FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16509092
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC350620.21237994191321968Illumina Single End PCR Primer 1 (96% over 33bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT242520.1469008713501627No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG226860.13741518915758663No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC208460.12626981544472587No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA206730.12522190802498406No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC188130.11395538894567914No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG169370.10259195357321892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA58500.039.09695460-61
CGCCGTA52750.037.2803856-57
TGGCGCG85000.036.768892
TCGCCGT60700.035.29314456-57
GGTCGCC73550.032.3533654-55
GTGGTCG74100.031.92092552-53
TGGTCGC66500.031.46216652-53
GCCGTAT77200.029.68810858-59
TCTCGGT81350.029.4572146-47
GGCGCGT106900.029.14733
TCGGTGG85750.029.1366548-49
GTAGATC94650.028.72947942-43
CTCGGTG95100.027.72046348-49
GTCTAAC40950.027.5103191
CGGTGGT93450.026.8869450-51
CCGTATC77900.026.15907358-59
TAGGGTG96400.025.6945136-37
GATCTCG98950.025.58582744-45
GGGTGTA98900.025.09298938-39
GATAGGG107700.024.43237134-35
Produced by FastQC (version 0.11.5)