACATTCAACGCTGTCGGTGAGTT134610.20067224680912343No Hit
CGCGACCTCAGATCAGACGTGGCG131760.19642355872201253No Hit
TAGCAGCACGTAAATATTGGC121240.18074068199344867No Hit
CCCCCCACTGCTAAATTTGACTGG119310.17786350023621217No Hit
TCGTTTCCCGGCCAATGCACCA118000.175910594483891No Hit
ATCCCGGACGAGCCCCCA115110.17160227568678552No Hit
CTTCTCACTACTGCACTTGACTAG112910.16832258663708585No Hit
TGTAAACATCCTACACTCTCAGCT107620.16043642524030802No Hit
TACCACCCTGAACGCGCCCGA107110.15967613368787764No Hit
CACTAGATTGTGAGCTCCTGGA105750.15764868954806333No Hit
TATTGCACTTGTCCCGGCCTGTT103440.1542050160458787No Hit
CTCCCACTGCTTCACTTGACTAGCCT101940.15196886442108345No Hit
TGAGGTAGTAGTTTGTGCTGTT96880.14442557960677424No Hit
TGAGAACTGAATTCCATAGGCTGT96360.14365038037684522No Hit
CAAAGAATTCTCCTTTTGGGCT96010.143128611664393No Hit
CTACGCCTGTCTGAGCGTCGCT93870.13993836867968515No Hit
AGCTCGGTCTGAGGCCCCTCAGT93360.13917807712725475No Hit
CGCGACCTCAGATCAGACGTGGC93030.1386861237697998No Hit
CCCCCCACTGCTAAATTTGACTGGCT91920.13703137156745135No Hit
AACCGGGCGGAAACACCA91660.13664377195248684No Hit
TTCACAGTGGCTAAGTTCCGC90730.13525735794511382No Hit
TGAGGTAGTAGGTTGTGTGGTT89620.13360260574276533No Hit
TTCAAGTAATCCAGGATAGGC87170.1299502247555998No Hit
CACTAGATTGTGAGCTCCTGGAA87160.1299353170781012No Hit
GGAATACCGGGTGCTGTAGGCTT85560.1275500886783196No Hit
TATTGCACTTGTCCCGGCCTGTAG80750.12037949580147626No Hit
GTGCGGTAACGCGACCGATCCCGGA80540.12006643457400491No Hit
TTCAAGTAATCCAGGATAGGCTT79450.11844149772665372No Hit
GGAATACCGGGTGCTGTAGGCTTT78170.11653331500682845No Hit
CCACCCTGAACGCGCCCGA75400.11240388833970662No Hit
TCCTGTCTGAGCGTCGCT74510.11107710504232812No Hit
TTCACAGTGGCTAAGTTCTGC72900.10867696896504792No Hit
TAGCTTATCAGACTGATGTTGACA72480.10805084651010526No Hit
CCCCCCACTGCTAAATTTGACTGT71880.10715638586018715No Hit
ACCACCCTGAACGCGCCCGA70400.1049500495903892No Hit
CCCATAAAGTAGAAAGCACTA69830.10410031197296701No Hit
CCCCCCACTGCTAAATTTGACTGTA69720.10393632752048203No Hit
TGTAAACATCCCCGACTGGAAGC68630.10231139067313083No Hit
CAAAGAATTCTCCTTTTGGGCTT67730.10096969969825369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)