FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003948010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11466539
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG10365909.040129720048919No Hit
CAACGGAATCCCAAAAGCAGCTG8509227.420914017734558No Hit
CCCCCACTGCTAAATTTGACTG3962223.455462890764162No Hit
TTCAAGTAATCCAGGATAGGCT3495753.048653128899662No Hit
TAGCAGCACGTAAATATTGGCG2848352.4840538195527No Hit
AACATTCAACGCTGTCGGTGAGT2726832.378075895438022No Hit
AAGCTGCCAGTTGAAGAACTGT2537782.2132048737635657No Hit
GGAATACCGGGTGCTGTAGGCTTT2265271.975548157992573No Hit
AACATTCAACGCTGTCGGTGAG2205391.9233266463402776No Hit
TAGCTTATCAGACTGATGTTGAC1559181.359765139245591No Hit
GGAATACCGGGTGCTGTAGGCTT1452101.2663803785954943No Hit
TCGTACGACTCTTAGCGG1230591.0732009022077194No Hit
TGAGGTAGTAGGTTGTATAGTT1229141.0719363532448631No Hit
TAGCTTATCAGACTGATGTTGACT1118150.9751416709087197No Hit
TAGCTTATCAGACTGATGTTGA1014860.8850621796167092No Hit
TATTGCACTTGTCCCGGCCTGT998280.8706027163034983No Hit
TTAATGCTAATCGTGATAGGGGTT965090.8416576265950868No Hit
TATTGCACTTGTCCCGGCCTGTAA869110.7579532062813373No Hit
TTCAAGTAATTCAGGATAGGTT868210.7571683138216335No Hit
TGAATACCGGGTGCTGTAGGCTTT837810.7306563907383039No Hit
CATAAAGTAGAAAGCACTACT831570.7252144696843573No Hit
TATTGCACTTGTCCCGGCCTGTAT787910.687138464361391No Hit
TGTAAACATCCTTGACTGGAAGCT782560.6824727147398182No Hit
TATTGCACTTGTCCCGGCCTGTA649100.5660818839930689No Hit
TGAGGTAGTAGATTGTATAGTT593600.5176801823113322No Hit
CAACGGAATCCCAAAAGCAGCT558540.48710426049220257No Hit
TGAATACCGGGTGCTGTAGGCTT534790.46639182058335127No Hit
CAACGGAATCCCAAAAGCAGCTGT470060.4099406106759852No Hit
AGCAGCATTGTACAGGGCTATGA461170.4021876173795772No Hit
TTCTCACTACTGCACTTGACTAG424420.37013784194167043No Hit
TGTAAACATCCCCGACTGGAAGCT404740.35297486015614654No Hit
TGAGAACTGAATTCCATGGGTT403070.35151844859202935No Hit
CATTGCACTTGTCTCGGTCTGA392480.34228288064951423No Hit
CACCCGGCTGTGTGCACATGTGC386480.3370502642514886No Hit
GGAATACCGGGTGCTGTAGGCTTA384170.33503570693824875No Hit
CGCCTGTCTGAGCGTCGCT365530.31877971199504923No Hit
CTACGCCTGTCTGAGCGTCGCT351940.3069278358535213No Hit
CCCCCACTGCTAAATTTGACTGGCTT340150.296645744631401No Hit
ATACCGGGTGCTGTAGGCTTT338030.29479688683743194No Hit
CCCATAAAGTAGAAAGCACTAC335130.29226778891171956No Hit
GCCTGTCTGAGCGTCGCT331220.28885786722567286No Hit
CTCCCACTGCTTCACTTGACTAG329120.2870264514863639No Hit
ATCCCGGACGAGCCCCCA317730.27709320135744536No Hit
ACCGGGTGCTGTAGGCTT317150.2765873817723029No Hit
ACTGGACTTGGAGTCAGAAGGC311480.27164255927616865No Hit
ATACCGGGTGCTGTAGGCTTA305510.26643610596013323No Hit
TGATGTAGTAGGTTGTATAGTT299640.2613168629173982No Hit
TCGTTTCCCGGCCAATGCACCA295840.25800287253198195No Hit
GGGAATACCGGGTGCTGTAGGCTTT295010.2572790272635884No Hit
CAACACCAGTCGATGGGCTGT284570.24817427473102388No Hit
ACCGGGTGCTGTAGGCTTT283980.24765973411855138No Hit
ATACCGGGTGCTGTAGGCTT279990.24418004421386436No Hit
