FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003948008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6438613
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5228518.12055329307725No Hit
CAACGGAATCCCAAAAGCAGCTG4143216.434941811225492No Hit
AAGCTGCCAGTTGAAGAACTGT2443293.7947458559786087No Hit
TAGCAGCACGTAAATATTGGCG2043283.173478511598694No Hit
TTCAAGTAATCCAGGATAGGCT1788892.778377889772223No Hit
AACATTCAACGCTGTCGGTGAGT1695832.6338436554580933No Hit
CCCCCACTGCTAAATTTGACTG1521992.3638476174915315No Hit
GCCTGTCTGAGCGTCGCT1361722.114927547283864No Hit
TAGCTTATCAGACTGATGTTGAC1047981.627648687690967No Hit
AACATTCAACGCTGTCGGTGAG884631.3739449785225482No Hit
TAGCTTATCAGACTGATGTTGACT817741.270056144079478No Hit
TATTGCACTTGTCCCGGCCTGTAA780041.2115031606962554No Hit
TATTGCACTTGTCCCGGCCTGTAT736121.1432897116195677No Hit
TGAGGTAGTAGGTTGTATAGTT675481.0491079367559442No Hit
TATTGCACTTGTCCCGGCCTGT629620.9778814163857962No Hit
TGTAAACATCCTTGACTGGAAGCT626860.9735947788755126No Hit
GGAATACCGGGTGCTGTAGGCTTT556850.8648601802903824No Hit
TAGCTTATCAGACTGATGTTGA550230.8545784627838325No Hit
CGCCTGTCTGAGCGTCGCT484590.7526310402566515No Hit
AGCAGCATTGTACAGGGCTATGA430770.6690416088061202No Hit
TGAGGTAGTAGATTGTATAGTT404270.6278836761892662No Hit
TATTGCACTTGTCCCGGCCTGTA384580.5973025556901773No Hit
GGAATACCGGGTGCTGTAGGCTT380380.5907794116527891No Hit
TTCAAGTAATTCAGGATAGGTT354740.5509571704340671No Hit
CATAAAGTAGAAAGCACTACT347810.5401939827723766No Hit
ACTGGACTTGGAGTCAGAAGGC339270.5269302565630207No Hit
TCGTACGACTCTTAGCGG333690.5182637937704906No Hit
TTAATGCTAATCGTGATAGGGGTT316080.4909131826994416No Hit
TTCTCACTACTGCACTTGACTAG309620.48087996591812554No Hit
TGTAAACATCCCCGACTGGAAGCT299200.464696356187272No Hit
CAACGGAATCCCAAAAGCAGCTGT299100.46454104323400086No Hit
AACCGGGCGGAAACACCA284020.44111984988071185No Hit
CCCATAAAGTAGAAAGCACTAC230760.35840017096849897No Hit
CTACGCCTGTCTGAGCGTCGCT212800.33050596456100095No Hit
CACCCGGCTGTGTGCACATGTGC210400.3267784536824934No Hit
TACGCCTGTCTGAGCGTCGCT209760.32578445078155804No Hit
CTTCTCACTACTGCACTTGACTAG201240.31255178716285636No Hit
TGTAAACATCCTACACTCTCAGCT198190.3078147420880864No Hit
CCCCCACTGCTAAATTTGACTGGCTT195950.30433573193481267No Hit
CTCCCACTGCTTCACTTGACTAG184540.2866145239665748No Hit
AGCTCGGTCTGAGGCCCCTCAGT173830.2699805066712349No Hit
TGAGGTAGTAGTTTGTACAGTT173020.2687224717497386No Hit
CCCCCCACTGCTAAATTTGACTGG170960.265523024912353No Hit
CATTGCACTTGTCTCGGTCTGA170900.2654298371403903No Hit
AACATTCAACGCTGTCGGTGAGTT159350.24749119103757286No Hit
ATCCCGGACGAGCCCCCA150980.23449149684877782No Hit
ACGCCTGTCTGAGCGTCGCT150630.23394790151232883No Hit
CCCCCCACTGCTAAATTTGACTGGCT148610.23081057985625164No Hit
TAGCTTATCAGACTGATGTTGACA133940.20802616961137438No Hit
GCGTTCGCGCTTTCCCCTG129350.20089730505622871No Hit
TCCTGTCTGAGCGTCGCT128640.19979458308800357No Hit
