FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003948005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9754269
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG106999010.969453477241606No Hit
CAACGGAATCCCAAAAGCAGCTG7301147.485071408221364No Hit
CCCCCACTGCTAAATTTGACTG3777913.873083672390007No Hit
TTCAAGTAATCCAGGATAGGCT2916232.98969610126602No Hit
TAGCAGCACGTAAATATTGGCG2644042.710649050174852No Hit
AACATTCAACGCTGTCGGTGAGT2278962.3363719003443517No Hit
AAGCTGCCAGTTGAAGAACTGT2146702.2007799866909554No Hit
AACATTCAACGCTGTCGGTGAG1838391.8847029951706273No Hit
GCCTGTCTGAGCGTCGCT1067831.0947309326818853No Hit
TATTGCACTTGTCCCGGCCTGTAA1027231.0531081314243025No Hit
TATTGCACTTGTCCCGGCCTGTAT1021951.0476951168765185No Hit
TGAGGTAGTAGGTTGTATAGTT990421.0153708084121935No Hit
TAGCTTATCAGACTGATGTTGAC966910.9912685409844654No Hit
TATTGCACTTGTCCCGGCCTGT894790.91733168318405No Hit
TAGCTTATCAGACTGATGTTGACT819960.8406165546592984No Hit
TAGCTTATCAGACTGATGTTGA678310.6953980867248996No Hit
TATTGCACTTGTCCCGGCCTGTA639260.6553643332985793No Hit
TGTAAACATCCTTGACTGGAAGCT638420.65450317189325No Hit
TTAATGCTAATCGTGATAGGGGTT580580.5952060579834326No Hit
CATAAAGTAGAAAGCACTACT528920.5422446315556809No Hit
TTCAAGTAATTCAGGATAGGTT499790.5123807842494399No Hit
TGAGGTAGTAGATTGTATAGTT494900.5073675946398444No Hit
CAACGGAATCCCAAAAGCAGCT491390.503769170196147No Hit
AGCAGCATTGTACAGGGCTATGA451710.46308954571582966No Hit
CAACGGAATCCCAAAAGCAGCTGT426470.43721369586998265No Hit
ACCGGGTGCTGTAGGCTT375530.384990407789656No Hit
TTCTCACTACTGCACTTGACTAG373090.3824889389456042No Hit
CCCCCACTGCTAAATTTGACTGGCTT359960.36902816602658794No Hit
TGTAAACATCCCCGACTGGAAGCT348830.3576177774059748No Hit
CTCCCACTGCTTCACTTGACTAG345850.35456270480135416No Hit
CGCGACCTCAGATCAGACGTGG332460.3408353819235455No Hit
CACCCGGCTGTGTGCACATGTGC324160.33232628708517264No Hit
ACCGGGTGCTGTAGGCTTA316680.3246578498091451No Hit
ACTGGACTTGGAGTCAGAAGGC314390.32231015978747357No Hit
TGAGAACTGAATTCCATGGGTT312440.3203110350965306No Hit
CATTGCACTTGTCTCGGTCTGA301460.3090544252982976No Hit
CCCATAAAGTAGAAAGCACTAC281240.2883250400414424No Hit
AACCGGGCGGAAACACCA278350.2853622347302499No Hit
ACCGGGTGCTGTAGGCTTT277250.2842345233661282No Hit
CGCGACCTCAGATCAGACGTGGCG264630.2712965984432047No Hit
ACCCCCCACTGCTAAATTTGACTG260480.26704205102401835No Hit
GGAATACCGGGTGCTGTAGGCTT222040.22763366480871094No Hit
CTTCTCACTACTGCACTTGACTAG216880.22234367331883095No Hit
CCCCCCACTGCTAAATTTGACTGG203540.20866761004848236No Hit
GGGAATACCGGGTGCTGTAGGCTT201440.2065147065351591No Hit
GTGCGGTAACGCGACCGATCCCGGA200670.20572530858027394No Hit
GGAATACCGGGTGCTGTAGGCTTT197730.20271124366162138No Hit
TAGCAGCACGTAAATATTGGC190420.1952170890509581No Hit
TGAGGTAGTAGTTTGTACAGTT179800.18432954842643767No Hit
CCCATAAAGTAGAAAGCACT176970.18142825464419732No Hit
CTCCCACTGCTTCACTTGACTAGCCT175900.18033129904455167No Hit
AACGGAATCCCAAAAGCAGCTG175640.18006474908575926No Hit
TCGTTTCCCGGCCAATGCACCA175600.18002374139979122No Hit
TATTGCACTTGTCCCGGCCTGTT175470.17989046642039502No Hit
CAAAGAATTCTCCTTTTGGGCT175060.17947013763922237No Hit
TCGTACGACTCTTAGCGG175050.17945988571773036No Hit
TGTAAACATCCTACACTCTCAGCT169920.17420064999232643No Hit
AGCTCGGTCTGAGGCCCCTCAGT168470.17271412137598421No Hit
AACATTCAACGCTGTCGGTGAGTT166240.17042794288326474No Hit
CGCGACCTCAGATCAGACGTGGC164620.16876713160155826No Hit
CACTAGATTGTGAGCTCCTGGA156400.16034005213512156No Hit
CCCCCCACTGCTAAATTTGACTGGCT142110.1456900563230315No Hit
TGATGTAGTAGGTTGTATAGTT141950.14552602557915925No Hit
CACTAGATTGTGAGCTCCTGGAA140790.14433680268608545No Hit
CAACACCAGTCGATGGGCTGT135410.13882126892338112No Hit
TATTGCACTTGTCCCGGCCTGTAG133760.13712970187719858No Hit
GGGAATACCGGGTGCTGTAGGCTTT131040.1343411792313704No Hit
CAACGGAATCCCAAAAGCAGCTGA127520.13073250286618096No Hit
CCCCCCACTGCTAAATTTGACTGT127210.13041469329992847No Hit
TTCACAGTGGCTAAGTTCCG122340.12542200753331695No Hit
TGAGGTAGTAGTTTGTGCTGTT122120.1251964652604926No Hit
TAGCTTATCAGACTGATGTTGACA121740.12480689224379601No Hit
TGAGAACTGAATTCCATAGGCTGT120880.12392522699548268No Hit
TTCAAGTAATCCAGGATAGGCTT120060.12308456943313742No Hit
TTCACAGTGGCTAAGTTCTGC118430.1214135062299389No Hit
TATTGCACTTGTCCCGGCCTGTAC117870.12083939862638604No Hit
TTCAAGTAATCCAGGATAGGC117350.12030629870880125No Hit
CCCCCCACTGCTAAATTTGACTGTA111680.11449345922282848No Hit
AATGCACCCGGGCAAGGATTCT111510.11431917655746424No Hit
TGAGGTAGTAGGTTGTGTGGTT110380.11316070942886647No Hit
TGTAAACATCCCCGACTGGAAGC103130.10572806634715527No Hit
CTACGCCTGTCTGAGCGTCGCT102890.10548202023134691No Hit
CAAAGAATTCTCCTTTTGGGCTT102210.10478488956988986No Hit
TACCACCCTGAACGCGCCCGA102000.10456959921855753No Hit
CGCCTGTCTGAGCGTCGCT100770.10330861287503963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)