FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003948011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7152498
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT6560979.172977049416861No Hit
CAACGGAATCCCAAAAGCAGCTG6470309.046210149237371No Hit
CCCCCCACTGCTAAATTTGACTG6192458.65774446913512No Hit
CCCCCACTGCTAAATTTGACTG3098654.332262658444644No Hit
TTCAAGTAATCCAGGATAGGCT2276913.1833773319475243No Hit
TAGCAGCACGTAAATATTGGCG2025202.831458324070835No Hit
AAGCTGCCAGTTGAAGAACTGT1834512.5648521677321687No Hit
AACATTCAACGCTGTCGGTGAGT1585712.217001668507981No Hit
AACATTCAACGCTGTCGGTGAG1314791.8382249110730264No Hit
TGAGGTAGTAGGTTGTATAGTT896481.2533802875582767No Hit
TAGCTTATCAGACTGATGTTGACT873261.2209161051146047No Hit
TATTGCACTTGTCCCGGCCTGTAA829061.1591195132106293No Hit
TAGCTTATCAGACTGATGTTGAC810111.132625273016504No Hit
TAGCTTATCAGACTGATGTTGA746141.0431879883084203No Hit
CATAAAGTAGAAAGCACTACT702610.9823281320735776No Hit
TATTGCACTTGTCCCGGCCTGTAT684990.9576933820883278No Hit
TATTGCACTTGTCCCGGCCTGT575790.8050194491490945No Hit
TCCTGTCTGAGCGTCGCT493500.689968735398458No Hit
TGTAAACATCCTTGACTGGAAGCT445100.6223000691506659No Hit
TGAGGTAGTAGATTGTATAGTT427550.5977631870711464No Hit
TCGTACGACTCTTAGCGG404730.5658582498030758No Hit
TATTGCACTTGTCCCGGCCTGTA396950.5549809311376249No Hit
TTCAAGTAATTCAGGATAGGTT354630.49581279155897706No Hit
CCCATAAAGTAGAAAGCACTAC351400.49129688676599426No Hit
CAACGGAATCCCAAAAGCAGCTGT345030.4823909073445389No Hit
TTCTCACTACTGCACTTGACTAG338570.4733590977585733No Hit
CACCCGGCTGTGTGCACATGTGC305330.42688582366608147No Hit
CCCCCACTGCTAAATTTGACTGGCTT294430.41164639263093816No Hit
AGCAGCATTGTACAGGGCTATGA282480.3949389430098408No Hit
ACTGGACTTGGAGTCAGAAGGC237670.33228950221307296No Hit
CATTGCACTTGTCTCGGTCTGA237010.33136674767333035No Hit
CCCATAAAGTAGAAAGCACT232300.32478163573062163No Hit
CTCCCACTGCTTCACTTGACTAG231950.32429229620197025No Hit
CGCCTGTCTGAGCGTCGCT228600.319608617856307No Hit
CAACACCAGTCGATGGGCTGT220980.30895499726109676No Hit
TGTAAACATCCCCGACTGGAAGCT214530.29993716880452115No Hit
CTTCTCACTACTGCACTTGACTAG194290.27163936291908086No Hit
AACCGGGCGGAAACACCA186650.26095778006509057No Hit
CAACGGAATCCCAAAAGCAGCT164700.23026920105395343No Hit
TGATGTAGTAGGTTGTATAGTT160130.22387982492270533No Hit
TGAGGTAGTAGTTTGTACAGTT159810.22343242878222405No Hit
AACGGAATCCCAAAAGCAGCTG152150.21272288366945366No Hit
TAGCAGCACGTAAATATTGGC144080.20144011225169167No Hit
CCCCCCACTGCTAAATTTGACTGG143080.2000419993126877No Hit
TTCACAGTGGCTAAGTTCCG134970.18870330337736552No Hit
ACCCCCCACTGCTAAATTTGACTG133730.1869696433330006No Hit
CCCCCCACTGCTAAATTTGACTGGCT124150.17357572137734256No Hit
AGCTCGGTCTGAGGCCCCTCAGT123450.1725970423200398No Hit
TTCACAGTGGCTAAGTTCCGC121170.16940934481911074No Hit
CAAAGAATTCTCCTTTTGGGCT120890.16901787319618963No Hit
GTCAGGATGGCCGAGCGGTCTAAGG116630.16306191207603274No Hit
TGTAAACATCCTACACTCTCAGCT115920.1620692518893399No Hit
AACATTCAACGCTGTCGGTGAGTT114610.1602377239392447No Hit
TAGCTTATCAGACTGATGTTGACA110800.1549109136416396No Hit
TTCAAGTAATCCAGGATAGGC110500.15449147975993843No Hit
CTCCCACTGCTTCACTTGACTAGCCT109210.15268791406862328No Hit
TATTGCACTTGTCCCGGCCTGTT106830.14936040527379385No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC100850.14099968989855013No Hit
TTCACAGTGGCTAAGTTCTGC97040.135672879600945No Hit
CTACGCCTGTCTGAGCGTCGCT94670.1323593519355056No Hit
CCCATAAAGTAGAAAGCACTA93900.13128280497247255No Hit
TTCACAGTGGCTAAGTTCTG93200.13030412591516977No Hit
TGAGGTAGTAGTTTGTGCTGTT91980.12859842812958494No Hit
TGAGGTAGTAGGTTGTGTGGTT86010.12025169388373125No Hit
CACTAGATTGTGAGCTCCTGGA85140.11903533562679779No Hit
TATTGCACTTGTCCCGGCCTGTAG82780.11573578909074844No Hit
AGAATTACTCTGAGACCT79520.1111779409095955No Hit
TTCAAGTAATCCAGGATAGGCTT79320.11089831832179471No Hit
TGATGTAGTAGATTGTATAGTT77350.10814403583195688No Hit
TCGTTTCCCGGCCAATGCACCA76000.10625658336430155No Hit
ATCCCGGACGAGCCCCCA74710.10445301767298641No Hit
TGAGGTAGTAGGTTGTATAGT74490.10414543282640555No Hit
CACTAGATTGTGAGCTCCTGGAA74280.10385182910921471No Hit
CAAAGAATTCTCCTTTTGGGCTT73930.10336248958056332No Hit
TTATAAAGCAATGAGACTGATT73320.10250964068777091No Hit
ATCACATTGCCAGGGATTTCC71950.10059422596133547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)