FastQCFastQC Report
Tue 7 May 2024
EGAF00003948474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477925197
Total Bases72.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGAGATCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT66425731.3898771275706563No Hit
CCCGAGAGATCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA47099620.9855019215486143No Hit
CCCGAGAGATCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC14294430.29909345834302187No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG9857360.2062531974015172TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)