FastQCFastQC Report
Wed 14 Sep 2022
EGAF00003952320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23591763
Sequences flagged as poor quality0
Sequence length35-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC6733982.8543776062857193TruSeq Adapter, Index 4 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3312101.4039222079333367No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGC1871820.7934209918945014TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGC1295550.5491535329513101TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGC1156730.4903109614995708TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGG683070.2895374966254112TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC668550.2833828061090644TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGATGC607160.2573610119769345TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTTTGC464620.19694161898794932TruSeq Adapter, Index 4 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG413820.1754086797158822TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)