FastQCFastQC Report
Sun 15 May 2022
EGAF00003948456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498530164
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG59686631.197252128559266TruSeq Adapter, Index 7 (97% over 35bp)
CATCAGCGAACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT29759010.5969349930849921No Hit
CATCAGCGAACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA19340970.38795987478101723No Hit
CATCAGCGAACGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC7185980.14414333412330893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)