FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16691560
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT352170.21098687001095162No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG301930.18088782594317127No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC287080.17199111407202203Illumina Single End PCR Primer 1 (96% over 32bp)
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGC267030.15997905528302928No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA208190.12472770669727695No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC199290.11939567062635248No Hit
GCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGG175450.10511300321839302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG109500.050.002652
GTCTAAC32050.040.314681
CGTATCA49950.035.95079460-61
CGCCGTA46500.034.27977856-57
GTGGCGC173550.032.051961
GGCGCGT174450.031.2759133
GGCGATC30750.028.7336041
GTAGATC78350.027.76493842-43
TCTAACA47700.027.0787332
GTATCAT63500.026.85795660-61
GTGGTCG66800.026.77609452-53
GCGATCT48300.026.751251
TCGCCGT61600.026.30099156-57
TGGTCGC62100.026.16334352-53
TAGATCT80550.026.06315442-43
ATCATTA74350.026.00576262-63
TCGGTGG80200.025.5560448-49
GATCTCG80650.025.44106544-45
TCATTAA80500.025.22840964-65
CGATCTG52000.024.2916032
Produced by FastQC (version 0.11.5)