FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13708923
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT286490.20898067630841605No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG281550.2053771838969407No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC254020.18529537294796972Illumina Single End PCR Primer 1 (96% over 32bp)
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA222800.1625218844689696No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC197630.14416158001616902No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC171470.12507911817726308No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGA156670.11428322998094016No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT155890.11371425749491773No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG150950.11011076508344238No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT149400.1089801146304491No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG148400.10825066272529213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG98750.040.7810972
CGCCGTA31700.037.46925456-57
GTGGCGC111100.036.813851
CGTATCA36400.036.2822460-61
GGCGATC25300.034.3599661
GGCGCGT145250.030.4477861
GTAGATC56950.030.10799442-43
TCTCGGT53600.029.46606346-47
TCGCCGT42050.028.47271356-57
GATCTCG59950.028.24377844-45
GTACGTC56150.028.21124830-31
TAGATCT60150.028.19037842-43
TCGGTGG60000.027.35223648-49
GTGGTCG50650.027.24741752-53
TGGTCGC48650.027.0492752-53
GCGATCT36100.026.5806981
ATCGGGA19850.026.084841
GGGCGAT16800.025.730861
GTGTACG63800.025.46182628-29
TGTACGT59700.025.420328-29
Produced by FastQC (version 0.11.5)