FastQCFastQC Report
Thu 2 May 2024
EGAF00003948459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464883680
Total Bases70.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC22546920.48500132334178736Illumina Single End PCR Primer 1 (96% over 33bp)
TCACCTGTCGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT10176580.21890594223484033No Hit
TCACCTGTCGGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA8534380.18358097664344766No Hit
CACCTGTCGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGTA4654190.10011515138582625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)