FastQCFastQC Report
Sat 4 May 2024
EGAF00003948361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450449747
Total Bases68 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATCTCATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT25983290.5768299388122422No Hit
CTTGATCTCATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA23493090.5215474124797322No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC14864340.32998886333040833Illumina Single End PCR Primer 1 (96% over 33bp)
CTTGATCTCATGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC6018900.13361978866867918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)