FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19756017
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG1606970.8134078847978315TruSeq Adapter, Index 7 (97% over 34bp)
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT449900.22772808911836834No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT383930.19433573072952914No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG312940.15840237432474372No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC311310.15757730923191654No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC276990.14020538654122436No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG271220.13728475734759693No Hit
GCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCG264620.1339440029840023No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT264280.13377190351678683No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG264180.13372128602642933No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC243880.12344593548385792No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC237850.12039370081530097No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT235040.11897134933625537TruSeq Adapter, Index 7 (97% over 35bp)
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG234720.11880937336711139No Hit
TGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGC228390.11560528622748198No Hit
GTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG226540.11466886265586833No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT221150.11194057992559939No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT219480.11109526783662921No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC218630.1106650191685905No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC210230.10641314997856097No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG207200.10487944002072888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG42400.048.1798062
CGTACGG44450.045.850913
TCGTATG228450.043.9049544-45
GGCGTAC47850.043.287951
TATCTCG215550.042.2687240-41
CGTATCT218850.041.57835438-39
ATGCCGT241000.041.1736248-49
AATTCGT243750.040.84917434-35
TCTCGTA224400.040.72665442-43
GCCGTCT243550.040.5366150-51
GTATGCC250800.039.96299446-47
TATGCCG224350.039.28601546-47
CGAATTC256700.038.9439432-33
CACGAAT271850.036.9037930-31
TCGTATC216900.036.7180436-37
GTCACGA273900.036.71748728-29
CGTATGC245550.035.9047244-45
CGTCTTC278450.035.56703652-53
ACGAATT249100.035.38291530-31
AGCACAC516650.034.4875649
Produced by FastQC (version 0.11.5)