FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17305632
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT507200.29308377758177223No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG477630.2759968546655794No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC395110.22831295615207814Illumina Single End PCR Primer 1 (96% over 33bp)
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC391350.22614025306905866No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGA318480.18403257390426422No Hit
GCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTT314930.18198121859981767No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC223170.12895801782910904No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT208700.12059657803887196No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA202810.1171930617731846No Hit
GTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAAC200980.11613560255990651No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG192940.11148971618025853No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG187680.10845024325028985No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG183400.10597705995366133No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT182410.10540499185467482No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC178040.10287980236722935No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG176380.10192057706993884No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC175220.10125027505496476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG143700.050.08972
CGCCGTA60550.040.9956456-57
CGTATCA66450.040.21087660-61
GTGGCGC186650.038.9707341
GGCGATC28450.036.4291761
GGCGCGT201100.035.9161723
TGGTCGC78550.033.16729452-53
TCGCCGT75100.032.57863256-57
GTGGTCG83050.032.48540552-53
TCGGTGG95150.032.19923848-49
GATCTCG97300.031.60960644-45
TCTCGGT92150.031.5204246-47
GTAGATC98550.031.2061942-43
GCGATCT47900.031.06591
ATCGGGA29900.030.8464851
GTATCAT87700.030.44056760-61
GGTCGCC90200.030.20594654-55
TAGATCT101050.030.08162342-43
CGATCTG49150.029.869472
ATCATTA106500.028.67731362-63
Produced by FastQC (version 0.11.5)