FastQCFastQC Report
Mon 13 May 2024
EGAF00003948754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003948754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330626758
Total Bases32.4 Gbp
Sequences flagged as poor quality0
Sequence length98
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5726840.17321163098359993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5267290.15931227199705356Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA3557280.10759201770353989No Hit
GTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTT3472960.10504170990298371No Hit
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAA3437370.10396526950187135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)