FastQCFastQC Report
Thu 16 Jun 2022
EGAF00003948457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498530164
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC62127301.246209447017533Illumina Single End PCR Primer 1 (96% over 31bp)
CATCAGCGAACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT24278800.4870076427311226No Hit
CATCAGCGAACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA20571860.4126502564045452No Hit
CATCAGCGAACGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC6331860.1270105694146122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)