FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17523623
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT807620.460875014259323No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC491880.2806953790320643No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG486350.27753963892055883TruSeq Adapter, Index 7 (97% over 37bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG428230.24437298154611065No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG407150.23234350567802103No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT391000.22312737497262983No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC378100.21576588357327706No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT299300.17079801362994398No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA280700.16018377021692376No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA276650.1578726043124758No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG264130.15072796304736755No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG255730.1459344337640681No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT249750.14252189744095728No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT244600.13958300746369628No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC237030.13526312452624667No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC227340.12973344610301193No Hit
GTCAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG212870.12147602125428059No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT201750.1151303015363889No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA200480.11440556556141386No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT198960.11353816502443588No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG198510.11328136881283055No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA195710.11168352571839738No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC194190.1108161251814194No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCC193050.1101655747786859No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA192450.10982317982987878No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC184280.10516090194362204No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA179960.10269565831221089No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT176350.10063558203688816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG20200.047.7379652
CGTACGG21200.045.7102473
GATCGTT55550.040.9554025
TATGCCG75600.039.99397746-47
TCGTATG79800.039.2290644-45
GGCGTAC27100.036.810291
ATCGTTT66600.034.3054246
ATGCCGT91850.033.69376448-49
GCCGTCT92050.033.46421450-51
GTATGCC95100.033.19192546-47
TCTCGTA80350.032.66269342-43
GTACGGA38050.032.5840531
GAGTCTT246700.032.208684
CGTATGC94300.032.1643444-45
TGGAGTC258700.031.142152
AGTCTTG258400.030.8431825
GGAGTCT264500.030.6208973
ACCGCGT15150.030.4124266
TACGGAA41150.030.013922
TGCCGTC100350.029.96405648-49
Produced by FastQC (version 0.11.5)