FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19756017
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC1187670.6011687477288564Illumina Single End PCR Primer 1 (96% over 33bp)
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC349750.17703467252533747No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC259460.1313321404815556No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT247240.1251466831598697Illumina Single End PCR Primer 1 (97% over 34bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA200300.10138683318606173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA48550.050.5361671
CGTATCA167400.044.58023560-61
GTCTAAC48100.041.4200631
TAGTGTA232750.040.31606338-39
GTAGATC229350.040.155142-43
TCGCCGT171750.039.74949656-57
TATAGTG242650.038.2984836-37
GCTATAG219900.038.2412334-35
GTGGTCG201400.038.11238552-53
TCATTAA235950.037.6542764-65
CGCCGTA158300.037.3645856-57
GTGTAGA253150.037.11430740-41
GTAGGCT231850.036.55463830-31
TATCATT204700.036.4646462-63
TGGCGCG77100.035.9266852
AGGCTAT243650.034.804132-33
GATCTCG229600.034.42595344-45
TAGATCT232050.034.34567642-43
CTCGGTG260800.033.6948748-49
ATCATTA221850.033.6457662-63
Produced by FastQC (version 0.11.5)