FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003948009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8305264
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT92089011.088028026562432No Hit
CAACGGAATCCCAAAAGCAGCTG86130510.370591470662461No Hit
CCCCCCACTGCTAAATTTGACTG6993638.42071967850751No Hit
CCCCCACTGCTAAATTTGACTG3433444.13405281277031No Hit
TTCAAGTAATCCAGGATAGGCT2568563.092689166774229No Hit
AAGCTGCCAGTTGAAGAACTGT2280312.7456201271868057No Hit
TAGCAGCACGTAAATATTGGCG2190992.638073877001381No Hit
AACATTCAACGCTGTCGGTGAGT1901402.2893914028500477No Hit
AACATTCAACGCTGTCGGTGAG1472451.7729117340520424No Hit
TGAGGTAGTAGGTTGTATAGTT1081411.3020778147449616No Hit
TCGTACGACTCTTAGCGG987071.188487205223097No Hit
TAGCTTATCAGACTGATGTTGACT893811.0761969757975183No Hit
TAGCTTATCAGACTGATGTTGAC836141.0067590867671394No Hit
TATTGCACTTGTCCCGGCCTGTAA810030.9753211938837827No Hit
TAGCTTATCAGACTGATGTTGA760990.9162743050672442No Hit
CATAAAGTAGAAAGCACTACT743940.8957451563249526No Hit
TATTGCACTTGTCCCGGCCTGT707110.8513997869303132No Hit
TATTGCACTTGTCCCGGCCTGTAT690710.8316532743570825No Hit
CAACGGAATCCCAAAAGCAGCTGT474610.5714568495354272No Hit
TGAGGTAGTAGATTGTATAGTT465110.5600183209106899No Hit
TGTAAACATCCTTGACTGGAAGCT438820.5283636980112855No Hit
CACCCGGCTGTGTGCACATGTGC408020.4912787841542424No Hit
CGCCTGTCTGAGCGTCGCT406950.4899904446144036No Hit
TTCAAGTAATTCAGGATAGGTT406330.48924393011468387No Hit
CCCATAAAGTAGAAAGCACTAC406120.488991078429295No Hit
TATTGCACTTGTCCCGGCCTGTA393240.47348284172544064No Hit
TTAATGCTAATCGTGATAGGGGTT346450.4171450781094978No Hit
AGCAGCATTGTACAGGGCTATGA328980.3961102259964283No Hit
CATTGCACTTGTCTCGGTCTGA322540.3883561076445011No Hit
CAACACCAGTCGATGGGCTGT308660.3716438152959376No Hit
CCCATAAAGTAGAAAGCACT298110.3589410282442557No Hit
AACCGGGCGGAAACACCA291760.35129527490035234No Hit
TGAGAACTGAATTCCATGGGTT276610.3330538318830082No Hit
ACTGGACTTGGAGTCAGAAGGC272370.32794863594944124No Hit
TTCTCACTACTGCACTTGACTAG266010.3202908420490908No Hit
TGATGTAGTAGGTTGTATAGTT249640.3005804511452014No Hit
TGTAAACATCCCCGACTGGAAGCT227090.27342899635700924No Hit
CAACGGAATCCCAAAAGCAGCT205220.24709629940721933No Hit
CCCCCACTGCTAAATTTGACTGGCTT200870.24185865735273435No Hit
AACGGAATCCCAAAAGCAGCTG199600.2403295066839537No Hit
CTCCCACTGCTTCACTTGACTAG199430.24012481722435314No Hit
CTACGCCTGTCTGAGCGTCGCT197000.23719896200770982No Hit
TGAGGTAGTAGTTTGTACAGTT175460.2112636034206739No Hit
TAGCAGCACGTAAATATTGGC160530.19328705264516577No Hit
CCCCCCACTGCTAAATTTGACTGG159160.19163749641191416No Hit
TGTAAACATCCTACACTCTCAGCT155280.18696576051044253No Hit
AGCTCGGTCTGAGGCCCCTCAGT154560.1860988404462519No Hit
CTTCTCACTACTGCACTTGACTAG152330.18341379635855043No Hit
ATCCCGGACGAGCCCCCA151840.18282380909264295No Hit
TTCACAGTGGCTAAGTTCCGC142900.1720595516289428No Hit
ACCCCCCACTGCTAAATTTGACTG139780.16830289801745013No Hit
AACATTCAACGCTGTCGGTGAGTT132940.1600671574076393No Hit
ACGCCTGTCTGAGCGTCGCT116760.14058553707624466No Hit
TACGCCTGTCTGAGCGTCGCT116480.14024840149572607No Hit
TATTGCACTTGTCCCGGCCTGTT111970.13481811053808765No Hit
TGAGGTAGTAGTTTGTGCTGTT110630.13320467597417734No Hit
TGAGGTAGTAGGTTGTGTGGTT110430.1329638648452355No Hit
CAAAGAATTCTCCTTTTGGGCT108710.1308928891363357No Hit
CACTAGATTGTGAGCTCCTGGA108700.13088084857988863No Hit
TTCAAGTAATCCAGGATAGGC108030.13007413129793344No Hit
TGATGTAGTAGATTGTATAGTT107230.12911088678216612No Hit
TTCACAGTGGCTAAGTTCTGC105600.12714827608129015No Hit
CACCCGTAGAACCGACCTTGCG100600.1211279978577442No Hit
CCCATAAAGTAGAAAGCACTA99470.11976741497922283No Hit
TCGTTTCCCGGCCAATGCACCA99250.11950252273738679No Hit
TTCAAGTAATCCAGGATAGGCTT98980.11917742771331531No Hit
CCCCCCACTGCTAAATTTGACTGGCT98810.11897273825371477No Hit
TTCACAGTGGCTAAGTTCCG98520.11862356211674908No Hit
TAGCTTATCAGACTGATGTTGACA98490.11858744044740781No Hit
GGAATACCGGGTGCTGTAGGCTTT95780.11532444965024592No Hit
GCCGGTCTGAGCGTCGCT90440.10889479250749887No Hit
ACCGGGTGCTGTAGGCTT87550.1054150716942893No Hit
ACCACAGGGTAGAACCACGGAC86980.10472875997680509No Hit
TGAGAACTGAATTCCATAGGCTGT86890.10462039496878124No Hit
TACCACCCTGAACGCGCCCGA86540.10419897549313302No Hit
TATTGCACTTGTCCCGGCCTGTAG86100.10366919100946098No Hit
TGAGGTAGTAGGTTGTATAGT84940.10227248646159832No Hit
AGAGGTAGTAGGTTGCATAGTT84830.10214004034068032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)