TAGCAGCACGTAAATATTGGC259610.2264065905152374No Hit
AACCGGGCGGAAACACCA250050.21806928838771664No Hit
CCCATAAAGTAGAAAGCACT244200.21296748739964166No Hit
CCCCCCACTGCTAAATTTGACTGG242230.2112494450156233No Hit
TGAGGTAGTAGTTTGTACAGTT234500.2045080908895003No Hit
CTTCTCACTACTGCACTTGACTAG233140.2033220311726145No Hit
GGGAATACCGGGTGCTGTAGGCTT229860.20046153420836052No Hit
AACATTCAACGCTGTCGGTGAGTT223060.19453123562393151No Hit
TAGCTTATCAGACTGATGTTGACA221220.19292656659520366No Hit
ACCCCCCACTGCTAAATTTGACTG217550.18972594956507802No Hit
CAAAGAATTCTCCTTTTGGGCT212760.1855485774739876No Hit
GAATACCGGGTGCTGTAGGCTTT211520.18446717008506228No Hit
GGAATACCGGGTGCTGTAGGCT210750.18379565098064898No Hit
CTCCTGGCTGGCTCGCCA207300.1807868965517843No Hit
TTCACAGTGGCTAAGTTCCG201140.175414743716478No Hit
AACGGAATCCCAAAAGCAGCTG200710.17503973954128615No Hit
TGTAAACATCCTACACTCTCAGCT199690.1741501947536218No Hit
ACCGGGTGCTGTAGGCTTA198360.17299029811872613No Hit
AGCTCGGTCTGAGGCCCCTCAGT198340.17297285606406607No Hit
CCCCCCACTGCTAAATTTGACTGGCT196810.17163853888256952No Hit
CTCCCACTGCTTCACTTGACTAGCCT176380.15382148004729237No Hit
AGGTCCCTGTTCGGGCGCCA174390.15208599560861388No Hit
TATTGCACTTGTCCCGGCCTGTT172880.15076912048177746No Hit
CACCCGTAGAACCGACCTTGCG168810.1472196623584501No Hit
TGAGGTAGTAGGTTGTGTGGTT163900.14293763793939915No Hit
TACGCCTGTCTGAGCGTCGCT161500.1408445913801889No Hit
GAATACCGGGTGCTGTAGGCTT158020.13780967386933407No Hit
TTCAAGTAATCCAGGATAGGC157900.13770502154137357No Hit
TGAATACCGGGTGCTGTAGGCTTA155810.13588232682939463No Hit
CACTAGATTGTGAGCTCCTGGA154920.13510615539702084No Hit
TGTCAGTTTGTCAAATACCCCA154590.13481836149512944No Hit
TGAGGTAGTAGTTTGTGCTGTT153990.1342950998553269No Hit
TGTCAGTTTGTCAAATACCCCAA153640.1339898638987754No Hit
CTCGACACAAGGGTTTGT148730.12970783947972445No Hit
TGATGTAGTAGATTGTATAGTT146950.12815549661497685No Hit
AATACCGGGTGCTGTAGGCTT144390.12592291361848593No Hit
AATACCGGGTGCTGTAGGCTTT141110.12306241665423193No Hit
TACCGGGTGCTGTAGGCTTT140840.12282694891632079No Hit
TTAATGCTAATCGTGATAGGGGT134440.11724549142509348No Hit
TTCACCACCTTCTCCACCCAGC132660.11569314856034589No Hit
ATCCCACCGCTGCCACCA130680.11396638514899744No Hit
TTCAAGTAATCCAGGATAGGCTT130570.11387045384836697No Hit
TGAGAACTGAATTCCATAGGCTGT129950.11332975015390433No Hit
CAACGGAATCCCAAAAGCAGCTGA128340.11192566475376746No Hit
CAAAGTGCTGTTCGTGCAGGTAG126040.10991982846785765No Hit
TTCACAGTGGCTAAGTTCTG125850.10975412894858685No Hit
CACTAGATTGTGAGCTCCTGGAA120140.10477442234313249No Hit
ACGCCTGTCTGAGCGTCGCT119430.10415522940269946No Hit
AATGCACCCGGGCAAGGATTCT119380.10411162426604922No Hit
TACCGGGTGCTGTAGGCTT119090.103858714473478No Hit
AGCTACATTGTCTGCTGGGTTT119030.10380638830949775No Hit
ATCCCATCCTCGTCGCCA117770.10270753886591237No Hit
TCCTGTCTGAGCGTCGCT116380.10149531606703645No Hit
TTCACAGTGGCTAAGTTCTGC116120.10126856935645534No Hit
TTATAAAGCAATGAGACTGATT115040.10032669840481073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)