FastQCFastQC Report
Mon 6 May 2024
EGAF00003948475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477925197
Total Bases72.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGAGATCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT55263521.1563215404188032No Hit
CCCGAGAGATCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA49349281.032573304562555No Hit
CCCGAGAGATCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC12735030.26646492128767174No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC10535870.22045018898637392Illumina Single End Sequencing Primer (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)