FastQCFastQC Report
Tue 28 Jun 2022
EGAF00003948231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355851576
Sequences flagged as poor quality0
Sequence length98
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6543460.1838817203945726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5923130.16644945250994195Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG5007120.14070810241402443No Hit
GCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCT4662850.1310335632741444No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC4494800.12631108875572325No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC4151760.11667111458851598No Hit
GCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACC3987560.11205683124472098No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC3863810.10857925777459532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)