FastQCFastQC Report
Sun 12 May 2024
EGAF00003948363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410067987
Total Bases61.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC35936060.8763439512287508Illumina Single End PCR Primer 1 (96% over 33bp)
GGCTTAAGAGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT24102490.5877681448954463No Hit
GGCTTAAGAGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA20010150.48797152263436744No Hit
GGCTTAAGAGAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC7287720.1777197984489338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)