FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003952683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003952683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22200882
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT492770.2219596500715602No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG442590.19935694446734142No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC372050.16758343204562773Illumina Single End PCR Primer 1 (96% over 31bp)
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC272530.12275638418329507No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA258880.11660797980909048No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC252570.11376575038775487No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT244660.11020282887860042No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGA239530.10789210987203121No Hit
GGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT239330.10780202336105385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG151600.045.5696372
GTGGCGC174100.039.844331
CGCCGTA52900.038.2574456-57
CGTATCA62050.035.55980760-61
GGCGATC31050.034.912681
GGCGCGT200300.034.422913
TCGCCGT66150.030.84574756-57
GGTCGCC78450.029.70349554-55
TGGTCGC73100.029.59784552-53
GTGGTCG79850.029.148152-53
TCTCGGT87450.028.73397646-47
TCGGTGG93000.028.27097948-49
GTAGATC101950.027.57974642-43
CTCGGTG101650.026.28580948-49
GATCTCG102300.025.72387744-45
GCGATCT54100.025.3986471
TAGTGTA114900.025.05232638-39
CGATCTG55300.024.6585312
CGCGTGC298100.023.256955
AGATTAG122650.023.15782434-35
Produced by FastQC (version 0.11.5)