FastQCFastQC Report
Mon 13 May 2024
EGAF00003948746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318093371
Total Bases31.1 Gbp
Sequences flagged as poor quality0
Sequence length98
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5778300.18165420995208353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4477800.14076998794168522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGG3968600.12476210955053194No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA3658910.11502628893200041No Hit
CCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAA3458980.10874102748906389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)