FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003948007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7355851
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG75262410.231637372752656No Hit
CAACGGAATCCCAAAAGCAGCTG5856667.9619067868557964No Hit
GCCTGTCTGAGCGTCGCT5631687.656055023409255No Hit
CCCCCACTGCTAAATTTGACTG2755763.746351034027198No Hit
AACATTCAACGCTGTCGGTGAGT2359063.2070524538901073No Hit
AAGCTGCCAGTTGAAGAACTGT1948332.648680621725481No Hit
AACATTCAACGCTGTCGGTGAG1646052.237742444755882No Hit
TCCTGTCTGAGCGTCGCT1641172.231108270137609No Hit
TTCAAGTAATCCAGGATAGGCT1582192.1509272006733147No Hit
TAGCAGCACGTAAATATTGGCG1312001.78361415966691No Hit
TGAGGTAGTAGGTTGTATAGTT1039451.4130927883123245No Hit
TAGCTTATCAGACTGATGTTGACT1038051.4111895414956066No Hit
TAGCTTATCAGACTGATGTTGAC1033431.4049088270004382No Hit
TATTGCACTTGTCCCGGCCTGTAA895601.2175341778945767No Hit
TATTGCACTTGTCCCGGCCTGTAT775861.0547521965847322No Hit
TGAGGTAGTAGATTGTATAGTT595330.8093285195689798No Hit
TATTGCACTTGTCCCGGCCTGT555040.7545557951078672No Hit
CGCCTGTCTGAGCGTCGCT550600.7485197837748481No Hit
TAGCTTATCAGACTGATGTTGA543290.7385821164675576No Hit
TGTAAACATCCTTGACTGGAAGCT489040.6648313023197452No Hit
TATTGCACTTGTCCCGGCCTGTA382950.5206059774728987No Hit
ACTGGACTTGGAGTCAGAAGGC368150.5004859396961684No Hit
AGCAGCATTGTACAGGGCTATGA359690.4889848910751455No Hit
TTCAAGTAATTCAGGATAGGTT347120.47189645358504406No Hit
CACCCGGCTGTGTGCACATGTGC312560.42491344645235474No Hit
CATAAAGTAGAAAGCACTACT312340.42461436480972764No Hit
CAACGGAATCCCAAAAGCAGCTGT289110.3930340622723326No Hit
AACCGGGCGGAAACACCA284400.3866309961960893No Hit
TGTAAACATCCCCGACTGGAAGCT281230.38232150161823564No Hit
TTCTCACTACTGCACTTGACTAG257820.3504964959186911No Hit
TGAGGTAGTAGTTTGTACAGTT241440.32822850816309357No Hit
CAACACCAGTCGATGGGCTGT230320.31311129059030696No Hit
TCGTACGACTCTTAGCGG213100.28970135474467873No Hit
GGAATACCGGGTGCTGTAGGCTTT198410.2697308577892619No Hit
CTCCCACTGCTTCACTTGACTAG176700.24021693751001752No Hit
AACGGAATCCCAAAAGCAGCTG175100.23804179829091154No Hit
CCCATAAAGTAGAAAGCACTAC173900.23641044387658205No Hit
CATTGCACTTGTCTCGGTCTGA173560.23594822679252203No Hit
AACATTCAACGCTGTCGGTGAGTT165890.22552115316093269No Hit
CTTCTCACTACTGCACTTGACTAG159180.21639916306080698No Hit
ACCCCCCACTGCTAAATTTGACTG158960.2161000814181799No Hit
CCCCCCACTGCTAAATTTGACTGG157020.2134627251150139No Hit
CCCCCACTGCTAAATTTGACTGGCTT156740.21308207575167032No Hit
TACGCCTGTCTGAGCGTCGCT149830.20368819324915635No Hit
CCCATAAAGTAGAAAGCACT147060.19992248347607913No Hit
AGCTCGGTCTGAGGCCCCTCAGT137900.18746981144669733No Hit
CCCTGTCTGAGCGTCGCT135050.1835953447126648No Hit
CTACGCCTGTCTGAGCGTCGCT131990.17943539095612457No Hit
TAGCTTATCAGACTGATGTTGACA126190.17155051128686538No Hit
GGAATACCGGGTGCTGTAGGCTT126050.17136018660519362No Hit
TGAGGTAGTAGGTTGTGTGGTT119340.16223819650506788No Hit
TGAGGTAGTAGTTTGTGCTGTT117330.15950567786106598No Hit
CAACGGAATCCCAAAAGCAGCT114200.1552505617636899No Hit
ACGCCTGTCTGAGCGTCGCT107940.1467403295689377No Hit
TAGCAGCACGTAAATATTGGC107490.14612857166356416No Hit
CCCCCCACTGCTAAATTTGACTGGCT105440.14334167453908458No Hit
ACTGGACTTGGAGTCAGAAGGCT104360.14187345556618805No Hit
TTCAAGTAATCCAGGATAGGCTT100540.13668031068057251No Hit
TATTGCACTTGTCCCGGCCTGTAG97550.1326155192648682No Hit
TATTGCACTTGTCCCGGCCTGTT96110.1306578939676728No Hit
CGCGACCTCAGATCAGACGTGGCG96020.1305355423865981No Hit
TTATAAAGCAATGAGACTGATT94950.12908091803382096No Hit
ATCCCGGACGAGCCCCCA92180.12531520826074372No Hit
TACCACCCTGAACGCGCCCGA90700.12330320448307068No Hit
CCCCCCACTGCTAAATTTGACTGT89130.12116884912432294No Hit
CTCCCACTGCTTCACTTGACTAGCCT88750.12065225355978526No Hit
TGTAAACATCCTACACTCTCAGCT88410.12019003647572525No Hit
ACCACAGGGTAGAACCACGGAC85150.1157581903167968No Hit
AGAGGTAGTAGGTTGCATAGTT84910.1154319194339309No Hit
ACTGGACTTGGAGTCAGAAGGCA82650.11235953528694369No Hit
CCCCCCACTGCTAAATTTGACTGTA82650.11235953528694369No Hit
TGAGGTAGTAGGTTGTATAGT82540.11220999446563014No Hit
AATGCACCCGGGCAAGGATTCT79630.10825396001088114No Hit
ACCGGGTGCTGTAGGCTT78780.10709841730073108No Hit
TTAGGTAGTAGGTTGTATAGTT78420.10660901097643222No Hit
CAAAGAATTCTCCTTTTGGGCTT77170.10490968346150567No Hit
CAAAGAATTCTCCTTTTGGGCT76490.10398524929338562No Hit
TATTGCACTTGTCCCGGCCTGTAAT75860.10312878822586265No Hit
TTCACAGTGGCTAAGTTCTGC74030.10064097274401018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)