CCCATAAAGTAGAAAGCACT122360.19004092962257554No Hit
ACCCCCCACTGCTAAATTTGACTG121650.1889382076543504No Hit
CAAAGAATTCTCCTTTTGGGCT112380.17454069688611507No Hit
CGCGACCTCAGATCAGACGTGGCG108630.16871646113844704No Hit
TGAGGTAGTAGTTTGTGCTGTT107930.167629270465549No Hit
AACGGAATCCCAAAAGCAGCTG107780.16739630103564232No Hit
TAGCAGCACGTAAATATTGGC107300.1666507988599408No Hit
TATTGCACTTGTCCCGGCCTGTT104870.1628766940954519No Hit
TTCAAGTAATCCAGGATAGGCTT100260.15571676694965203No Hit
GTCAGGATGGCCGAGCGGTCTAAGG99650.15476935793469804No Hit
CAAAGAATTCTCCTTTTGGGCTT99560.15462957627675403No Hit
TTCACAGTGGCTAAGTTCCG96310.14958190529544174No Hit
CAACACCAGTCGATGGGCTGT94700.14708136674777625No Hit
CAACGGAATCCCAAAAGCAGCT94440.1466775530692713No Hit
GGGCTACGCCTGTCTGAGCGTCGCT93560.1453107990804852No Hit
TGTAAACATCCCCGACTGGAAGC93490.14520208001319537No Hit
CCCCCCACTGCTAAATTTGACTGT92920.14431679617954984No Hit
TATTGCACTTGTCCCGGCCTGTAG89760.1394089068561816No Hit
GGAATACCGGGTGCTGTAGGCTTA89430.13889637411038683No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT88180.1369549621944975No Hit
CCCCCCACTGCTAAATTTGACTGTA87890.13650455463001115No Hit
TCGTTTCCCGGGCGGCGCACCA87860.1364579607440298No Hit
AGCAGCATTGTACAGGGCTATGT87480.13586777152159946No Hit
GGGAATACCGGGTGCTGTAGGCTTT87410.13575905245430966No Hit
TGTCAGTTTGTCAAATACCCCAA87160.13537077007113177No Hit
TGAGGTAGTAGGTTGTGTGGTT85430.13268385597954094No Hit
ACTGGACTTGGAGTCAGAAGGCA83270.12932909618888416No Hit
TAGCAGCACGTAAATATTGGCGT81770.12699940188981695No Hit
ACCACAGGGTAGAACCACGGAC81090.12594327380757317No Hit
CACTAGATTGTGAGCTCCTGGA80940.12571030437766642No Hit
TGTCAGTTTGTCAAATACCCCA80870.12560158531037663No Hit
CAAAGTGCTGTTCGTGCAGGTAG80010.12426589391224475No Hit
ATCCCACCGCTGCCACCA79660.12372229857579575No Hit
CTCCCACTGCTTCACTTGACTAGCCT79020.1227282956748604No Hit
TGAGAACTGAATTCCATGGGTT78180.12142366686738278No Hit
TTCAAGTAATCCAGGATAGGC76330.11855037723186655No Hit
TTATAAAGCAATGAGACTGATT75490.11724574842438891No Hit
GAATACCGGGTGCTGTAGGCTTT74520.11573921277765878No Hit
CCAAGTCCTTCTGATCGAGGCCC74200.11524221132719112No Hit
GTGCGGTAACGCGACCGATCCCGGA73950.11485392894401325No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC72550.11267954759821719No Hit
CACTAGATTGTGAGCTCCTGGAA72110.11199617060382414No Hit
TGTAAACATCCTACACTCAGCT71800.1115147004486836No Hit
AGCAGCATTGTACAGGGCTATG70970.11022560293653308No Hit
GGGAATACCGGGTGCTGTAGGCTT70850.11003922739260769No Hit
AGAGGTAGTAGGTTGCATAGTT69220.10750762625428799No Hit
ACTGGACTTGGAGTCAGAAGGCT66570.1033918329926026No Hit
TGATGTAGTAGGTTGTATAGTT65670.10199401641316228No Hit
CCTCGACACAAGGGTTTGT65440.10163679662063864No Hit
TATTGCACTTGTCCCGGCCTGTTT65160.10120192035147943No Hit
TTCACAGTGGCTAAGTTCTGC64710.10050301206175925No Hit
TCGTTTCCCGGCCAATGCACCA64540.10023898004119833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)